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Ecotoxicological information

Toxicity to terrestrial arthropods

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toxicity to terrestrial arthropods: long-term
Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference Type:
secondary source
Report date:

Materials and methods

Test guideline
according to guideline
other: Test for Measuring Survival and Reproduction of Springtails Exposed to Contaminants in Soil. Report EPS Second Draft – August 2005. Method Development and Applications Section, Environmental Technology Centre, Environment Canada, Ottawa, Ontario.
Principles of method if other than guideline:
Study conducted according to Environment Canada test method.
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Reference substance 001

Sampling and analysis

Analytical monitoring:

Test substrate


Test organisms

Test organisms (species):
Folsomia candida
Animal group:
Collembola (soil-dwelling springtail)

Study design

Study type:
laboratory study
Limit test:
Total exposure duration:
28 d
Chronic Screening

Test conditions

Test temperature:
20 ± 2°C
pH (if soil or dung study):
Soil pH ranged from 6.19 to 7.64 for the beginning of all tests and from 3.92 to 7.40 for the end of all tests.
Soil moisture content of all treatments in all tests was independent of DEG concentrations and ranged from 59 to116% of the soil water holding capacity (WHC) for the beginning of all tests and from 44 to 91% of the soil WHC for the end of the invertebrate tests.
Photoperiod and lighting:
16 light : 8 dark; Fluorescent, 400-800 Lux
Details on test conditions:
The artificial soil was formulated in the Stantec laboratory from natural ingredients of silica sand (70%), kaolinite clay (20%), and Sphagnum sp. peat (10%) and it was buffered to a neutral pH range (6.0 to 7.5) with calcium carbonate.
Test unit (chamber): Glass 125-mL mason jar
Amount of soil: 30 g wet wt.
Number of replicate test units per treatment: 3
Number of organisms per test unit: 10
Nominal and measured concentrations:
0, 200, 400, 600, 800, 1600, 3200, 6400, 12800, 25600, 51200 mg/kg
The recovery of DEG from the beginning of the range-finding tests was good and ranged from 75 to 90%. DEG recovery on Day 0 of the northern
wheatgrass test was similar among all but the lowest (200 mg/kg) treatments and ranged from 85 to 102%. The recovery in the lowest treatment was below the detection limit. There was evidence of loss of DEG from soil over time; measurable concentrations of DEG were only detected in the highest concentration (25600 mg/kg) at Days 7 and 21 of the test. In addition, the % DEG recovery in these concentrations also decreased over time with 102, 74 and 63% recovery at Days 0, 7 and 21, respectively.

Results and discussion

Effect concentrationsopen allclose all
28 d
Dose descriptor:
Effect conc.:
15 689 mg/kg soil dw
Basis for effect:
28 d
Dose descriptor:
other: IC50
Effect conc.:
7 508 mg/kg soil dw
Basis for effect:
number of progeny
Details on results:
Adult Folsomia candida survival was affected adversely following exposure to DEG in AS with a chronic LC50 of 15,689 mg/kg. Chronic reproduction appeared to follow a concentrationdependent pattern and the IC50 for number of progeny produced was 7508 mg/kg.
Adult survival, no. progeny produced

Applicant's summary and conclusion