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Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Published, investigative, non-guideline, non-GLP study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
no guideline followed
Principles of method if other than guideline:
A chronic toxicity study was performed with the aquatic midge Tanytarsus dissimilis to determine the effects on growth and reproduction in natural lake water with pH adjustment using sulphuric acid.
GLP compliance:
not specified
Remarks:
older, published study
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Analytical monitoring:
yes
Details on sampling:
Not applicable
Vehicle:
no
Details on test solutions:
Test media were prepared using carbon-filtered Lake Superior water. The pH was adjusted using sulphuric acid
Test organisms (species):
other: Tanytarsus dissimilis
Details on test organisms:
The species was selected for use because of its widespread distribution, value as food for fish, ease of rearing in the laboratory and its relatively short life cycle.
Test type:
static
Water media type:
freshwater
Limit test:
no
Remarks on exposure duration:
Three generations in the control
Post exposure observation period:
Not applicable
Hardness:
Test water data (after 35 days), all values in mg/L:
pH 3.0 - 78.0, pH 4.0 - 64.0, pH 5.0 - 54.0, pH 5.5 - 54.0, pH 6.0 - 50.0, pH 7.8 - 50.0
Test temperature:
Test water data (after 35 days), all values in degrees C:
pH 3.0, 4.0, 5.0, 5.5, 6.0 and 7.8 - all were 18.5
pH:
Not applicable
Dissolved oxygen:
Test water data (after 35 days), all values in mg/L:
pH 3.0 - 7.71, pH 4.0 - 8.06, pH 5.0 - 7.93, pH 5.5 - 8.70, pH 6.0 - 8.54, pH 7.8 - 9.01
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal pH values:
pH 3.0, pH 4.0, pH 5.0, pH 5.5, pH 6.0, pH 7.8
Details on test conditions:
A series of different pH were prepared by adjusting the pH of natural, carbon- filtered lake water with sulphuric acid. The following pHs were used:

pH 3.0, 4.0, 5.0, 5.5 and 6.0. Lake water was used as a control and had a pH of 7.8.
Reference substance (positive control):
no
Dose descriptor:
NOEC
Effect conc.:
0.15 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: equivalent to pH 5.5
Details on results:
pH 3.0 - all larvae died within 5 days

pH 4.0 - all larvae died within 10 days

pH 5.0 - larvae survived, began pupating after 13 to 17 days, adults failed to emerge within 35 days. Five of the pupal cases were examined, larvae had successfully pupated and adult characteristics were present but the adults were unable to escape from the pupal case. The 10 remaining larvae were kept under test conditions for an additional 10 days and no adults appeared.

pH 5.5 - larve survived, began pupating after 12 to 14 days, adults began to emerge after 27 days, all 15 larvae emerged successfully within 35 day, eggs hatched within 35 days. The treatment was allowed to continue and three complete generations were obtained. The numbers increased from the original 15 to between 500 and 550 larvae.

pH 6.0 - larve survived, began pupating after 12 to 14 days, adults began to emerge after 27 days, all 15 larvae emerged successfully within 35 day, eggs hatched within 35 days. The treatment was allowed to continue and three complete generations were obtained. The numbers increased from the original 15 to between 500 and 550 larvae.

pH 7.8 (control) - larve survived, began pupating after 12 to 14 days, adults began to emerge after 27 days, all 15 larvae emerged successfully within 35 day, eggs hatched within 35 days. The treatment was allowed to continue and three complete generations were obtained. The numbers increased from the original 15 to between 500 and 550 larvae.

Based on the results an NOEC of pH 5.5 (equivalent to 0.15 mg/L) was observed.
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
not applicable

Only a brief description of the materials and methods was reported. However, the reported study details indicate that the study was conducted in a scientifically valid way and the results are considered to be reliable.

Validity criteria fulfilled:
not applicable
Remarks:
There were no appropriate study guidelines when the study was conducted.
Conclusions:
Based on the reported information an acceptable assessment of chronic toxicity to aquatic invertebrates was achieved.
Executive summary:

A chronic toxicity study with the midge Tanytarsus dissimilis was conducted. Midge larvae were exposed to a series of test media, carbon-filtered lake water with pH adjustment using sulphuric acid, to varying pHs. At pH 3.0 and 4.0 all of the larvae died within 10 days. At pH 5 larvae pupated but the adults were unable to escape from the pupal case. At pH 5.5 and 6.0 there were no effects on the initial larvae and three complete generations were obtained. The initial 15 larvae increased to between 500 and 550 larvae. The results from the control group (lake water with no adjustment pH 7.8) were the same as in the pH 5.5 and 6.0.

The no-observed effect concentration (NOEC) for T. dissimilis (based on reproduction) exposed to sulphuric acid was observed to be pH 5.5, equivalent to 0.15 mg/L.

Description of key information

A published paper on the chronic effects of sulphuric acid on a freshwater invertebrate, the midge Tanytarsus dissimilis has been summarised and a chronic NOEC obtained.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
NOEC
Effect concentration:
0.15 mg/L

Additional information

A chronic toxicity study with the midge Tanytarsus dissimilis was conducted. Midge larvae were exposed to a series of test media, carbon-filtered lake water with pH adjustment using sulphuric acid, to varying pHs. At pH 3.0 and 4.0 all of the larvae died within 10 days. At pH 5 larvae pupated but the adults were unable to escape from the pupal case. At pH 5.5 and 6.0 there were no effects on the initial larvae and three complete generations were obtained. The initial 15 larvae increased to between 500 and 550 larvae. The results from the control group (lake water with no adjustment pH 7.8) were the same as in the pH 5.5 and 6.0.

The no-observed effect concentration (NOEC) for T. dissimilis (based on reproduction) exposed to sulphuric acid was observed to be pH 5.5, equivalent to 0.15 mg/L.