Sodium 4-vinylbenzenesulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

January 4, 1999 to March 30, 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
None specified in the study report.

Analytical monitoring:

yes

Details on sampling:

Samples of 10 ml test solution were taken from each prepared solution at the start of test and each test vessel after 48 hours. The samples were analyzed with HPLC. When analysis of test solution was conducted, measurement using standard solution was done for each test sample analysis and the concentration was determined with peak height of the spectrum.

Vehicle:

yes

Details on test solutions:

1000 mg of sodium p-toluenesulfonate was added to 1L measuring flask and then diluted to 1L with diluting water. Thus obtained solution was used for the test.

Test organisms (species):

Daphnia magna

Details on test organisms:

Young daphnids, aged less than 24 hours at the start of the test, were used for the test.
These animals originated from National Institute of Environment Study were cultivated in this Laboratory.
Breeding method to prepare young daphnia for the test.
Visually healthy and enough size adult females, which are brood progeny were selected from stock cultivated animals and transfer them to another vessels. In the next day, new born animals were moved to other vessels. These young animals have been bred for 2 or 3 weeks under following conditions to use as parental animals for the test animal.
Young animals were removed at least twice a week from females which were matured and began to deliver offspring. After from 2 to 4 weeks, just before date of start of exposure, brood females in the brood chamber were selected and next day (within 24 hours), delivered young animals were used for the test. However, daphnia from batches, where many dead animals, resting-eggs or male offspring were observed were not used.
Breeding water: Diluting water
Breeding population: 15 Daphnids /L-breeding water
Breeding temperature: 20±1°C
Lighting: Room light 16 hour-light/8 hour-dark
Feed: Chlorella vulgaris
Amount of feed: 0.15mgC (organic carbon content) of Chlorella vulgaris per a daphnia manga per day.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

No post exposure observation period specified in the study report.

Hardness:

26mg/L (CaC03 converted)

Test temperature:

Water temperature during 48 hours exposure was 20.2 to 20.9 °C.

pH:

pH during exposure was 7. 7 to 7.9.

Dissolved oxygen:

Dissolved Oxygen Concentrations during exposure test were from 8.6 to 8.8 mg/L

Salinity:

None specified.

Nominal and measured concentrations:

At the start of exposure and after 48 hours, concentrations of test solutions were analyzed. Concentration of test sample at the start of exposure was 1040 mg/L (Nominal concentration 1000mg/L), and after 48 hours from exposure the concentration was 1070 mg/L. The ratio to the nominal concentration was from 104 to 107%.

Details on test conditions:

Test conditions
1) Exposure method: Static
2) Exposure period: 48 hours
3) Repeat number: 4 groups per one concentration
4) The number of animals: 20 animals /concentration (5 animals/group; 20 animals/concentration level)
5) Volume of test solution: 100 mL
6) Water temperature: 20 ± 1°C
7) Lighting: room light 16 hour-light/8 hour-dark
8) Feed: None

Diluting water
Dechlorinated water [Industrial water (drinking water grade treated with chlorine) in the Nishiki plant, Kureha Corporation, is treated with activated carbon to remove residual chlorine and then aeration with air] was used for the test. Major water qualities of diluting water were that hardness was 26mg/L (CaC03 converted) and pH was 7.8.

Test equipment, test vessels and isothermal bath
Test vessel: 100ml Beaker
Isothermal bath: Isothermal room
Water thermometer: Mercury thermometer
pH meter: Toa Electronics (HM-30V)
DOC meter: Iijima Electronic (B-505)

Determination of test concentration
Based on preliminary test results, 1000 mg/L and control were set.

Test procedure
On the previous day of start of exposure, all young daphnids continuous cultivated for 2 to 4 weeks were withdrawn from vessels.
On the start day of exposure young animals aged less than 25 hours were moved to other vessels and used for the test.
After measured pH, DOC and water temperature of each test solution, 5 of water fleas were added into the test vessel.
After exposure started, the number of immobilized animals was record at 24 h and 48 h.

Reference substance (positive control):

yes

Remarks:

potassium dichromate; special grade

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Environment factors which affect reliability of the test results
None

Test material concentration in the test aqueous solution.
At the start of exposure and after 48 hours, concentrations of test solutions were analyzed. Concentration of test sample at the start of exposure was 1040 mg/L (Nominal concentration 1000mg/L), and after 48 hours from exposure the concentration was 1070 mg/L. The ratio to the nominal concentration was from 104 to 107%.

Water temperature, Dissolved oxygen concentration and pH of test solution
Water temperature during 48 hours exposure was 20.2 to 20.9 °C. pH during exposure was 7. 7 to 7.9. Dissolved Oxygen Concentrations during exposure test were from 8.6 to 8.8 mg/L and beyond 60% of saturated dissolved oxygen concentration.
(Saturated dissolved oxygen concentration at 20.0 °C: 8.84 mg/L)

Results with reference substance (positive control):

Result of the immobilization test to the standard substance (potassium dichromate; special grade) was EiC5o 0.079 mg/L-48 h.

Reported statistics and error estimates:

None specified in the study report.

Measured Concentration of p-toluenesulfonic acid sodium salt

During a 48-Hour Exposure of Daphnia magna under Static Test Condition

Nominal Concentration mg/L	Measured Concentration (mg/L)		Percent of Nominal
	0 Hour New	48 Hour Old	0 Hour New	48 Hour Old
Control	N.D.	N.D.	-	-
1000	1040	1070	104	107

New: freshly prepared test solution

Old: test solution after 48 hours exposure period

 

Mortality or Immobility of Daphnia magna Exposed to

p-toluenesulfonic acid sodium salt under Static Test Conditions

Nominal Concentration mg/L	Cumulative Number of Dead or Immobilized Daphnia magna (Percent Mortality or Immobility)
	24 Hour	48 Hour
Control	0	0
1000	0	0

 

Calculated EiC50 for Daphnia magna Exposed to p-toluenesulfonic

acid sodium salt Based on Nominal Concentrations under Static Test Conditions

Exposed Period (Hour)	EiC50 (mg/L)	95% Confidence Limit (mg/L)	Statistical Method
24	>1000	-	-
48	>1000	-	-

 

Observation of No Observed Effect Concentration (NOECi)

and Lowest Concentration in 100% Mortality or Immobility Values

Exposed Period (Hour)	No Observed Effect Concentration (NOECi) (mg/L)	Lowest Concentration in 100% Mortality or Immobility (mg/L)
24	1000	-
48	1000	-

 

pH During a 48-Hour Static Exposure of Daphnia magna

to p-toluenesulfonic acid sodium salt

Nominal Concentration (mg/L)	pH
	0 Hour New	48 Hour Old
Control	7.8	7.9
1000	7.7	7.8

New: freshly prepared test solutions

Old: test solutions after 48 hours exposure period

 

Dissolved Oxygen Concentrations During a 48-Hour

Static Exposure of Daphnia magna to p-toluenesulfonic acid sodium salt

Nominal Concentration (mg/L)	Dissolved Oxygen Concentration (mg/L)
	0 Hour New	48 Hour Old
Control	8.7	8.6
1000	8.8	8.7

New: freshly prepared test solutions

Old: test solutions after 48 hours exposure period

 

Temperature During a 48-Hour Static Exposure of

Daphnia magna to p-toluenesulfonic acid sodium salt

Nominal Concentration (mg/L)	Temperature (°C)
	0 Hour New	48 Hour Old
Control	20.9	20.5
1000	20.6	20.2

New: freshly prepared test solutions

Old: test solutions after 48 hours exposure period

Validity criteria fulfilled:

not specified

Conclusions:

No Observed Effective Concentration (NOEiC): 1000 mg/L
The lowest concentration producing 100% immobility: Not calculated

Executive summary:

Daphnia manga Immobilization test of Sodium p-toluenesulfonate

Study No.: N01998-SEI02

This study conducted according to OECD Test Guideline No.202

1) Test material: Sodium p-toluenesulfonate

2) Exposure method: Static

3)Test Organism: Water flea (Daphnia manga)

4) Exposure duration: 48 hours

5) Reputation: 4 groups per exposure level

6) Animals: 20 animals per dose (5 animals per group, 20 animals per dose)

7) Test concentration: control and 1000 mg/L

8) Test volume: 100 mL

9) Lighting: 16 hour-light and 8 hour-hour dark

10) Test temperature: 20 ± 1°C

 

Results

1) Result after 24-hour exposure

50% Immobilized concentration at 24 hours (EiC50): > 1000mg/L

2) Result after 48-hour exposure

50% Immobilized concentration at 48 hours (EiC50): > 1000mg/L

 

No Observed Effective Concentration (NOEiC): 1000 mg/L

The lowest concentration producing 100% immobility: Not calculated

Description of key information

The immobilisation of Daphnia magna was determined acording to the OECD 202 test guideline in accordance with GLP, with exposure to Daphnia magana under static conditions at a test concentration of 1000 mg/l.  As the substance is highly water soluble and hydrolytically stable no analytical monitoring was conducted.  Following a 48 hour exposure, no immobilisation occured.  The EC50 is >1000 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 000 mg/L