2-[N-(2-cyanoethyl)-4-[(2,6-dichloro-4-nitrophenyl)azo]anilino]ethyl acetate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

activated sludge

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

pH:

7.7-7.9

Nominal and measured concentrations:

0, 64, 160, 400 and 1000 mg/L (nominal)

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Key result

Duration:

3 h

Dose descriptor:

IC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Results with reference substance (positive control):

3,5-dichlorophenol (IC50 3h): 9.1 mg/L

Table 1. Results

Test substance (mg/L)	O2 -consumption (in cm)	Time (in cm)	mg O2 pro min./L	% of control	% inhibition
1000	5.3	5.1	0.83	96.19	3.81
400	5.5	5.2	0.85	97.90	2.10
160	5.0	4.8	0.83	96.41	3.59
64	5.3	4.9	0.87	100.11	0.00

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the nominal 3 h IC50 and IC10 of the test substance were >1000 mg/L (activated sludge).

Executive summary:

A study was conducted to determine the toxicity of the test substance to activated sludge bacteria according to OECD Guideline 209 (activated sludge respiration inhibition test). Activated sludge was exposed to nominal concentrations of 0, 64, 160, 400 and 1000 mg/L for 3 h. The positive reference 3,5 -dichlorophenol was run in parallel and presented an IC50 of 9.1 mg/L. Temperature, pH and oxygen inhibition were recorded at the end of the test. Under the study conditions, the nominal 3 h IC50 and IC10 of the test substance were >1000 mg/L (Schnalke, 1997).

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

other: sludge

Details on inoculum:

Source: microorganisms from activated sludge of waste-water treatment plants.
Dry sludge: 1.68 g/L

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test temperature:

22.1°C

pH:

7.0-7.8

Nominal and measured concentrations:

0, 1.0, 3.2, 10, 32, 100 and 320 mg/L (nominal)

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol (0, 3.2, 10 and 32 mg/L)

Key result

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks on result:

other: corresponding to > 97 mg a.i./L

Remarks:

purity of 30.3%

Results with reference substance (positive control):

3,5-dichlorophenol (3 h IC50): 9.5 mg/L

Table 1. Results

Test substance (mg/L)	O2 -consumption (in cm)	Time (in cm)	mg O2 pro min./L	% of control	% inhibition
320	4.0	3.6	0.89	96.74	3.26
100	4.2	3.8	0.88	95.65	4.35
32	4.2	3.7	0.91	98.91	1.09
10	3.9	3.6	0.87	94.57	5.43

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the nominal 3 h IC50 of the test substance was >320 mg/L, corresponding to 97 mg a.i./L (sludge).

Executive summary:

A study was conducted to determine the toxicity of the test substance to sludge microorganisms according to OECD Guideline 209 (respiration inhibition test). Activated sludge was exposed to nominal concentrations of 0, 1.0, 3.2, 10, 32, 100 and 320 mg/L for 3 h. The positive reference 3,5 -dichlorophenol was run in parallel and presented an IC50 of 9.5 mg/L. Temperature, pH and oxygen inhibition were recorded at the end of the test. Under the study conditions, the nominal 3 h IC50 of the test substance was >320 mg/L, corresponding to 97 mg a.i./L (Schnalke, 1992).

Reference 3

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Version / remarks:

ISO 8192-1986

Qualifier:

according to guideline

Guideline:

other: ETAD 103

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge

Details on inoculum:

Inoculum: 6.0 g/L

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Nominal and measured concentrations:

0, 100, 1000 and 10000 mg/L (nominal)

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

Respiration rates (mg/L*h): 18, 18 and 15 for 100, 1000 and 10000 mg/L test substance, respectively
Inhibition (%): 0, 0 and 17 for 100, 1000 and 10000 mg/L test substance, respectively

Results with reference substance (positive control):

3,5-dichlorophenol (20.0 mg/L, inhibition %): 67

Conclusions:

Under the study conditions, the nominal 3 h IC50 of the test substance was >10000 mg/L (activated sludge).

Executive summary:

A study was conducted to determine the toxicity of the test substance to sludge microorganisms according to OECD Guideline 209 (respiration inhibition test), ISO 8192 -1986 and ETAD 103. Activated sludge was exposed to nominal concentrations of 0, 100, 1000 and 10000 mg/L for 3 h. The positive reference 3,5 -dichlorophenol was run in parallel and presented an inhibition of 67%. Under the study conditions, the nominal 3 h IC50 of the test substance was >10000 mg/L (Kanne, 1989).

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

EC10 or NOEC for microorganisms:

1 000 mg/L

Additional information

A study was conducted to determine the toxicity of the test substance to activated sludge bacteria according to OECD Guideline 209 (activated sludge respiration inhibition test). Activated sludge was exposed to nominal concentrations of 0, 64, 160, 400 and 1000 mg/L for 3 h. The positive reference 3,5 -dichlorophenol was run in parallel and presented an IC50 of 9.1 mg/L. Temperature, pH and oxygen inhibition were recorded at the end of the test. Under the study conditions, the nominal 3 h IC50 and IC10 of the test substance were >1000 mg/L (Schnalke, 1997).

A study was conducted to determine the toxicity of the test substance to sludge microorganisms according to OECD Guideline 209 (activated sludge respiration inhibition test). Activated sludge was exposed to nominal concentrations of 0, 1.0, 3.2, 10, 32, 100 and 320 mg/L for 3 h. The positive reference 3,5 -dichlorophenol was run in parallel and presented an IC50 of 9.5 mg/L. Temperature, pH and oxygen inhibition were recorded at the end of the test. Under the study conditions, the nominal 3 h IC50 of the test substance was >320 mg/L, corresponding to 97 mg a.i./L (Schnalke, 1992).

A study was conducted to determine the toxicity of the test substance to sludge microorganisms according to OECD Guideline 209 (respiration inhibition test), ISO 8192 -1986 and ETAD 103. Activated sludge was exposed to nominal concentrations of 0, 100, 1000 and 10000 mg/L for 3 h. The positive reference 3,5 -dichlorophenol was run in parallel and presented an inhibition of 67%. Under the study conditions, the nominal 3 h IC50 of the test substance was >10000 mg/L (Kanne, 1989).