Registration Dossier
Registration Dossier
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EC number: 228-726-5 | CAS number: 6337-43-5
Bioaccumulation: aquatic / sediment
Administrative data
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study according to OECD guideline and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Tetraethyl 2,2'-(1,4-phenylenedimethylidyne)bismalonate
- EC Number:
- 228-726-5
- EC Name:
- Tetraethyl 2,2'-(1,4-phenylenedimethylidyne)bismalonate
- Cas Number:
- 6337-43-5
- Molecular formula:
- C22H26O8
- IUPAC Name:
- 1,3-diethyl 2-({4-[3-ethoxy-2-(ethoxycarbonyl)-3-oxoprop-1-en-1-yl]phenyl}methylidene)propanedioate
Constituent 1
- Radiolabelling:
- no
Sampling and analysis
- Details on sampling:
Frequency ofanalysis
(1) Test water analysis
The test water of each level was analyzed once before first analysis of test fish and at the same time as analysis of test fish thereafter. The number of each sample was one.
(2) Test fish analysis
The test fish ofLevel 1 and 2 was analyzed five times in duration of exposure. Four fish were taken out at each sampling time and divided into two groups, then both were analyzed individually*3.
The control fish was analyzed before the experimental starting and after the experimental completion. Four fish were taken out at each sampling time and divided into two groups, and then each was analyzed individually. In addition, six fish were separately taken out and three groups (two fish per group) were used for measurement of lipid contents.
*3 Because one fish was not enough to the analysis sensitivity for the analysis of the test item, two fish were pooled for a group.
Test solutions
- Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
The test item and HCO- 40 (50 times amount of the test item) were dissolved in N,N-dimethylformamide with supersonic wave irradiation to prepare a stock solution.
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Length 6.9 - 8.5 cm
Study design
- Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
Test conditions
- Hardness:
- Total hardness (Ca, Mg) mg/L 13.2
- Test temperature:
- Level 1 24.2 - 24.5 °C
Level 2 24.1 - 24.5 °C
Control24.0 - 24.5 °C - pH:
- Level 1 7.8 -7.9
Level 2 7.8 -7.9
Control 7.8 -7.9 - Dissolved oxygen:
- Level 1 7.8 - 8.0 mgIL
Level 2 7.8 mgIL
Control 7.4 -7.9 mgIL - Nominal and measured concentrations:
- Level 1: 2µg/L
Level 2: 0.2 µg/L - Details on estimation of bioconcentration:
- Since the test substance concentrations in fish were below the detection limit no BCF was calculated. Therefor
Results and discussion
Bioaccumulation factoropen allclose all
- Type:
- BCF
- Value:
- <= 2
- Basis:
- whole body w.w.
- Calculation basis:
- other:
- Remarks on result:
- other: On basis of the detection limit of the substance in fish a theoretical maximum BCF was determined
- Remarks:
- Conc.in environment / dose:2 µg/L
- Type:
- BCF
- Value:
- < 19
- Basis:
- whole body w.w.
- Calculation basis:
- other:
- Remarks on result:
- other: On basis of the detection limit of the substance in fish a theoretical maximum BCF was determined
- Remarks:
- Conc.in environment / dose:0.2 µg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The maximum BCF based on detection limit of of the test item is determined to be 2.0 (test concentration 2µg/L) and 19 (test concentration 0.2 µg/l). Therefore the test item has a very low potential for bioaccumulation
- Executive summary:
The maximum BCF based on detection limit of of the test item is determined to be 2.0 (test concentration 2µg/L) and 19 (test concentration 0.2 µg/l). Therefore the test item has a very low potential for bioaccumulation
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