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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
4-[(1E,3S)-3-ethenyl-3,7-dimethylocta-1,6-dienyl]phenol
EC Number:
685-515-4
Cas Number:
10309-37-2
Molecular formula:
C18H24O
IUPAC Name:
4-[(1E,3S)-3-ethenyl-3,7-dimethylocta-1,6-dienyl]phenol

Test solutions

Vehicle:
yes
Details on test solutions:
Vehicle control: Tween 80

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
22.4-22.9
pH:
7.89-8.54
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 mL conical f1asks. The test containers were incubated at 21 to 24℃ with a continuous light intensity of 4440 to 8880 Lux.
- fill volume: 150 mL
- Initial cells density: 10000 cells/ml. Algal cells were counted at every 24 hours interval up to the end of the treatment period.
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 6
OTHER TEST CONDITIONS
- Light intensity: 7310-7640 Lux

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1, 2.5, 5 mg/L
- Results used to determine the conditions for the definitive study: 5 mg/L (limit of solubility)
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 2.108 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
> 2.108 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
As the percent change in analysed concentration over nominal concentration at the end of test was > 20 %, the results were expressed as initial measured concentration (i.e. 2.1 085 mg/L).
Reported statistics and error estimates:
Statistical analysis was not carried out as there was no inhibition of growth at the limit concentration tested.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes