4-hydroxybutan-2-one

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1987-08-10 - 1987-08-14

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure according to national standards

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German industrial standard test guideline DIN 38 412, part L15.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Hydroxyacetone

EC Number:

204-124-8

EC Name:

Hydroxyacetone

Cas Number:

116-09-6

IUPAC Name:

1-hydroxyacetone

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): Hydroxyaceton
- Physical state: Liquid
- Analytical purity: about 95%
- Other: easily soluble

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The product was added to the test water without any pretreatment ;subsequently the fish were placed into the aquaria .

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Goldorfe
- Strain: Leuciscus idus (golden variety)
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation: 5.1 cm (range: 4.8-5.7)
- Weight at study initiation: 1.8 g (range: 1.4-2.7)
- Feeding during test: no
- Frequency: Withdrawal of food before exposure: 1 day

ACCLIMATION

- Acclimation to test water and test temperature: 3 days

- Type and amount of food: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Feeding frequency: ad libitum.

HOUSING
- Housing water: The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air .
- Aeration: oilfree air
- Light regime: 16 : 8 hours light : dark
- Hardness: 2.5 mM
- Oxygen concentration: > 60 % of maximum saturation
- pH: about 7.6
- Water temperature: 20 +-1°C
- Duration of housing: about 2 weeks
- Medical treatment: Twice with 0.05 mg/L Malachitegreen and once with 10 mg/L tetracyclinhydrochlorid
- Feeding: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Amount of feed: ad libitum
- Mortality during the last 2 weeks of housing: 0.1 %

Study design

Test type:

static

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L

Test temperature:

20°C +-1

pH:

6.5-7.8

Dissolved oxygen:

7.6-8.8 mg/L

Nominal and measured concentrations:

nominal: 0, 1000, 2150, 4640, 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: All-glass aquarium (30 CM X 22 CM X 24 CM)
- Material, size, headspace, fill volume: 10 Liters
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1.8 (g fish / L test water)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Ca/Mg ratio: 4 : 1
- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light:dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms

TEST CONCENTRATIONS
- Spacing factor for test concentrations: about 2.2
- Reasons for the selection of the concentrations : based on the results of a range finding study.

Reference substance (positive control):

yes

Remarks:

Chloroacetamid

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

4 640 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4 640 - 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

- LC50: ca. 32 mg/L (48 h)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes