Succinic acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / chromosome aberration study in mammalian cells

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

before 1984

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: No adequate test system.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1984

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Primary root tips of the structurally reconstructed karyotype ACB of Vicia faba were used. They were treated with succinic acid under the following conditions: 1 h treatment, 4 x 10-3 M. Additionally, a 2 h treatment with 1 x 10-3 M hydrazine was performed. Chromosome aberration frequencies were determined.

GLP compliance:

not specified

Type of assay:

other: clastogenic effects on primary root tips of Vicia faba were investigated

Test material

Test material information

Constituent 1

Reference substance name:

Succinic acid

EC Number:

203-740-4

EC Name:

Succinic acid

Cas Number:

110-15-6

Molecular formula:

C4H6O4

IUPAC Name:

succinic acid

Method

Details on test system and experimental conditions:

Primary root tips (approximately 3 cm in length) of the structurally reconstructed karyotype ACB of Vicia faba (cf. Michaelis and Rieger, 1971) were used. They were treated with succinic acid under the following conditions: 1 h treatment, 4 x 10-3 M. Additionally, a 2 h treatment with 1 x 10-3 M hydrazine was performed. After treatment and recovery in running tap water (recovery times: 21 h, 24 h) the roots were exposed to 0.05% colchicine for 2 h, fixed in ethanol/glacial acetic acid (3:1), and hydrolyzed (11 min at 60°C in HCI). Permanent Feulgen squashes were made by the dry-ice method. Aberration frequencies were determined by scoring the following types of chromatid aberrations in metaphases of first post treatment mitoses: isochromatid breaks, duplication deletions, intercalary deletions, and reciprocal chromatid translocations. Gaps were omitted. The localization of chromatid aberrations was based on a subdivision of the haploid chromosome complement into 28 segments.

Results and discussion

Additional information on results:

Succinic acid proved incapable of inducing aberrations. The aberration yield was 0.02 aberrations/cell, a frequency corresponding to the spontaneous level.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Succinic acid proved under the given conditions incapable of inducing aberrations. The aberration yield was 0.02 aberrations/cell, a frequency corresponding to the spontaneous level.

Executive summary:

Primary root tips (approximately 3 cm in length) of the structurally reconstructed karyotype ACB of Vicia faba (cf. Michaelis and Rieger, 1971) were used. They were treated with succinic acid under the following conditions: 1 h treatment, 4 x 10 -3 M. Additionally, a 2 h treatment with 1 x 10 - 3 M hydrazine was performed. After treatment and recovery in running tap water (recovery times: 21 h, 24 h) the roots were exposed to 0.05% colchicine for 2 h, fixed in ethanol/glacial acetic acid (3:1), and hydrolysed (11 min at 60°C in HCI). Permanent Feulgen squashes were made by the dry-ice method. Aberration frequencies were determined by scoring the following types of chromatid aberrations in metaphases of first post treatment mitoses: isochromatid breaks, duplication deletions, intercalary deletions, and reciprocal chromatid translocations. Gaps were omitted. The localization of chromatid aberrations was based on a subdivision of the haploid chromosome complement into 28 segments.

Succinic acid proved under the given conditions incapable of inducing aberrations. The aberration yield was 0.02 aberrations/cell, a frequency corresponding to the spontaneous level.