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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993-07-01 to 1993-07-15
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD and GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
no
Principles of method if other than guideline:
Limit Test
GLP compliance:
yes
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
(3H)-benzofuran-2-one
EC Number:
209-052-0
EC Name:
(3H)-benzofuran-2-one
Cas Number:
553-86-6
Molecular formula:
C8H6O2
IUPAC Name:
2,3-dihydro-1-benzofuran-2-one
Details on test material:
- Name of test material (as cited in study report): 2-COUMARANONE
- Physical state: solid
- Lot/batch No.: D 2008
- Storage condition of test material: in a plastic flask, at room temperature

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
corn oil
Doses:
2000 mg/kg using a dose volume of 10 ml/kg
No. of animals per sex per dose:
5
Control animals:
no

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Remarks on result:
other: limit test
Mortality:
male: 0/5
female: 0/5
Clinical signs:
other: A slight decrease in the spontaneous activity was observed in all the animals during the first hours. From day 2, the general behaviour was not affected by treatment with the test substance.
Gross pathology:
A macroscopic examination revealed no abnormalities in the animals sacrificed at the end of the study.
Other findings:
No other findings.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
Under the experimental conditions, the LD50 of the test substance 2-COUMARANONE when administered by oral route in rats was higher than 2000 mg/kg.
Executive summary:

The test substance was administered by oral route to a group of 10 fasted Sprague-Dawley rats (5 males and 5 females). The test substance was suspended in corn oil and then administered by gavage at a dose of 2000 mg/kg and using a dose volume of 10 ml/kg. The animals were checked for clinical signs, mortality and body weight gain for a period of 14 days following the single administration of the test substance. A necropsy was performed on each animal sacrificed at the end of the study.

A slight decrease in the spontaneous activity was observed in all the animals during the first hours. From day 2, the general behaviour was not affected by treatment with the test substance.

The body weight gain of the animals was normal.

No deaths occurred at 2000 mg/kg.

A macroscopic examination revealed no abnormalities in the animals sacrificed at the end of the study.

Under the experimental conditions, the LD50 of the test substance 2-COUMARANONE when administered by oral route in rats was higher than 2000 mg/kg.