Cadmium nitrate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

not applicable

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Reliable with restriction. Not according to GLP nor to specific test guidelines.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

publication

Title:

The mutagenic activity of 61 agents as determined by the micronucleus, Salmonella, and sperm abnormality assays.

Author:

Bruce WR and Heddle JA

Year:

1979

Bibliographic source:

Can J Genet Cytol. 21(3):319-34.

Materials and methods

Principles of method if other than guideline:

The Ames Salmonella test was used to test cadmium chloride for reversion of his- auxotrophs of Salmonella Thyphimurium in the strains TA1535, TA1537, TA98 and TA100 which were obtained from Dr. B. Ames

GLP compliance:

not specified

Type of assay:

bacterial gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Cadmium chloride

EC Number:

233-296-7

EC Name:

Cadmium chloride

Cas Number:

10108-64-2

IUPAC Name:

cadmium dichloride

Details on test material:

name of test substance: cadmium chloride

Method

Target gene:

not applicable

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Details on mammalian cell type (if applicable):

not applicable

Additional strain / cell type characteristics:

other: each strain was irradiated at 10; 100; 1000; 10,000; 100,000 without S9

Metabolic activation:

with and without

Metabolic activation system:

S9

Test concentrations with justification for top dose:

0,05; 0,5; 5; 50; 500 µg/plate

Vehicle / solvent:

H2O

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

no

Positive control substance:

no

Remarks:

none

Details on test system and experimental conditions:

plate method of Ames (plate incorporation)
endpoint: gene mutation

Evaluation criteria:

no information

Statistics:

no statistics reported

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Remarks:

in vitro gene mutation

Cytotoxicity / choice of top concentrations:

no cytotoxicity nor precipitates, but tested up to recommended limit concentrations

Vehicle controls validity:

not valid

Untreated negative controls validity:

valid

Positive controls validity:

not specified

Additional information on results:

none

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

none

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

This study reported that cadmium chloride was negative in Salmonella typhimurium with and without S9 tested from 0.05 - 500μg.

Executive summary:

Bruce and Heddle (1979) reported that cadmium chloride was negative in Salmonella typhimurium with and without S9 tested from 0.05 - 500μg.