Reaction products of acetic acid with formaldehyde and a mixture of 1-, 2-, 3-,octene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May - July 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Tetrahydro-3-pentyl-2H-pyran-4-yl acetate

EC Number:

242-640-5

EC Name:

Tetrahydro-3-pentyl-2H-pyran-4-yl acetate

Cas Number:

18871-14-2

Molecular formula:

C12H22O3

IUPAC Name:

3-O-acetyl-1,5-anhydro-2,4-dideoxy-2-pentylpentitol

Constituent 2

Reference substance name:

3-butyltetrahydro-5-methyl-2H-pyran-4-yl acetate

EC Number:

253-863-2

EC Name:

3-butyltetrahydro-5-methyl-2H-pyran-4-yl acetate

Cas Number:

38285-49-3

Molecular formula:

C12H22O3

IUPAC Name:

3-O-acetyl-1,5-anhydro-2-butyl-2,4-dideoxy-4-methylpentitol

impurity 1

Reference substance name:

3-pentyltetrahydro-2H-pyran-4-ol

Cas Number:

24646-98-8

Molecular formula:

C10H20O2=

IUPAC Name:

3-pentyltetrahydro-2H-pyran-4-ol

impurity 2

Reference substance name:

Likely acetylated constituents, MW 214

Molecular formula:

n/a

IUPAC Name:

Likely acetylated constituents, MW 214

impurity 3

Reference substance name:

Likely acetylated constituents

Molecular formula:

n/a

IUPAC Name:

Likely acetylated constituents

impurity 4

Reference substance name:

Unknown constituent, MW 172

Molecular formula:

n/a

IUPAC Name:

Unknown constituent, MW 172

Test material form:

liquid

Details on test material:

UVCB substance

Specific details on test material used for the study:

Givaudan Product Code: 6347001
Batch Number: PE00138468

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The determination of the actual test item concentrations was based on the measurement of the sum of all components of the test item. For this, duplicate samples were taken from each test concentration and the control at the start and at the end of the two 24-hour test medium renewal periods.
The concentrations of JASMONYL LG were analyzed in one of the duplicate test medium samples from the nominal concentrations of 32, 56 and 100 mg/L from all sampling dates. The samples of the nominal test concentrations of 10 and 18 mg/L were not analyzed since these concentrations were below the 48 hour NOEC determined in this test and, thus, were not relevant for the interpretation of the biological results.

Test solutions

Vehicle:

no

Details on test solutions:

Reconstituted test water (ISO Test water) according to the OECD Guideline No. 202 was used in the study. It consisted of analytical grade salts dissolved in purified water.
Since the test item was determined to be volatile, the test was performed in glass vessels completely filled (without headspace) with 60 mL test medium and tightly sealed with glass stoppers to avoid losses of the volatile substance by evaporation (closed system).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The study was performed with young daphnids of the species Daphnia magna Straus. A clone of this species (originally from the Daphnia Collection of the University of Basel/Switzerland in 2015) is successfully bred in IES Ltd Laboratories.
At the start of the test, the organisms used in the test were 6 to 24 hours old and were not first brood progeny.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

The test was performed in a temperature-controlled room with continuous monitoring of the room temperature. The water temperature was in the range of 21-22 °C.

pH:

At the beginning and end of the test medium renewal periods, the pH values of the test media were between 7.6 and 7.9.

Dissolved oxygen:

The dissolved oxygen concentrations in the test media and control were at least 8.0 mg/L.

Nominal and measured concentrations:

Based on the results of the range-finding study the following nominal concentrations of JASMONYL LG were selected for the main test: 10, 18, 32, 56 and 100 mg/L applying a spacing factor of 1.8. Additionally, a control (test water without test item) was tested in parallel.

Details on test conditions:

A semi-static test with test medium renewal after 24 hours was performed in a closed system (without headspace) to keep the concentrations of the test item in the test media as constant as possible during the test period of 48 hours. After 24 hours, the test organisms were transferred by wide-bore pipette in to clean test vessels with freshly prepared test medium of the corresponding concentration.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

51 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence limits: 47-56

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: and 48-hour EC0

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The measured concentrations of the test item in the analytically analyzed test media of nominal 32 to 100 mg/L were between 87 and 102% of the nominal values at the start of the two test medium renewal periods. Thus, the correct dosage of the test item was confirmed. At the end of the test medium renewal periods, the chromatographic profile of the test item was not significantly changed and represented 84 to 98% of the nominal values (sum of peaks). The analytical results showed that the test design chosen (closed system and semi-static design) was appropriate for a sufficiently constant test item exposure of the daphnids. Therefore, the biological results are based on the nominal concentrations of the test item.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC50 of Jasmonyl LG was calculated to be 51 mg/L with 95% confidence limits of 47 and 56 mg/L.