Diphenyl sulphide

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Data is from peer-reviewed journal

Data source

Reference

Reference Type:

publication

Title:

Salmonella mutagenicity tests: IV. Results from the testing of 300 chemicals.

Author:

Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K.

Year:

1988

Bibliographic source:

Environ Mol Mutagen. 1988;11 Suppl 12:1-157.

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Bacterial reverse gene mutation assay was performed to evaluate the mutagenic potential of the test material allyl propyl disulfide (CAS No. 2179-59-1).

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Allyl propyl disulphide

EC Number:

218-550-7

EC Name:

Allyl propyl disulphide

Cas Number:

2179-59-1

Molecular formula:

C6H12S2

IUPAC Name:

Allyl propyl disulphide

Test material form:

liquid

Specific details on test material used for the study:

Name of test material (as cited in study report): Allyl propyl disulphide
Molecular formula:C6H12S2
Molecular weight:148.2928 g/mol
Substance Type: Organic
Physical State: Liquid

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA97, TA98, TA100, TA102, TA1535, TA1537

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

liver S-9 from Aroclor-induced male Sprague-Dawley rats and Syrian hamsters

Test concentrations with justification for top dose:

Up to 333 microgram/plate

Vehicle / solvent:

No data available

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

not specified

Positive control substance:

not specified

Details on test system and experimental conditions:

METHOD OF APPLICATION: preincubation

Rationale for test conditions:

No data available

Evaluation criteria:

No data available

Statistics:

No data available

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA97, TA98, TA100, TA102, TA1535, TA1537

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Remarks on result:

other: no mutagenic potential

Applicant's summary and conclusion

Conclusions:

In an Ames test using Salmonella typhimurium strains TA97, TA98, TA100, TA102, TA1535, TA1537 with and without S9 activation, doses up to 333 microgram/plate allyl propyl disulfide (CAS No. 2179-59-1) were not mutagenic.

Executive summary:

The bacterial reverse gene mutation assay was performed to evaluate the mutagenic potential of the test material allyl propyl disulfide (CAS No. 2179-59-1). The chemical was tested for mutagenicity, in Salmonella typhimurium, using a preincubation protocol.The test was performed in the absence of exogenous metabolic activation, and in the presence of liver S-9 from Aroclor-induced male Sprague-Dawley rats and Syrian hamsters. In an Ames test using Salmonella typhimurium strains TA97, TA98, TA100, TA102, TA1535, TA1537 with and without S9 activation, doses up to 333 microg/plate allyl propyl disulfide (CAS No. 2179-59-1) were not mutagenic. Hence, according to CLP criteria, it can be concluded that allyl propyl disulfide (CAS No. 2179-59-1) was non genotoxic.