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EC number: 287-477-0 | CAS number: 85535-85-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Vapour pressure
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- Endpoint summary
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
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- Long-term toxicity to aquatic invertebrates
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- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- polychlorinated tetradecane
- Molecular formula:
- Variable
- IUPAC Name:
- polychlorinated tetradecane
Constituent 1
- Specific details on test material used for the study:
- Chlorinated tetradecanes with levels of chlorination of 41.3, 45.5, 50.0, 55.0 and 60.2%.
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Secondary activated sludge (02-07-2010) was obtained from the wastewater treatment plant Nieuwgraaf in Duiven, The Netherlands. This plant is an activated sludge plant treating predominantly domestic wastewater. The activated sludge was preconditioned to reduce the endogenous respiration rates. To this end, 400 mg dry weight (DW)/L of activated sludge was aerated for one week. The sludge was diluted in the BOD bottles (van Ginkel and Stroo, 1992).
- Duration of test (contact time):
- 56 d
Initial test substance concentration
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- The Closed Bottle tests were performed according to Test Guidelines (OECD 1992). The nutrient medium of the Closed Bottle test contained per liter of deionized water: 8.5 mg KH2PO4, 21.75 mg K2HPO4, 33.4 mg Na2HPO4·2H2O, 22.5 mg MgSO4·7H2O, 27.5 mg CaCl2, 0.25 mg FeCl3·6H2O. Ammonium chloride was omitted from the medium to prevent nitrification. The test substances were added to the bottles using stock emulsions of 1.0 g/L prepared with the help of polyalkoxylate alkylphenol. The tests were performed in 0.3 L BOD (biological oxygen demand) bottles with glass stoppers. Use was made of 3 bottles containing only inoculum, and series of 3 bottles containing the respective test substances, polyalkoxylate alkylphenol and inoculum. Controls with polyalkoxylate alkylphenol but without test substance were included. The concentration of the test substances and polyalkoxylate alkylphenol in the bottles was 2.0 mg/L. The activated sludge was diluted to 2 mg DW/L in the mineral salts medium and the river water spiked with nutrient medium salts was used undiluted. Each of the prepared solutions was dispensed into the respective group of BOD bottles so that all bottles were completely filled without air bubbles. Each of the prepared solutions was dispensed into the respective group of BOD bottles so that all bottles were completely filled without air bubbles. The bottles were closed and incubated in the dark. The biodegradation was measured by following the course of the oxygen decrease in the bottles with a special funnel. This funnel fitted exactly in the BOD bottle. Subsequently, the oxygen electrode was inserted in the BOD bottle to measure the oxygen concentration. The medium dissipated by the electrode was collected in the funnel. After withdrawal of the oxygen electrode the medium collected flo wed back into the BOD bottle, followed by removal of the funnel and closing of the BOD bottle (van Ginkel and Stroo 1992).
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- >= 13 - <= 66
- Sampling time:
- 28 d
- Remarks on result:
- other: range of results, difference vary based on chlorination
- Parameter:
- % degradation (O2 consumption)
- Value:
- >= 19 - <= 71
- Sampling time:
- 42 d
- Remarks on result:
- other: results vary depending on level of chlorination
- Parameter:
- % degradation (O2 consumption)
- Value:
- >= 21 - <= 74
- Sampling time:
- 56 d
- Remarks on result:
- other: results vary depending of level of chlorination
- Details on results:
- See full results in attached report.
Applicant's summary and conclusion
- Conclusions:
- Biodegradability is retarded by increasing degree of chlorination, though biodegradation continued for even the highest chlorinated test material in an extended study.
- Executive summary:
Biodegradability is retarded by increasing degree of chlorination, though biodegradation continued for even the highest chlorinated test material in an extended study.
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