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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Diethyl ethylmalonate
EC Number:
205-093-3
EC Name:
Diethyl ethylmalonate
Cas Number:
133-13-1
Molecular formula:
C9H16O4
IUPAC Name:
1,3-diethyl 2-ethylpropanedioate
Test material form:
liquid
Details on test material:
Test item: diethyl ethylmalonateCAS No.: 133-13-1Physical state: colourless liquidStorage: in cool, dry, well-ventilated place

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Type of inoculum: aerobic mixed bacterial cultureSource: Municipal wastewater treatment plant, Esztergom-Kertváros
Duration of test (contact time):
28 d
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
PREPARATION OF INOCULUM TEST CULTUREBefore the test 300 ml stock culture was filtered through a filter with 0.2 mm pore size to remove the bigger insoluble particles and then the dry substance content of the filtrate was determined. The measured value was 4.01 g/L. For preparing the inoculum culture 150.01 ml mineral medium was added to 49.96 ml filtered stock culture. The calculated dry mass of inoculum culture was 1 g/L.PREPARATION OF REFERENCE SOLUTIONFor the reference solution 320.02 mg sodium acetate was diluted in 100 ml mineral medium.PREPARATION OF TEST VESSELSFollowing test vessels were prepared and analysed for the study:- Test item (in two replicates)- Blank control (in two replicates)- Reference solution (positive control)- Reference and test item solution (toxicity control)- Sterilized solution of test item (abiotic control)
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

% Degradation
Key result
Parameter:
% degradation (O2 consumption)
Value:
30
Sampling time:
28 d
Remarks on result:
other: mean of two replicates
Details on results:
The biodegradability of the test item on day 14th was 20%.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The biodegradability of diethyl ethylmalonate was investigated in Ready Biodegradability – Manometric respirometry test (according to OECD 301 F guideline). According to the OECD 301 guideline diethyl ethylmalonate is not ready biodegradable.