Registration Dossier
Registration Dossier
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EC number: 289-969-0 | CAS number: 90046-02-9 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Juniperus oxycedrus, Cupressaceae.
Endpoint summary
Administrative data
Description of key information
The ready biodegradability of the test item has been determined by the Manometric Respirometry Test according to the OECD Guideline No. 301F with GLP compliance.
The test item was investigated for its ready biodegradability in a manometric respirometry test over a period of 60 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure with a test item loading rate of103.3 mg/L corresponding to an oxygen demand of about 251.9 mg/L(ThODNH4). As a reference item sodium benzoate (103.3 mg/L corresponding to an oxygen demand of about 172.1 mg/L(ThODNH4)) was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. Degradation rate of test item calculated by the oxygen consumption of the aerobic activated sludge microorganisms after 60 days of incubation.
All validity criteria were met. Degradation of the positive control was 76% after 14/28/60 days, thus confirming the suitability of the aerobic activated sludge inoculum used.No inhibition on the microorganisms are observed.
The test item contains no nitrogen; therefore the evaluation of biodegradation has to be based ThODNH4. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the 10-day window, describing the period between reaching at least 10% degradation and 60% degradation. This period should not exceed 10 days. The mean biodegradation of 10% of test item was reached at day 4 (ThODNH4). At the end of the 10 -day window at day 14, the degradation of test item was 38% (ThODNH4) and therefore the 10 day window criterion was not passed.However the 10d window criterion is not relevant regarding CLP regualtion for complex mixture.The mean biodegradation after 28 days was 47% (ThODNH4). The test was extended to 60 days, as no plateau was reached within 28 days. The mean biodegradation of the replicates was 59% at the end of the test at day 60. Some replicates did pass the 60% treshold.
Therefore, test item is considered to be not readily biodegradable.
Additional information
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