Registration Dossier
Registration Dossier
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EC number: 233-162-8 | CAS number: 10049-04-4
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- no data
- Reliability:
- 2 (reliable with restrictions)
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Effects of chlorine dioxide on neurobehavioral development of rats. In: Jolley RL, Bull RJ and Davis WP (eds) Water chlorination: Chemistry, environmental impact and health effects.
- Author:
- Taylor DH and Pfohl RJ
- Year:
- 1 985
- Bibliographic source:
- Lewis Publishers, Inc. Chelsea, MI, Vol 5: 355-364
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Pups were exposed from postnatal day 5 to 20
- GLP compliance:
- not specified
- Type of method:
- in vivo
Test material
- Reference substance name:
- 10047-04-4
- IUPAC Name:
- 10047-04-4
- Reference substance name:
- Chlorine dioxide
- EC Number:
- 233-162-8
- EC Name:
- Chlorine dioxide
- Cas Number:
- 10049-04-4
- Molecular formula:
- ClO2
- IUPAC Name:
- Chlorine Dioxide
- Details on test material:
- no data
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: new litter (5 days old)
No other data
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Details on exposure:
- no data
- Analytical verification of doses or concentrations:
- not specified
- Details on analytical verification of doses or concentrations:
- no data
- Duration of treatment / exposure:
- from 5d through 20d of age
- Frequency of treatment:
- daily
- Duration of test:
- until postnatal day 60
Doses / concentrations
- Remarks:
- Doses / Concentrations:
14 mg/kg/day
Basis:
actual ingested
- No. of animals per sex per dose:
- 8 males per litter (from unexposed dams)
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- Locomotor activity was performed.
On day 11 or 21 post partum, some rats were decapitated. Cerebellum (minus the paraflocculi) and forebrain (minus olfactory lobes) were removed and weighed and DNA was extracted.
The behaviour of 28 and 60 day old rats was examined in an open field apparatus during a 15 minute sampling period. - Statistics:
- no data
Results and discussion
Effect levels
- Dose descriptor:
- LOAEL
- Effect level:
- 14 mg/kg bw/day
- Based on:
- not specified
- Sex:
- male
- Basis for effect level:
- other: decreased activity, decreased brain weight and DNA content
Observed effects
*Pup body weight: significantly decreased at PND 21
* Exploratory behavior measured: decreased on PND 60, but not on PND 28
* Locomotor activity measured from PND 5 to 20 (measurement per litter) and from PND 50 to 60 (individual measurement): decrease on PND 18-19 and contradictory results at PND 50-60.
* DNA contents: the total DNA contents of both the cerebellum and forebrain were significantly reduced
These data indicate that rat pups exposed to ClO2 post-natally from day 5 to day 20 of age, exhibited behavioural deficits and depressed brain growth, consistent with effects produced by depressed thyroid function.
Any other information on results incl. tables
No other information
Applicant's summary and conclusion
- Conclusions:
- LOAEL was determined to be 14 mg/kg bw/day.
- Executive summary:
Chlorine dioxide was administered to Spague-Dawley pups rats by oral gavage at dose level of 14 mg/kg bw/day during postnatal day 5 through 20.
Only male pups (8 per litter) were examined for selected neurodevelopmental endpoints.
Pup body weight was significantly decreased at PND 21 by direct exposure. Exploratory behavior measured on PND 28 and 60 showed decreased in both way of exposure.
Locomotor activity measured from PND 5 to 20 (measurement per litter) and from PND 50 to 60 (individual measurement) showed a decrease on PND 18-19 and contradictory results at PND 50-60.
The total DNA content of the cerebella was significantly reduced for both the cerebellum and forebrain.
These data indicate that rat pups exposed to ClO2 both exhibited behavioral deficits and depressed brain growth, consistent with effects produced by depressed thyroid function.
LOAEL was determined to be 14 mg/kg bw/day.
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