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EC number: 943-688-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- other: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Unchanged samples
- All test medium samples for the analytical determinations were taken from the approximate center of the test vessels (after mixing the medium) and kept at-18°C to -25°C until analysis. - Details on test solutions:
- STOCK SOLUTION
A stock solution of 219.9 mg test substance was mixed in a beaker with 200 ml test water and filled up to 1000 ml with test water. This stock solution was diluted 1:10 with test water to get a final stock solution.
The test concentrations were prepared with this final stock solution.
PREPARATION AND APPLICATION OF TEST SOLUTION:
- the test medium used had the following composition (concentration in test)
NaHCO3 (65 mg/l)
MgSO4 * 7 H2O (123.3 mg/l)
KCl (5.8 mg/l)
CaCI2 * 2 H2O (294 mg/l)
Na2SiO3 * 9 H2O 4.3 (mg/l)
NaNO3 274 (μg/l)
KH2PO4 1430 (μg/l)
K2HPO4 184 (μg/l)
- the test water medium was prepared with bidistilled water
- the medium was oxygen saturated at the beginning of the test
REFERENCE SUBSTANCE: potassium dichromate - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The organism used in this test system is the species Daphnia magna Strauss. The Daphnia were bred in the laboratories of the Product Ecology under standardised conditions.
Breeding:
- Cultures of Daphnia magna are held in glass vessels containing approx. 3.0 litre of reconstituted water at 18 - 22 °C. The water is renewed partially three times per week.The Daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a Tetramin-extract in such quantites that the food is consumed within 24 hours.
Pretreatment:
- 24 hours before the start of the exposure reproductive Daphnia were separated from the young Daphnia by sieving all individuals through a 1000 μm sieve.
For each test concentration 20 Daphnia divided into two groups of 10 animals were used. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 282 mg/l CaCO3
- Test temperature:
- 21 °C
- pH:
- in a range of 7.6 - 8.0
- Dissolved oxygen:
- saturated
- Nominal and measured concentrations:
- 0.038, 0.083, 0.18, 0.40, 0.9 and 1.9 mg test article/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- a range finding test was carried out at concentration from 0.01 - 100 mg/l to determine the concentrations for the main test.
- the Daphnia were exposed to the test article in 250 ml beakers containing 60 - 100 ml test medium with 10 animals per beaker
- all tests were running in duplicate
OTHER TEST CONDITIONS
- Light conditions: 12 hours light / 12 hours dark- alkalinity: 0.8 mmol/l - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95 %confidence limit: 0.499 - 0.720 mg/l
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 0.18 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95 % confidence limit: 0.16 - 0.252 mg/l
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.038 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 0.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- In all test vessels no visible alteration of the test substance in the test medium was observed after 48 hours. During the test the actual test concentrations in the analysed test media were in the range of 89 - 108 % of the nominal values. Therefore, the reported results are related to the nominal values of the test concentrations of the test article. No immobilization and abnormal behaviour of the Daphnia was observed in the blank control.
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (48h) = 0.20 mg/l
- Executive summary:
A study was conducted to determine the acute toxicity of the test substance to Daphnia magnain a 48 h static test according to the OECD Guideline 202, in according to the GLP Principles.
Daphnia were exposed to nominal test substance concentrations of0.038, 0.083, 0.18, 0.40, 0.9 and 1.9mg/l. Analytical determination of the test substance concentration was carried out at 0 and 48 h by spectrophotometry, leading to test substance concentrations in the range of 89 - 108 % of the nominal values.
Results:
EC0 (48h) = 0.038 mg/l
EC50 (48h) = 0.20 mg/l
EC100 (48h) = 0.9 mg/l
Reference
Measurements at the end of the test:
Nominal conc. (mg/l) | blank | 0.038 | 0.083 | 0.18 | 0.40 | 0.9 | 1.9 |
oxygen (%) | 97 | 97 | 97 | 97 | 98 | 98 | 96 |
pH | 8.0 | 7.9 | 8.0 | 7.9 | 8.0 | 8.0 | 8.0 |
temperature (°C) | 21 | 21 | 21 | 21 | 21 | 21 | 21 |
Description of key information
EC50 (48h) = 0.20 mg/l
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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