Triphenylphosphine oxide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16.05 - 20.05. 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study according to national standards

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: to German national standard DIN 38 412, part L15.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Triphenylphosphine oxide

EC Number:

212-338-8

EC Name:

Triphenylphosphine oxide

Cas Number:

791-28-6

Molecular formula:

C18H15OP

IUPAC Name:

triphenylphosphine oxide

Details on test material:

- Name of test material (as cited in study report): Triphenylphosphinoxid roh
- Purity: about 80 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solution was prepared by adding the test item to the test media without any pre-treatment. Subsequently the fish were placed into the test aquaria.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Strain: Leuciscus idus L. (golden variety)
- Source: Fish farm Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range and SD): 7.8 cm (range: 6.6 - 9.1 cm)
- Weight at study initiation (mean and range, SD): 4.7 g (range: 3.1 - 6.7 g)
- Method of breeding: Culture conditions: oil-free aerated and charcoal filtered tap water, flow-through system
Duration of housing: about 6 months
Mortality during the last 2 weeks of housing: 0.9 %
Medical treatment: Twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride


ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: Growing feed F/B 50,
- Feeding frequency: ad libitum
- Mortality during the acclimation period: 0 %
Withdrawal of food: 1 day before and during exposure.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

20 ± 1 °C

pH:

about 8.0

Dissolved oxygen:

7.1 - 8.8 mg/l

Nominal and measured concentrations:

Nominal concentrations: 0, 10, 21.5, 46.4, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (30 x 22 x 24 cm)
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 4.7 g fish/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982; prepared from fully demineralised tap water by adding:
294.0 mg/l CaCl2 * 2 H2O
123.3 mg/l MgSO4 + 7 H2O
63.0 mg/l NaHCO3
5.5 mg/l KCl
- Continuous aeration with oil-free air

Test water ready for use:
Total hardness: 2.5 mmol/l
Acid capacity: 0.8 mmol/l
Ratio Ca/Mg ions: 4 : 1
Ratio Na/K ions: 10 : 1
pH: about 8.0

- Intervals of water quality measurement: temperature, pH and oxygen content after 0, 24, 48, 72 and 96 h

OTHER TEST CONDITIONS
- Photoperiod: : 16 : 8 hours day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 1, 4, 24, 48, 72 and 96 h.
Endpoints investigated: mortality and symptoms of intoxication.

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

46 - 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

21.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

21.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

- Results with reference substance valid? yes
- LC50 (48h): 32 mg/L

Any other information on results incl. tables

Sublethal observations / clinical signs:

Undissolved test substance was visible on the bottom increasing with increase in the concentration.