Trisodium 5-[[4-chloro-6-(ethylphenylamino)-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[(4-methyl-2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11.5.2000 - 29.6.2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Test No. L 49-Acute Immobilisation test of Daphnia magna, Chemical formulation and waste waters

Deviations:

no

Principles of method if other than guideline:

Daphnia are exposed to various concentrations of the test substance in water during 48 hours. The acute toxicity is monitored as immobilisation of daphnia after 24 and 48 hours. The results are expressed in mg/L.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: own laboratory breeding
- Daphnia magna Straus were obtained by aperiodic parthenogenesis from own laboratory breeding
- Young daphnia born within 24 hours are segregated to the handling container from which they entered the experiment.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Details on test conditions:

Main test No. 1:
Oxygen content in the control: 8.5 – 8.6 mg.L-1
Oxygen content in the tested concentrations: 8.3 – 8.6 mg.L-1
pH of the control: 7.87 – 7.93
pH of the tested concentrations: 7.87 – 8.04
Temperature: 21.7 - 21.8 °C

Main test No. 2:
Oxygen content in the control: 8.1 – 8.5 mg.L-1
Oxygen content in the tested concentrations: 8.1 – 8.5 mg.L-1
pH of the control: 7.71 – 7.93
pH of the tested concentrations: 7.82 – 8.05
Temperature: 21.1 - 21.6 °C

Dilution water:
The dilution water was prepared just before the test by admixing 2,5 mL of stored solutions A, B, C and D into 1 000 mL of distilled water.
A. 117.6 g CaCl2.2H2O in 1 L of distilled water
B. 49.3 g MgSO4.7H2O in 1 L of distilled water
C. 25.9 g NaHCO3 in 1 L of distilled water
D. 2.3 g KCl in 1 L of distilled water

The stock solution for the preliminary test No.1: 1.0 g.L-1
The stock solution for the preliminary test No.2, 3 and for the main test No.1, 2: 10.0 g.L-1

The concentration tested in the Preliminary tests:
1. 1 000, 750, 500 and 250 mg.L-1
2. 10 000, 8 000, 6 000, 4 000 and 2 000 mg.L-1
3. 5 500, 5 000, 4 500, 4 000, 3 500, 2 500 mg.L-1

The concentration tested in the Main test No.1, 2:
5502, 5001, 4501, 4001, 3501, 3001, 2501, 2001 mg.L-1

The volume of the test solution was 50 mL in each test beaker.

Preliminary Test No. 1, 2, 3: 10 animals
Main test No.1, 2: 10 animals in two parallel rows (20 animals)

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

3 313 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2 723 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: not observed
- Observations on body length and weight: not observed
- Other biological observations: not observed
- Mortality of control: not observed

Results with reference substance (positive control):

-Results with reference substance valid: yes
The sensitivity of the test species and correctness of test performance is periodically verified in three-month period by testing with the reference substance, potassium dichromate.

Validity criteria fulfilled:

yes

Executive summary:

The test substance Reactive Red 45:1 was tested in acute immobilisation test on Daphnia magna.

Results:

24h-EC50=3313 mg/l

48h-EC50=2723 mg/l

The test quality criteria

These quality criteria are given in the guideline.

1) Immobilisation in the controls must not exceed 10 % at the end of the test.

2) The dissolved oxygen concentrations (throughout the test) should remain above 3 mg·L^-1.

Description of key information

The 48h-EC50 of Reactive Red 45:1 in Daphnia magna was determined to be 2723 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Remarks:

mobility

Effect concentration:

2 723 mg/L

Additional information

The test substance Reactive Red 45:1 was tested in acute immobilisation test on Daphnia magna.

Results:

24h-EC50=3313 mg/l

48h-EC50=2723 mg/l