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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The 48 h EC50 was 27 mg/L with 95 % confidence limits of 23 - 31 mg/L.
The No Observed Effect Concentrations after 48 hours exposure was 18 mg/L respectively. The Lowest Observed Effect Concentrations after 48 hours exposure was 32 mg/L respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
27 mg/L

Additional information

A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphniasp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008. Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at nominal concentrations of 10, 18, 32, 56 and 100 mg/L for 48 hours at a temperature of approximately 21°C under semi-static test conditions. The number of immobilized daphnia were recorded after 24 and 48 hours.


With the exception of the 10 mg/L fresh test preparation at 24 hours, analysis of the fresh test preparations at 0 and 24 hours showed measured test concentrations to be near nominal. There was no significant change in the measured concentrations in the old media at 24 and 48 hours and so the results are based on nominal test concentrations only.

Exposure of Daphnia magna to the test item gave a 48 h EC50 of 27 mg/L with 95% confidence limits of 23 - 31 mg/L. The NOEC was 18 mg/L and the LOEC was 32 mg/L. The results from the positive control with potassium dichromate were within the normal range for this reference item.