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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4-dichloro-2-(1,1,2,3,3,3-hexafluoropropoxy)-5-nitrobenzene
EC Number:
620-459-6
Cas Number:
130841-23-5
Molecular formula:
C9H3Cl2F6NO3
IUPAC Name:
1,4-dichloro-2-(1,1,2,3,3,3-hexafluoropropoxy)-5-nitrobenzene
Details on test material:
- Name of test material (as cited in study report): CA 946 A
- Batch No.: P.906005
- Purity: 96,9 %
- Received: 1993-12-15
- Storage: room temperatur
- Stability: 07/96

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
(see Attachment / Table 3)
- Concentrations mg/l: 0,29 / 0,59 / 1,44 / 3,67 / 3,54
- Sampling method: HPLC
- Sample storage conditions before analysis: -18°C

Composition samples of each test concenttraion were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at -18 to -22°C until analysis.

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Strain: Straus 1820
- Source: Ciba-Geigy Ltd., testing facility
- Age at study initiation: above 24 h
- Breeding:
- Cultures of Daphnia are maintained in glass vessels containing approx 2,5 l of reconstituted water
- Temperature: 20 °C +/- 1
- water is renewed partially thrice weekly
- Feeding at each water renewal: suspension of green algea (Scenedesmus subspicatus) supplemented by a suspension of Tetramin-extract
- Feeding during the test: none
- No. of Daphnia: 20 per concentration and control. 4 replicates of 5 daphnia each

PRETREATMENT/QUARANTINE
- 24 h before the begin of the exposure reproductive daphnia are separated from the young by sieving all individuals through a 800 µm sieve.
This operation is repeated immediately before the exposure and the young (0-24h of age) are reteined for the test.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
Immobilizations were recorded after 24 and 48 hours exposure (Attachment 1)

Test conditions

Test temperature:
20 °C (+/- 1)
pH:
7,9
Nominal and measured concentrations:
The oxygen content, pH and temperature were measured at 0 and 48 hours (Attachment 2)

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.59 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95%

Applicant's summary and conclusion

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