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EC number: 941-717-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-05-09 to 2000-05-11
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Valid guideline study under GLP, limited level of detail but sufficient for assessment
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
- Reference Type:
- secondary source
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- yes
- Remarks:
- Test conducted under closed-bottle conditions, i.e. “Karlsruher Flaschen”
- GLP compliance:
- yes
- Remarks:
- as specified in Annex I of Law on Hazardous Substances (German Chemicals Law - according to the respective valid issue)
Test material
- Reference substance name:
- L-menthan-3-one
- EC Number:
- 237-926-1
- EC Name:
- L-menthan-3-one
- Cas Number:
- 14073-97-3
- Molecular formula:
- C10H18O
- IUPAC Name:
- 2-isopropyl-5-methylcyclohexanone, (2S,5R)-
- Details on test material:
- - Name of test material (as cited in study report): Menthone L/Isomenthone D
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All test concentrations plus a control
- Sampling method: At test start and after 48 hours of exposure
- Sample storage conditions before analysis: Shipment for TOC determination at Dr. Dullau, Institute of Environmental Analysis, Germany
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
To produce the test concentrations, the test substance were weighed into water, treated for 60 seconds at 8000 rpm with an ultra turrax and afterwards stirred for 24 hours with a magnetic stirrer. To remove undissolved particles the resulting suspensions were filtered using folded filters of the pore size 7-12 µm.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Federal Health Office (BGA) in Berlin, Germany (laboratory bred of the parthenogenetic females at Bayer AG Leverkusen)
- Age at study initiation: 0 - 24 hours
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 15.5 ° dH
- Test temperature:
- 20.2, 20.0, 20.0, 20.1, 20.1 and 20.1 °C were measured in the control and the 6.3, 12.5, 25, 50 and 100 mg/L treatments, respectively
- pH:
- 7.9, 7.8, 7.8, 7.8, 7.8 and 7.8 were measured in the control and the 6.3, 12.5, 25, 50 and 100 mg/L treatments, respectively
- Dissolved oxygen:
- 8.9, 7.9, 8.0, 8.0, 7.8 and 7.8 mg/L were measured in the control and the 6.3, 12.5, 25, 50 and 100 mg/L treatments, respectively
- Nominal and measured concentrations:
- Nominal: 0, 6.3, 12.5, 25, 50 and 100 mg/L
Measured at 0 hours: <2, 5, 9, 14, 27 and 61 mg TOC/L, which equals to <2.6, 6.5, 11.7, 18.2, 35.1 and 79.3 mg/L of the test item
Measured at 48 hours: <2, 5, 9, 13, 26 and 62 mg TOC/L, which equals to <2.6, 6.5, 11.7, 16.9, 33.8 and 80.6 mg/L of the test item
In consequence the test results were assigned to the measured concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: so called “Karlsruher bottles” with stoppers
- Type: Closed
- Fill volume: 250 to 300 mL with head space
- Aeration: Not ventilated
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-Medium according to Elendt and BGA (1992)
- Culture medium different from test medium: No, M4-Medium according to Elendt and BGA (1992) was used for culture and dilution
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/8 hours dark
EFFECT PARAMETERS MEASURED
Oxygen [mg/L], pH and water temperature [°C] after 48 hours and the number of immobile daphnids after 24 hours and 48 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 (thus below the maximum of 2.2 according to the guidance) - Reference substance (positive control):
- no
- Remarks:
- historical control data of the laboratory used
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 30.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Calculated as geometric mean of EC0 and EC100
- Details on results:
- 24 h LC values on the basis of nominal concentrations calculated by Probit analysis were:
EC05=13 mg/L, EC10=18 mg/L, EC50=55 mg/L, EC90=170 mg/L and EC95=230 mg/L - Reported statistics and error estimates:
- The effect concentratios were determined by Probit analysis (see picture below). The correlation coefficient was 0.965.
Any other information on results incl. tables
Results after 24 and 48 hours
Nominal test item concentrations [mg/L] | Immobile daphnids, cumulated | |
after 24 h | after 48 h | |
Control | 0 | 0 |
6.3 | 0 | 0 |
12.5 | 0 | 0 |
25 | 5 | 11 |
50 | 5 | 14 |
100 | 17 | 20 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 h EC50 to daphids is 30.6 mg/L.
- Executive summary:
The effects of the test item L-menthan-3-one to daphnids (Daphnia magna) were investigated according to the EU Method C.2 (1992) under GLP conditions. The test organisms were exposed during 48 hours under static conditions in closed vessels to nominal concentrations of 0, 6.3, 12.5, 25, 50 and 100 mg/L. The dose was verified as Total Organic Carbon (TOC analysis) and found sufficiently constant under the test conditions. As the recovery was below 80 % the effects were assigned to measured concentrations.
The daphnid 48 h LC50 was calculated to be 30.6 mg/L.
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