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Toxicological information

Additional toxicological data

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Administrative data

additional toxicological information
Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Follows basic scientific principles. Not a toxicological study, but provides supporting data to suggest the inorganic phosphates tested are tolerated in the diets of mammals.

Data source

Phosphorus Partitioning During Early Lactation in Dairy Cows Fed Diets Varying in Phosphorus Content
Knowlton KF and Herbein JH
Bibliographic source:
J. Dairy Sci. 85:1227–1236

Materials and methods

Type of study / information:
Information on the use of phosphates as food additives for animals.
Test guideline
no guideline followed
Principles of method if other than guideline:
The effect of dietary P content on P partitioning and excretion during early lactation was evaluated in 13 cows fed diets containing 0.34 (no supplementary P), 0.51, or 0.67% P. All cows were fed a common prepartum total mixed ration (TMR) (0.28% P), followed by common TMR (0.51% P) for 7 d post-partum. On day 7, cows were randomly assigned to one of the three dietary P treatments. All treatment diets contained 16.6% CP, 15.2% ADF, 26.3% NDF, and 0.74% Ca. Total collections of milk, urine, and feces were conducted during weeks 3, 5, 7, 9, and 11 of lactation.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium dihydrogenorthophosphate
EC Number:
EC Name:
Sodium dihydrogenorthophosphate
Cas Number:
Molecular formula:
sodium dihydrogen phosphate
Constituent 2
Chemical structure
Reference substance name:
Calcium hydrogenorthophosphate
EC Number:
EC Name:
Calcium hydrogenorthophosphate
Cas Number:
Molecular formula:
calcium hydrogen phosphate
Details on test material:
- Name of test material (as cited in study report): monosodium phosphate and dicalcium phosphate

Results and discussion

Applicant's summary and conclusion

Average body weight (591 kg), milk yield (47.9 kg/d), and DMI (25.2 kg/d) throughout the 10-wk trial were not affected by dietary P content. With increasing dietary P, however, linear increases in P intake (84.7, 135.2, and 161.5 g/d), fecal P (42.3, 87.5, and 108.6 g/d), urinary P (0.32, 1.28, and 3.90 g/d), and total P excretion (42.6, 88.8, and 112.5 g/d) were observed. Apparent P digestibility (49.0, 34.4, and 32.8% of P intake) decreased quadratically with increasing dietary P. Phosphorus balance was highly variable, but cows fed the 0.34% P diet were in negative P balance longer than were cows fed diets containing 0.51 or 0.67% P. With increasing dietary P, serum concentrations of inorganic P increased linearly, but serum Ca and Mg concentrations decreased. Increasing dietary P increased fecal and urinary P excretion in early lactation cows. Increased duration of negative P balance and changes in blood mineral concentrations suggest that cows fed low P diets mobilized more P from body reserves than cows on medium- and high-
P diets.