Sodium 6-[(4-aminophenyl)azo]-5-hydroxynaphthalene-1-sulphonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: read across from similar substance

Adequacy of study:

key study

Study period:

2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Real Decreto 363/1995 (BOE núm. 133 de Junio de 1995)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Disodium 7-amino-4-hydroxy-3-[[4-[(4-sulphonatophenyl)azo]phenyl]azo]naphthalene-2-sulphonate

EC Number:

228-589-1

EC Name:

Disodium 7-amino-4-hydroxy-3-[[4-[(4-sulphonatophenyl)azo]phenyl]azo]naphthalene-2-sulphonate

Cas Number:

6300-50-1

Molecular formula:

C22H17N5O7S2.2Na

IUPAC Name:

disodium 7-amino-4-hydroxy-3-({4-[(4-sulfonatophenyl)diazenyl]phenyl}diazenyl)naphthalene-2-sulfonate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

Taking into account the value of 20% of active ingredient, the study will be carried out with the 100% active ingredient of the presented material.
Preparation of the 1 g / L of the test material : 1 g of the test material has been dissolved in 100 mL of culture medium in a volumetric flask, Class A of 100 mL by means of agitation and a stock solution of 1000 mg / L has been obtained. The test material has a content of 20% active material, thus the stock solution contains 200 mg / L of active material. The different solutions used in the test have been prepared from this stock solution of 1000 mg / L.
Actual concentration of the stock solution: 1007 mg/L of active ingredient.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Age: ≤ 24-hours life, in good physiological conditions
Origin: Stirling University, United Kingdom

Culture conditions: The cultivation of daphnia from which the Individuals to carry out the test has been done with the following conditions:
- Temperature: between 18 and 22 ºC
- Photoperiod of 16 hours of light / 8 hours of darkness
- No aeration
- Feeding: Type of food: Chlorella (freshwater microalgae) and marine algae extract
·-Frequency of feeding: 3 times / week

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 ± 2 ºC

pH:

average value pH at the beginning of the test: 7,62 (interval 7,66-7,81)
average value pH at the end of the test: 7,39 (interval 7,42-7,76)

Dissolved oxygen:

average value O2 at the beginning of the test: 8,52 mg/L (interval 8,52-8,49 mg/L)
average value O2 at the end of the test: 8,41 mg/L (interval 8,39-8,3

Nominal and measured concentrations:

Nominal: 1 mg/L, 2,2 mg/L, 4,8 mg/L, 10 mg/L, 22 mg/L, 48 mg/L, 100 mg/L, 220 mg/L, 480 mg/L and 1000 mg/L
Measured: 1,01 mg/L, 2,22 mg/L, 4,83 mg/L, 10,07 mg/L, 22,15 mg/L, 48,35 mg/L, 100,7 mg/L, 221,5 mg/L, 483,4 mg/L and 1007 mg/L.

Details on test conditions:

Photoperiod: darkness
Test vessels: Test tubes (40 mL) capped with parafilm.
Test volume used: 10 mL
Number of test vessels per concentration: 4
Number of individuals per concentration: 20, divided into 5 individuals per test vessel
Age of individuals: less than 24 hours of life

Composition of culture medium OECD 202:
- Solution 1: CaCl 2 2H 2 O 11.76 g / L H 2 O
- Solution 2: MgSO 4 7H 2 O 4.93 g / L H 2 O
- Solution 3: NaHCO 3 2.59 g / L H 2 O
- Solution 4: KCl 0.23 g / L H 2 O
Add to 1000 mL of deionized water:
Solution 1 25 mL
Solution 2 25 mL
Solution 3 25 mL
Solution 4 25 mL

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Effect concentrations 4

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Results with reference substance (positive control):

The EC50-24h of potassium dichromate is between 0.6 mg / L and 1.7 mg / L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

LC50 > 200 mg/l

Executive summary:

The result obtained in this test indicates that the test substance is not toxic to Daphnia magna at the concentration of 200 mg / L of active material.