Diethyl phthalate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / chromosome aberration study in mammalian cells

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

publication

Title:

Chromosome tests with 134 compounds on chinese hamster cells in vitro. a screening for chemical carcinogens

Author:

M. Ishidate, S. Odashima

Year:

1977

Bibliographic source:

Elsevier/North-Holland Biomedical Press

Materials and methods

Principles of method if other than guideline:

Three different doses, including the 50% inhibition dose of each agent were prepared and separately added to 3-day-old cultures. Chromosome preparations were made, as a rule at 24 and 48 h, or when necessary at 6 h, after the treatment. Cells were treated with colcemid (0.2 µg/ml) for 2 h, and after trypsinization, they were incubated in 0.075 M KCl hypotonic solution for 15 min at 37°C. The cells were fixed with ice-cold fixative (methanol : glacial acetic acid, 3 : 1 v/v) which was changed 3 times. A few drops of the suspension were then placed on clean dry slides which were held horizontally under an electric heater. The slides were stained with 1% Giemsa's buffered solution (pH 6.8) for 20 min.

GLP compliance:

not specified

Type of assay:

in vitro mammalian chromosome aberration test

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl phthalate

EC Number:

201-550-6

EC Name:

Diethyl phthalate

Cas Number:

84-66-2

Molecular formula:

C12H14O4

IUPAC Name:

diethyl phthalate

Method

Species / strain

Species / strain / cell type:

Chinese hamster lung fibroblasts (V79)

Additional strain / cell type characteristics:

not applicable

Metabolic activation:

with and without

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Remarks:

albumin bovine

True negative controls:

no

Positive controls:

yes

Remarks:

Solvent (albumin bovine) was used as control next to untreated cells.

Results and discussion

Test results

Species / strain:

Chinese hamster lung fibroblasts (V79)

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative