Registration Dossier
Registration Dossier
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EC number: 231-449-2 | CAS number: 7558-80-7
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- A comparison of the relative toxicity of bone meal and other P sources used as remedial treatments to the earthworm
- Author:
- Atuah L & Hodson ME
- Year:
- 2 011
- Bibliographic source:
- Pedobiologica - International Journal of Soil Biology
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- no
Test material
- Reference substance name:
- Potassium dihydrogenorthophosphate
- EC Number:
- 231-913-4
- EC Name:
- Potassium dihydrogenorthophosphate
- Cas Number:
- 7778-77-0
- Molecular formula:
- H3O4P.2K
- IUPAC Name:
- potassium dihydrogen phosphate
- Details on test material:
- - Name of test material (as cited in study report): potassium hydrogen phosphate (KH2PO4)
Constituent 1
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- Controls: unamended soil
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworm
- Source: Blades biological Ltd, Cowden, Edenbridge, Kent, UK
- Age at test initiation (mean and range, SD): no data
- Weight at test initiation (mean and range, SD): 340 +/- 3.7 mg
ACCLIMATION
- Acclimation period: no data
Study design
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- no
- Total exposure duration:
- 28 d
Test conditions
- Test temperature:
- 20°C
- pH:
- 7-7.2
- Moisture:
- 60% water holding capacity
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): no data
- Amount of soil or substrate: 500 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 8
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Jetting Loam - bought
- Soil texture (if natural soil): sieved to less than 2mm
Properties are similar to the OECD standard soil.
OTHER TEST CONDITIONS
- Photoperiod: performed in dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8 - Nominal and measured concentrations:
- 15,000 mg P/kg with concentrations decreasing by a factor of 1.8 downwards to 441 mg P/kg
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 3 500 other: mg P/ kg
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 28 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 3 500 other: mg P / kg
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- LC50 (14 day and 28 day values) of potassium dihydrogen phosphate was found to be > 3500 mg P / kg.
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