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EC number: 701-177-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Auto flammability
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- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
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- Stability: thermal, sunlight, metals
- pH
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- Additional physico-chemical information
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- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Nanomaterial aspect ratio / shape
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
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- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 May - 14 Jun 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge was collected on 13 May 2005 from the municipal waste water treatment plant Neustadt/Weinstrasse, Lachen-Speyerdorf
- Method of cultivation: The sludge was continuously aerated and washed with tap water and OECD medium
- Concentration of suspended solids: 12.78 g/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium as recommended by the guideline
- Test temperature: 21 ± 1 °C
- Aeration of dilution water: All vessels were aerated overnight with CO2-free air prior to test start and during the test
- Suspended solids concentration: 25 mg/L
TEST SYSTEM
- Culturing apparatus: 2 L Glass bottles, 1500 mL fill volume
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Aeration with CO2-free air
- Details of trap for CO2: Outgoing air from the test vessels passed two serial bottles containing 0.25 M NaOH solution
- Other:
SAMPLING
- Sampling frequency: Measurement of evolved CO2 on days 2, 4, 6, 8, 10, 14, 21, 28 and 29
- Sampling method: 0.5 mL aliquots of the NaOH solution was sampled at each time point and analysed using a TOC 5050A (Shimadzu)
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 vessels
- Abiotic sterile control: 1 vessel
- Toxicity control: 1 vessel - Reference substance:
- aniline
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 85.2
- Sampling time:
- 28 d
- Details on results:
- - The toxicity control attained 98.3% degradation.
- Abiotic degradation was 26.6%. - Results with reference substance:
- The reference substance attained 79.9% degradation after 28 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
Reference
Table 1: Percentage of Degradation
Day |
% Degradation |
|||
Reference |
Test Item (mean of duplicates) |
Abiotic Control |
Toxicity Control |
|
2 |
2.6 |
1.7 |
3.4 |
3.2 |
4 |
26.5 |
24.1 |
3.1 |
53.5 |
6 |
56.1 |
47.5 |
11.3 |
68.7 |
8 |
66.5 |
57.4 |
16.0 |
75.8 |
10 |
71.1 |
61.3 |
8.6 |
79.7 |
14 |
76.2 |
72.9 |
17.6 |
86.7 |
21 |
85.0 |
88.3 |
22.9 |
97.5 |
28 |
77.6 |
83.0 |
28.0 |
99.6 |
29 |
79.9 |
85.2 |
26.6 |
98.3 |
Description of key information
Readily biodegradable: 85.2% in 28 d (OECD 301 B)
Key value for chemical safety assessment
Additional information
There is one study available investigating the ready biodegradability of N-methyl-N-(C18-(unsaturated)alkanoyl)glycine (EC 701-177-3). This study was conducted according to the OECD guideline 301B using non-adapted activated sludge from a municipal waste water treatment plant. 20 mg/L test substance (based on TOC) was inoculated with 25 mg/L domestic, non-adapted activated sludge for 28 d under aeration with CO2-free air. Inoculum blanks, an abiotic sterile control and a toxicity control were run in parallel. CO2 was trapped in bottles containing 0.25 M NaOH solution and the evolution of CO2 concentration was followed by TOC analysis in regular intervals. After 28 d incubation, the test item was degraded by 85.2% in terms of CO2 evolution. Thus, it can be concluded that N-methyl-N-(C18-(unsaturated)alkanoyl)glycine (EC 701-177-3) is readily biodegradable according to the OECD guideline criteria.
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