Reaction mass of Fatty acids, montan-wax and Fatty acids, montan-wax, calcium salts and Montan wax

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

From APR 18 2002 to MAY 22 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to relevant guideline and compliant to GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Montan waxes, type WE 4

IUPAC Name:

Montan waxes, type WE 4

Constituent 2

Reference substance name:

Reaction mass of Fatty acids, montan-wax and Glycerides, montan-wax and Montan wax

EC Number:

914-469-2

Molecular formula:

not applicable

IUPAC Name:

Reaction mass of Fatty acids, montan-wax and Glycerides, montan-wax and Montan wax

Details on test material:

"Licolub WE 4"
active content: 100 %

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Secondary effluent of domestic sewage treatment plant. Filtrate of activated sludge tank.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

4.46 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

All measurements were performed in duplicate:

Dilution water: deionized (distillate) water
Mineral medium: Mix of different minerals and deionized water - stock solutions:
(a) 8.5 g of KH2PO4 , 21.75g of K2HPO4 , 33.4 g of Na2HPO4*2H20 and 0.5 g NH4CI made up to 1 L;
(b) 36.4 g of CaCl2*2H20 made up to 1 L
(c) 22.5 g of MgSO4*7H20 made up to 1 L
(d) 0.25 g of FeCI3*H2O made up to 1 L
Preparation: Adding 1 mL of solutions (a), (b), (c) and (d) to 800 mL dilution water, this was made up to 1 L .
Apparatus: BOD bottles, Oxygen electrode and meter, large glass bottles, graduated cylinder, thermometer, pipette;

Procedure:
a) Preparation of bottles
The preparation of the stock solution was set up by a weighted portion of about 40 mg from the test substance which was made up to 100 ml with distillate water (stock solution).
The mineral medium was strongly aerated for at least 30 minutes and was allowed to stand for 20 h at the test temperature (02-concentration was about 9 mg/L at 20 deg C).
Then there was added about 1.5 L fully-aerated mineral medium to a large bottle (4 L) so that it was about one-third full. Afterwards there was added an appropriate amount (about 40 mL) of the stock solution of the test substance to reach the final concentration of 4 mg/L (between 2 and 5) and inoculated with secondary effluent. Finally, it was made up the solution to final volume of 4L with aerated mineral medium.
The same procedure was repeated to prepare the flasks for the reference compound.
Each test series consisted of:
- about 10 bottles containing test suspension (test substance and inoculum)
- about 10 bottles containing inoculum alone (blank inoculum)
- about 10 bottles containing reference compound and inoculum (procedure control)

b) Test procedure
Afterwards the prepared solution was dispensed immediately into the BOD bottles by hose from the lower quarter of the appropriate large bottles, so that all BOD bottles were completely filled. After analyzing the zero-time bottles for dissolved oxygen the other ones were incubated in dark at constant temperature (about 20 °C).
To allow the assessment of percentage removal in a 14 d (10 d) window observations of dissolved oxygen were carried out at days 1, 2, 5, 11, 15, 25 and 28.
All test series were running in parallel. The reference substance was propylenalkylbenzolsulfonate with a known biological degradation rate (about 30 % in Zahn-Wellens test for inherent biodegradability according to OECD 302B).

Reference substance

Reference substance:

other: propylene alkylbenzene sulfonate, cross-linked

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

59.7

Sampling time:

28 d

Remarks on result:

other: 10 respectively 14 day-window not fulfilled

Details on results:

ThOD submission substance: 1.28 mg O2/mg;
ThOD reference substance: 2.84 mg O2/mg;
See table section "Any other information on results incl. tables"!

BOD5 / COD results

Results with reference substance:

See table section "Any other information on results incl. tables"!

Any other information on results incl. tables

ThDO test substance: 1.28 mg O2/mg

ThDO reference substance: 2.84 mg O2/mg

Days	Dissolved oxygen			Nitrate	Nitrite
	Inoculum	Test item	Reference item	Test item	Test item
d	mg/L	mg/L	mg/L	mg/L	mg/L
1	7.99	7.71	7.93	<0.2	<0.01
2	7.68	7.62	7.54	<0.2	<0.01
5	7.54	7.23	6.86	<0.2	<0.01
12	7.38	6.60	6.12	<0.2	<0.01
17	7.23	4.59	5.76	<0.2	<0.01
23	7.12	3.88	5.32	<0.2	<0.01
28	6.98	3.37	5.12	0.2	<0.01

Specific biological oxygen depletion (BOD)

	oxygen depeltion (abs.)	specific oxygen depeltion (under consideration of nitrification	specific oxygen depeltion (BOD)	specific oxygen depeltion (BOD)
	test substance	test substance	test substance	reference substance
d	mg/mg	mg/mg	mg/mg	mg/mg
1	0.06	0.06	0.013	0.040
2	0.06	0.06	0.013	0.077
5	0.31	0.31	0.070	0.372
12	0.79	0.79	0.176	0.689
17	2.64	2.64	0.592	0.803
23	3.24	3.24	0.726	0.984
28	3.61	3.40	0.763	1.016

Biological degradation (BOD/ThOD x 100%; under consideration of nitrification):

	specific oxygen depeltion (BOD)	specific oxygen depeltion (BOD)	biological degradation	biological degradation
	test substance	reference substance	test substance	reference substance
d	mg/mg	mg/mg	%	%
1	0.013	0.04	1.1	1.4
2	0.013	0.077	1.1	2.7
5	0.07	0.372	5.4	13.1
12	0.176	0.689	13.8	24.2
17	0.592	0.803	46.3	28.3
23	0.726	0.984	56.8	34.6
28	0.763	1.016	59.7	35.8

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

The submission substance was tested for ready biodegradability according to OECD 301 D (closed bottle test). At day 28, 60% biodegradation (rounded from 59.7%) was reached, however the 14 day window (for the closed bottle test) was failed. Therefore, the submission substance is regarded to be inherently biodegradable.

Executive summary:

The submission substance was tested for ready biodegradability according to OECD 301 D (closed bottle test) and compliant to GLP (reliability category 1). At day 28, 60% biodegradation (rounded from 59.7%) was reached, however the 10- respectively 14 day window (for the closed bottle test) was failed. Therefore, the submission substance is readiliy biodegradable but failing the 10-day window. According to guidance on information requirements and chemical safety assessment, part R.7B (section R.7.9.4.1), the submission substance may therefore be regarded as inherently biodegradable.