N-hydroxyphthalimide

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from Scifinder

Qualifier:

according to guideline

Guideline:

other: CO2 generation amount method

Principles of method if other than guideline:

Biodegradation study was conducted according to CO2 generation amount method for evaluating the percentage biodegradability of test substance 2-hydroxy-1H-isoindole-1,3(2H)-dione.

GLP compliance:

not specified

Specific details on test material used for the study:

- IUPAC name: 2-hydroxy-1H-isoindole-1,3(2H)-dione
- Molecular formula: C8H5NO3
- Molecular weight: 163.132 g/mol
- Smiles notation: c12c(C(=O)N(C1=O)O)cccc2
- InChl: 1S/C8H5NO3/c10-7-5-3-1-2-4-6(5)8(11)9(7)12/h1-4,12H
- Substance type: Organic
- Physical state: Solid

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Duration of test (contact time):

14 d

Based on:

not specified

Parameter followed for biodegradation estimation:

CO2 evolution

Key result

Parameter:

% degradation (CO2 evolution)

Value:

95.8

Sampling time:

14 d

Remarks on result:

other: Other details not known

Details on results:

Test substance undergoes 95.80% degradation by CO2 evolution parameter in a 14 day period.

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

The final aerobic percentage degradation rate of test substance was determined to be 95.80% degradation by CO2 evolution parameter in a 14 day period. Thus, based on percentage degradation, 2-hydroxy-1H-isoindole-1,3(2H)-dione is considered to be readily biodegradable in water.

Executive summary:

Biodegradation study was conducted according to CO2 generation amount method for evaluating the percentage biodegradability of test substance 2 -hydroxy-1H-isoindole-1,3(2H)-dione (CAS no. 524 -38 -9).The final aerobic percentage degradation rate of test substance was determined to be 95.80% degradation by CO2 evolution parameter in a 14 day period. Thus, based on percentage degradation, 2 -hydroxy-1H-isoindole-1,3(2H)-dione is considered to be readily biodegradable in nature.

Description of key information

Biodegradation study was conducted according to CO2 generation amount method for evaluating the percentage biodegradability of test substance 2 -hydroxy-1H-isoindole-1,3(2H)-dione (CAS no. 524 -38 -9) (Scifinder, American Chemical Society, 2017).The final aerobic percentage degradation rate of test substance was determined to be 95.80% degradation by CO2 evolution parameter in a 14 day period. Thus, based on percentage degradation, 2 -hydroxy-1H-isoindole-1,3(2H)-dione is considered to be readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

Various study and predicted data for the target compound 2-hydroxy-1H-isoindole-1,3(2H)-dione (CAS No. 524-38-9) and various supporting studies for its read across substance were reviewed for the biodegradation end point which are summarized as below:

 

In an experimental key study, biodegradation experiment was conducted according to CO2 generation amount method for evaluating the percentage biodegradability of test substance 2 -hydroxy-1H-isoindole-1,3(2H)-dione (CAS no. 524 -38 -9) (Scifinder, American Chemical Society, 2017). The final aerobic percentage degradation rate of test substance was determined to be 95.80% degradation by CO2 evolution parameter in a 14 day period. Thus, based on percentage degradation, 2 -hydroxy-1H-isoindole-1,3(2H)-dione is considered to be readily biodegradable in nature.

 

In a supporting study from secondary source (Scifinder, American Chemical Society, 2017), biodegradation experiment was performed according to the shaking cultivation method for evaluating the percentage biodegradability of test substance 2 -hydroxy-1H-isoindole-1,3(2H)-dione (CAS no. 524 -38 -9). The primary aerobic percentage degradation rate of test substance was determined to be 93.60% degradation in a 5 day period. Thus, based on percentage degradation, 2 -hydroxy-1H-isoindole-1,3(2H)-dione is considered to be readily biodegradable in nature.

 

For target chemical 2-hydroxy-1H-isoindole-1,3(2H)-dione(CAS No. 524-38-9), biodegradability of2-hydroxy-1H-isoindole-1,3(2H)-dioneis predicted using OECD QSAR toolbox version 3.4 with logKow as the primary descriptor (2017).Test substance undergoes 73.63% degradation by BOD in 28 days. Thus, based on percentage degradation, the test chemical 2-hydroxy-1H-iso indole-1,3(2H)-dione was estimated to be readily biodegradable in water.

 

In a supporting study from authoritative database (J-CHECK, 2016), biodegradation experiment was conducted for 14 days for evaluating the percentage biodegradability of read across substance1H-isoindole-1,3(2H)-dione(CAS no. 85 -41 -6). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of read across substance was determined to be 92, 94 and 100% by BOD(NH3), TOC removal and HPLC parameter in 14 days. Thus, based on percentage degradation, 1H-isoindole-1,3(2H)-dione is considered to be readily biodegradable in nature.

 

For another read across chemical 2 -bentzofuran-1,3 -dione (CAS no. 85 -44 -9),biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of read across substance 2 -bentzofuran-1,3 –dione(J-CHECK, 2016). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of read across substance was determined to be 85.2, 93 and 96.4% degradation by BOD, TOC removal and UV-Vis parameter in 14 days. Thus, based on percentage degradation, 2 -bentzofuran-1,3 -dione is considered to be readily biodegradable in nature.

 

Another biodegradation study for the same read across substance 2 -bentzofuran-1,3 -dione (CAS no. 85 -44 -9) was conducted for 30 days for evaluating the percentage biodegradability of substance 2 -bentzofuran-1,3 -dione(HSDB and OECD SIDS, 2016). The study was performed according to OECD Guideline 301 D “Ready Biodegradability: Closed Bottle Test”. Concentration of inoculum i.e, sludge used was 3 mg/l and initial test substance conc. used in the study was 1000 mg/l, respectively. The percentage degradation of read across substance was determined to be 45, 58 and 71% by BOD parameter after 5, 10 and 30 days, respectively. Thus, based on percentage degradation, 2 -bentzofuran-1,3 -dione is considered to be readily biodegradable in nature.

 

On the basis of above results for target chemical2-hydroxy-1H-isoindole-1,3(2H)-dione(from secondary source Scifinderand OECD QSAR toolbox version 3.4, 2017) and for its read across substance (from authoritative database J-CHECK, HSDB and secondary source OECD SIDS, 2016), it can be concluded that the test substance2-hydroxy-1H-isoindole-1,3(2H)-dionecan be expected to be readily biodegradable in nature.