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EC number: 225-833-9 | CAS number: 5107-67-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Start of experiment: June 19, 1990 ; End of experiment: June 22, 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-oxocyclohexane-1,1,3,3-tetrapropionic acid
- EC Number:
- 225-833-9
- EC Name:
- 2-oxocyclohexane-1,1,3,3-tetrapropionic acid
- Cas Number:
- 5107-67-5
- Molecular formula:
- C18H26O9
- IUPAC Name:
- 3-[1,3,3-tris(2-carboxyethyl)-2-oxocyclohexyl]propanoic acid
- Test material form:
- not specified
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- The albino rabbit is the recommended species for skin irritation/corrosion studies.
- Animal strain: New Zaeland white rabbits (Chbb:NZW)
- Acclimatisation period: At least 5 days.
Group size and husbandry:
The test was performed on 3 male rabbits, chcked for normal skin conditions, weighing between 2240 to 2490 g (approx. 9-13 weeks old). The animals were housed individually in metal cages, identified by ear tattoo, kept at a constant room temperature of 20 +/- 3°C, at a relative humidity of 30-70% and on 12 hours light cycle day.
The rabbits receibed ad libitum standard rabbit pellet-Nafag N°. 814, Gossau, Switzerland and fresh water. All batches of the diet are assayed for nutritive ingredients and contaminant level by the manufacturer.
The drinking water quality fulfilled the critical parameters in the specifications of the "Schweizerisches Lebensmittelbuch" (Edition 1972)
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- shaved
- Vehicle:
- other: Patches were moistened before application with 0.5% (w/v) carboxymethylcellulose in 0.1% (w/v) aqueous polysorbate 80.
- Controls:
- other: Control gauze patch was applied to the contralateral flank of each rabbits.
- Amount / concentration applied:
- 0.5 g. of the test item was applied.
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 72 hours
- Number of animals:
- 3 male rabbits
- Details on study design:
- An area of at least 36 cm2 was shaved on both flanks of the animals approximately 24 hours before treatment.
A gauze patch (Aprrox. 12-16 cm2) bearing 0.5 g of the test article was applied to the right flank of each animal. A control gauze patch was applied to the contralateral flank. Both patches were moistened before application with 0.5% (w/v) carboxymethylcellulose in 0.1% (w/v) aqueous polysorbate 80.
The patches were loosely covered with an aluminium foil (approx. 36 cm2) and held in place for 4 hours by an adhesive tape (Isoplast, Isoplast AG, CH-5200 Brugg).
SCORING SYSTEM:
Erythema and eschar formation.
- No erythema: 0
- Very slight erythema (barely perceptible): 1
- Well defined erythema: 2
- Moderate to severe erythema: 3
- Severe erythema (beet redness) to slight eschar formation (injuries in depth): 4
Total possible erythema score: 4
Edema formation
- No edema: 0
- Very slight edema (barely perceptible): 1
- Slight edema (edges of area well defined by definite raising): 2
- Moderate edema (raised approximately 1 mm): 3
- Severe edema (raised more than 1 mm and extending beyond area of exposure ): 4
Total possible edema score: 4
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Remarks:
- 3 male rabbits
- Time point:
- other: Mean 24-72 hours
- Score:
- 0
- Remarks on result:
- other: 2 male rabbits showed a score of 1 for erythema one hour after removing the bandages, after 24 hours they are fully recovered.
- Irritation parameter:
- edema score
- Basis:
- mean
- Remarks:
- mean 3 male rabbits
- Time point:
- other: Mean 24-72 hours
- Score:
- 0
- Irritant / corrosive response data:
- Because no reactions were obsreved at 24 hours to 72 hours after removing the bandages, the test was ended after the 72 hours evaluation.
- Other effects:
- No data
Any other information on results incl. tables
Individual skin reaction scores:
Animal n° | ERYTHEMA | EDEMA | ||||
085/M | 205/M | 107/M | 085/M | 205/M | 107/M | |
CF/TF | CF/TF | CF/TF | CF/TF | CF/TF | CF/TF | |
after 1 hr. | 0/1 | 0/0 | 0/1 | 0/0 | 0/0 | 0/0 |
after 24 hrs. | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 |
after 48 hrs. | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 |
after 72 hrs. | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 |
mean 4 -72 hrs. | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 |
CF= control flank
TF= Test flank
M= Male
F= Female
Body weight (g)
animal n° | 085/M | 205/M | 107/M |
at start if test | 2490 | 2260 | 2240 |
after 3 days (end) | 2570 | 2330 | 2300 |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The test item is classified as non-irritant to the skin rabbit.
- Executive summary:
The acute skin irritation/corrosion study in albino rabbits was performed to determine the irritant or corrosive potency of the test substance on the albino rabbit skin.
This test was besed on the OECD Guideline N°.404, adopted May 12, 1981.
Under the experimental conditions employed, the test item, induced slight erythema reactions when applied to the albino rabbits skin.
The reactions were observed only one hour after removing the bandages. Because the mean values of the recordings 24 to 72 hours after application are scored zero, the test item can be classified as non-irritant.
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