Benzene mono-C10-13-alkyl derivatives, distillation residues, sulfonated, sodium salts

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other:

Remarks:

Study meets the critria for Klimisch score 2 (reliable with restrictions) because it followed an internationally recognised method (OECD Guideline 305) and was carried out to Good Laboratory Practice. However, this study was only available for review as a robust study summary.

Justification for type of information:

The sulfonate group is a common functional group present in each of the analogue members, and is expected to exhibit similar biological activities with little influence from the length of carbon chain. Any potential breakdown products, via physical or biological processes, are also expected to result in structurally similar chemicals. In addition, non-random patterns have been observed for the toxicological effects (e.g., available data showed low levels of acute toxicity, lack of mutagenic potential, skin irritating properties and a trend of change in ecotoxicological potential based on molecular weight). These common behaviors and consistent trends suggest a common mechanism and mode of action thereby providing further supporting evidence for the read-across among the linear and branched alkyl benzene sulfonates.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Deviations:

not specified

GLP compliance:

yes

Radiolabelling:

yes

Vehicle:

no

Details on preparation of test solutions, spiked fish food or sediment:

Not reported

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout

Route of exposure:

aqueous

Test type:

flow-through

Test temperature:

14 ± 0.1°C

pH:

Not reported

Dissolved oxygen:

Not reported

TOC:

Not reported

Salinity:

Not reported

Conductivity:

Not reported

Details on test conditions:

Rainbow trout were exposed in groups of 52 to an aqueous solution of test material for 42 days under dynamic conditions. Samples of test fish were taken from the solvent control, 0,001 and 0.01 mg/l test groups on days 21, 25, 28, 32, 35 and 39 of the uptake phase, and concentrations of the test material in fish tissues were determined. After 42 days exposure, the remaining fish were subjected to a depuration period of 61 days. Samples of test fish were taken from the solvent control, 0.001 and 0.01 mg/l test groups on days 3, 6, 11, 13 and 61 of the depuration phase and the concentrations of the test material determined.

Nominal and measured concentrations:

Not reported

Key result

Conc. / dose:

0.001 mg/L

Type:

BCF

Value:

45 dimensionless

Basis:

other: whole body (not stated if w.w. or d.w)

Calculation basis:

steady state

Key result

Conc. / dose:

0.01 mg/L

Type:

BCF

Value:

58 dimensionless

Basis:

other: whole body (not stated if w.w. or d.w)

Calculation basis:

steady state

Key result

Conc. / dose:

0.001 mg/L

Type:

BCF

Value:

38 dimensionless

Basis:

other: whole body (not stated if w.w. or d.w)

Calculation basis:

steady state

Conc. / dose:

0.01 mg/L

Type:

BCF

Value:

64 dimensionless

Basis:

other: whole body (not stated if w.w. or d.w)

Calculation basis:

steady state

Details on results:

14-C SP6012 (oil free) was not significantly accumulated in fish tissue and was observed to be eliminated over a 61-day depuration period. At the end of the depuration period, 70 and 90% of 14C-SP6012 (oil free) was eliminated from the fish tissues at concentrations of 0.001 and 0.01 mg/l, respectively. Refer to Tables 1 and 2

Table 1. Mean measured test concentrations in whole fish during the exposure phase

Test concentration (mg/l)	Mean measured test concentration in whole fish on day 21 of exposure phase	Mean measured test concentration in whole fish on day 39 of exposure phase
0.001	0.033	0.043
0.01	0.61	0.57

Table 2. Mean measured test concentrations in whole fish during the depuration phase

Test concentration (mg/l)	Mean measured test concentration in whole fish on day 13 of depuration phase	Mean measured test concentration in whole fish on day 61 of depuration phase
0.001	0.015	0.013
0.01	0.43	0.28

Conclusions:

14-C SP6012 (oil free) was not significantly accumulated in fish tissue and was observed to be eliminated over a 61-day depuration period. At the end of the depuration period, 70 and 90% of 14C-SP6012 (oil free) was eliminated from the fish tissues at concentrations of 0.001 and 0.01 mg/l, respectively.

Executive summary:

In a biconcentration study, rainbow trout (Oncorhynchus mykiss) were exposed to 14-C SP6012 (oil free) at concentrations of 0.001 and 0.01 mg/L under dynamic conditions conditions. 14-C SP6012 (oil free) was not significantly accumulated in fish tissue and was observed to be eliminated over a 61-day depuration period.

 

This toxicity study is classified as acceptable and satisfies the guideline requirement for a fish bioaccumulation study.

 

Description of key information

In an OECD Guideline 305 study on rainbow trout, 14-C SP6012 (oil free) was not significantly accumulated in fish tissue and was observed to be eliminated over a 61-day period.

BCF= 45 Dimensionless

In a bioconcentration study performed according OECD Guideline 305 (Bioconcentration: Flow-through Fish Test), rainbow trout (Oncorhynchus mykiss) were exposed to 14-C SP6012 (oil free) at concentrations of 0.001 and 0.01 mg/L under dynamic conditions. 14-C SP6012 (oil free) was not significantly accumulated in fish tissue and was observed to be eliminated over a 61-day depuration period. The calculated BCF was 45 and 38 at 0.001 mg/l (steady state and kinetic state respectively) and 58 and 64 at 0.01 mg/l (steady state and kinetic state respectively).

This toxicity study is classified as acceptable and satisfies the guideline requirement for a fish bioaccumulation study.

Key value for chemical safety assessment

BCF (aquatic species):

45 dimensionless

Additional information

The prediction of the BCF performed with the computer program BCFBAF v3.01 (EPIWIN software) by US-EPA

yielded a value between 56.23 to 70.79 L/kg wet-wt, suggesting not a strong bioaccumulation potential.