Sodium 6-[(4-aminophenyl)azo]-5-hydroxynaphthalene-1-sulphonate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

other: read across from similar substance

Adequacy of study:

key study

Study period:

07/06/2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Real Decreto 363/1995 (BOE núm. 133 de Junio de 1995)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

7-amino-4-hydroxy-3-[[4-[(4-sulphophenyl)azo]phenyl]azo]naphthalene-2-sulphonic acid, compound with 2,2',2''-nitrilotriethanol (1:2)

EC Number:

265-016-4

EC Name:

7-amino-4-hydroxy-3-[[4-[(4-sulphophenyl)azo]phenyl]azo]naphthalene-2-sulphonic acid, compound with 2,2',2''-nitrilotriethanol (1:2)

Cas Number:

64683-40-5

Molecular formula:

C22H17N5O7S2.2C6H15NO3

IUPAC Name:

7-amino-4-hydroxy-3-({4-[(4-sulfophenyl)diazenyl]phenyl}diazenyl)naphthalene-2-sulfonic acid - 2,2',2''-nitrilotriethanol (1:2)

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution has been prepared with a concentration of 2.857 g/L of the test material. Therefore, 2.857 g of the test material have been dissolved in 1000 mL of water in a volumetric flask, Class A 1000 mL by stirring and resulting on a stock solution of 1000 mg/L. Because the test material contains 35% of active ingredient, the stock solution is of a concentration of 1000 mg/L of active material.
The different solutions used in the test have been prepared from this stock solution of 1000 mg/L active material.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

(Teleostei, Cyprinidae) (Hamilton-Buchanan) (pez cebra)
Procedencia: P & S, S.L
- size (cm): 2.86 ± 0,18
- weight (g): 0.29 ± 0,12
- Maintenance conditions:
o Temperature: 22 ºC ± 2
o Photoperiod: 12 hours of ligth / 12 hours of darkness
o Concentration of dissolved oxigen:> 60%
o Feeding:
· Feed type: Commercial flakes for fish feeding
· Frequency of feeding: 3 times/week, until 24 hours before the start of the test
· Acclimatization: minimum 12 days
· Mortality: 0%

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

120 mg CaCO3/L

Test temperature:

22 ºC ± 2

pH:

8,11

Dissolved oxygen:

> 70%

Nominal and measured concentrations:

nominal: 0,1 mg/L, 1 mg/L, 10 mg/L y 100 mg/L active material, plus one control (only water)
measured: 0,10 mg/L, 1,00 mg/L, 10,00 mg/L y 100,04 mg/L active material

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Number of died fishes during the test

conc (mg/L)
	0h	24h	48h	72h	96h
control	0	0	0	0	0
0.1	0	0	0	0	0
1	0	0	0	0	0
10	0	0	0	0	0
100	0	0	0	0	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test material does not produces any mortality in Brachydario rerio at the concentration of 100 mg/L of active material.