4-hydroxybutan-2-one

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015-03-23 - 2015-03-25

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study according to OECD guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-hydroxybutan-2-one

EC Number:

209-693-6

EC Name:

4-hydroxybutan-2-one

Cas Number:

590-90-9

Molecular formula:

C4H8O2

IUPAC Name:

4-hydroxybutan-2-one

Constituent 2

Reference substance name:

2-Butanone, 4-hydroxy-

IUPAC Name:

2-Butanone, 4-hydroxy-

Details on test material:

- Name of test material (as cited in study report): 4-Hydroxybutan-2-one
- Physical state: colourless or light yellow liquid
- Lot/batch No.:10781727
- Expiration date of the lot/batch: November 04, 2016
- Other: miscible with water at room temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: one limit concentration 100 mg/L plus the control
- Sample storage conditions before analysis: The sample of the test item was stored at ambient temperature without exposure to light in a tightly
sealed container.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test item in the amount of 100.70 mg was weighed in a glass crystallizer transferred by multiple washing with Elendt M7 medium into a glass flask, the total volume was filled up to 1007 mL. The test item concentration of 100 mg/L was visually homogeneous
and transparent. After 24 h of exposure the test item concentration and the control were renewed to
freshly prepared.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology
- Age at study initiation (mean and range, SD): 24 hours old (not first brood progeny)
- Method of breeding: Daphnia magna was cultured in glass beakers with a capacity of 150 mL (one parent per vessel)
containing 100 mL of Elend M7 medium, at room temperature ranging from 18 to 22°C with daily cycle
16 h light : 8 h dark
- Feeding during test: no feeding


ACCLIMATION

- Type and amount of food: suspension of algae; mixture of two species Pseudokirchneriella subcapitata :
Desmodesmus subspicatus (in 2:1 ratio) Group B vitamins and micronutrients necessary for proper growth
were supplied with the lyophilized suspension of Spirulina sp.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

209 mg Ca CO3/L

Test temperature:

20.4 - 21.9 ºC

pH:

7.31 - 7.46

Dissolved oxygen:

8.65 - 9.01 mg/L

Nominal and measured concentrations:

nominal test item concentrations: 100 mg/L plus the control

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers of 150 mL with transparent lids
- Type (delete if not applicable): closed
- Aeration: no aeration.
- Renewal rate of test solution (frequency/flow rate): one renewal
- No. of organisms per vessel: five Daphnia magna in each replicate
- No. of vessels per concentration (replicates): 8
- No. of vessels per control (replicates): 8


TEST MEDIUM / WATER PARAMETERS
- The Elendt M7 medium recommended by the OECD Guideline No. 202 (2004) was used to culture the
test organism and as a diluent/solvent of the test item

OTHER TEST CONDITIONS

- Light intensity:daily cycle 16 h light : 8 h dark; fluorescent light source

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Daphnia magna was observed for immobilization after 24 and 48 h of exposure. The Daphnia magna
was considered immobile if they showed no ability to swim within 15 seconds after swirling the test
vessel

TEST CONCENTRATIONS

- Test concentrations: were determined on the basis of the preliminary tests results

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50 (24h): 0.64 mg/L
EC50 (48h): 0.57 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In the definitive test the validity criteria were met according to OECD Guideline No. 202 (2004):
- The percentage of immobilization of Daphnia magna in the control was 5% (criterion: not more
than 10%),
- The dissolved oxygen concentrations in the test vessels were within the range of
8.62 – 9.01 mg/L (criterion: not less than 3 mg/L)