Phosphoric acid, mono- and di-isotridecyl esters, potassium salts

Short-term toxicity to fish

Description of key information

Based on the results of the read across studies, the 96 h-LL50 for the test substance to Cyrpinus Carpio was determined to be above 100% of a saturated solution prepared at a loading rate of 100 mg/L..

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Study 1. A study was conducted to determine the short-term toxicity to fish of the read across substance, mono- C12 PSE, K+, according OECD Guideline 203 and EU Method C.1, in compliance with GLP. Following a preliminary range-finding test, rainbow trouts were exposed, in two groups of seven, to an aqueous dispersion of the test material, at a single concentration of 100 mg a.i./L for a period of 96 h under semi-static test conditions. A negative control group with fish not exposed to the test substance was included in the experiment. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 h after start of exposure and then daily throughout the test until termination after 96 h. No mortality was observed during the experiment. Based on the results of the read across study, the 96 h EC50 and NOEC (rainbow trout) were determined to be >100 and 100 mg a.i./L, respectively (Wetton, 2002).

Study 2. A study was conducted to determine the acute toxicity of the read across substance, mono- and di- C12 -18 PSE, Na+, to fish according to OECD Guideline 203 and EU Method C.1, in compliance with GLP. Seven fish (Cyrpinus carpio) per group were exposed to a control (medium alone) and to 100% of the saturated solution (SS). The total exposure period was 96 h and the experiment was realised under static conditions. Due to the complex nature of the test substance, specific analyses proved to be difficult to obtain and it was decided to analyse the Total Organic Carbon (TOC) concentration as a surrogate measure. Samples for TOC concentrations were taken at the start and after 24 and 96 h of exposure. The TOC analysis performed on samples taken from 100% of the SS should be regarded as indicative because of the low carbon concentrations. Effect parameters were based on the percentages of the saturated solution prepared at a loading rate of 100 mg/L. No mortality or clinical effects were observed at the limit concentration and in the control during the test period. Based on the results of the read across study, the 96 h-LL50 of the test substance was above 100% of a saturated solution prepared at a loading rate of 100 mg/L (Bouwman, 2017).