Esterification product of castor oil and tetrahydromethyl-1,3-isobenzofuranedione

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline study and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Esterification product of castor oil and tetrahydromethyl-1,3-isobenzofuranedione

EC Number:

700-064-6

Cas Number:

2105830-60-0

Molecular formula:

Not applicable as this is a UVCB substance

IUPAC Name:

Esterification product of castor oil and tetrahydromethyl-1,3-isobenzofuranedione

Constituent 2

Reference substance name:

[TN]Multiester P97-463[/TN][SPEC][/SPEC][AM]100 %[/AM]

IUPAC Name:

[TN]Multiester P97-463[/TN][SPEC][/SPEC][AM]100 %[/AM]

Details on test material:

Pseudokirchneriella subcapitata, Fresh Water Algal Growth Inhibition Test with
Multiester P97-463.
The study procedures described in this report were based on the OECD guideline No. 201,
2006. In addition, the procedures were designed to meet the test methods of the EEC directive
92t69, Part C.3, 1992, the ISO International Standard 8692, 2004 and the OECD series on
testing and assessment number 23, 2000.
The batch of Multiester P97-463 tested was a very viscous amber liquid and not completely
soluble in test medium at the loading rate tested.
Preparation of the test solution started with a 1: 1 stock in acetone. 260 µl (-200 mg) of this
stock was added to 1 litre of M2 medium resulting in a nominal concentration of 100 mg/l. After
a 3-day stirring period and a 10-minute stabilisation period, the aqueous mixture (containing test
substance particles) was filtered through a membrane filter (0.45 µm). The clear and colourless
filtrate was used for testing.
A limit test was performed exposing exponentially growing algae to a solvent-control, a blankcontrol
and the filtered solution prepared at a loading rate of 100 mg/l. The initial cell density
was 10 x E04 cells/ml and each test group contained 6 replicates. The total test period was 72 hours.
The project did not include sampling for determination of actual exposure concentrations.
The study met the acceptability criteria prescribed by the protocol and was considered valid.
Multiester P97-463 did not reduce growth rate or inhibit yield in a filtered solution prepared at a
loading rate of 100 mg/l after 72 hours of exposure (NOEC). Hence, the 72h-EC50 for both
growth rate reduction and yield inhibition was beyond the maximum soluble concentration.

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

other: growth rate & biomass

Effect concentrations 2

Duration:

72 h

Dose descriptor:

other: EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

other: growth rate & biomass

Remarks on result:

other: Not toxic at loadings significantly above the solubility limit

Any other information on results incl. tables

Pseudokirchneriella subcapitata, Fresh Water Algal Growth Inhibition Test with

Multiester P97-463.

The study procedures described in this report were based on the OECD guideline No. 201,

2006. In addition, the procedures were designed to meet the test methods of the EEC directive

92t69, Part C.3, 1992, the ISO International Standard 8692, 2004 and the OECD series on

testing and assessment number 23, 2000.

The batch of Multiester P97-463 tested was a very viscous amber liquid and not completely

soluble in test medium at the loading rate tested.

Preparation of the test solution started with a 1: 1 stock in acetone. 260 µl (-200 mg) of this

stock was added to 1 litre of M2 medium resulting in a nominal concentration of 100 mg/l. After

a 3-day stirring period and a 10-minute stabilisation period, the aqueous mixture (containing test

substance particles) was filtered through a membrane filter (0.45 µm). The clear and colourless

filtrate was used for testing.

A limit test was performed exposing exponentially growing algae to a solvent-control, a blankcontrol

and the filtered solution prepared at a loading rate of 100 mg/l. The initial cell density

was 10 x E04 cells/ml and each test group contained 6 replicates. The total test period was 72 hours.

The project did not include sampling for determination of actual exposure concentrations.

The study met the acceptability criteria prescribed by the protocol and was considered valid.

Multiester P97-463 did not reduce growth rate or inhibit yield in a filtered solution prepared at a

loading rate of 100 mg/l after 72 hours of exposure (NOEC). Hence, the 72h-EC50 for both

growth rate reduction and yield inhibition was beyond the maximum soluble concentration.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present study with Pseudokirchnerie/la subcapitata,
Multiester P97-463 did not reduce growth rate or inhibit yield in a filtered solution prepared at a
loading rate of 100 mg/l after 72 hours of exposure (NOEC). Hence, the 72h-EC5o for both
growth rate reduction and yield inhibition was beyond the maximum soluble concentration.

Executive summary:

SUMMARY

Pseudokirchneriella subcapitata, Fresh Water Algal Growth Inhibition Test with

Multiester P97-463.

The study procedures described in this report were based on the OECD guideline No. 201,

2006. In addition, the procedures were designed to meet the test methods of the EEC directive

92t69, Part C.3, 1992, the ISO International Standard 8692, 2004 and the OECD series on

testing and assessment number 23, 2000.

The batch of Multiester P97-463 tested was a very viscous amber liquid and not completely

soluble in test medium at the loading rate tested.

Preparation of the test solution started with a 1: 1 stock in acetone. 260 µl (-200 mg) of this

stock was added to 1 litre of M2 medium resulting in a nominal concentration of 100 mg/l. After

a 3-day stirring period and a 10-minute stabilisation period, the aqueous mixture (containing test

substance particles) was filtered through a membrane filter (0.45 µm). The clear and colourless

filtrate was used for testing.

A limit test was performed exposing exponentially growing algae to a solvent-control, a blankcontrol

and the filtered solution prepared at a loading rate of 100 mg/l. The initial cell density

was 10 x E04 cells/ml and each test group contained 6 replicates. The total test period was 72 hours.

The project did not include sampling for determination of actual exposure concentrations.

The study met the acceptability criteria prescribed by the protocol and was considered valid.

Multiester P97-463 did not reduce growth rate or inhibit yield in a filtered solution prepared at a

loading rate of 100 mg/l after 72 hours of exposure (NOEC). Hence, the 72h-EC50 for both

growth rate reduction and yield inhibition was beyond the maximum soluble concentration.