Ethyl ethyl(phenyl)carbamate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March 6, 2017 - March 14, 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Key result

Water solubility:

ca. 1.424 g/L

Conc. based on:

test mat.

Incubation duration:

>= 1 - <= 3 d

Temp.:

20.1 °C

pH:

>= 6.6 - <= 6.65

Determination of solubility in water at one pH value

In the test, the following flasks were used:

Flasks 1 (samples 1 & 2) – for one day at 30ºC;

Flasks 2 (samples 3 & 4) – for two days at 30ºC;

Flask 3 (samples 5 & 6) – for three days at 30ºC.

The individual results from each of three flasks, which should not differ by more than 15%, are averaged.If the concentrations measured in at least two last samples do not differ by more than 15% the test is satisfactory.

Water solubility is expressed in mass of solute per volume of solution.

Table No.1: Calibration solution

Day	Weight [mg]	Dilution [mL]	Cw [mg/mL]	Peak area		Average peak area Aw	f	Average  f
1	27.05	25-1-25-2-10	0.008137	459244	461808	460526	0.000018	0.0000177
	27.05	25-1-25-3-10	0.012205	691885	690312	691098.5	0.000018
2	27.05	25-1-25-2-10	0.008137	460412	459798	460105	0.000018	0.0000177
	27.05	25-1-25-3-10	0.012205	692193	690565	691379	0.000018
3	27.05	25-1-25-2-10	0.008137	468612	467501	468056.5	468612	0.0000173
	27.05	25-1-25-3-10	0.012205	707369	706459	706914	707369

Table No.2 : Study solution in water

Sample day/ flask		Weight of the sample [mg]	Dilution [mL]	Cp [mg/mL]	Peak area		Average peak area Ap	X [g/kg]
1	1	991.85	10-2-25	7.935	639750	636922	638336	1.421
	2	995.70		7.966	630102	633965	632033.5	1.402
2	3	1014.55	10-2-25	8.116	653252	652463	652857.5	1.421
	4	1010.05		8.080	653106	654046	653576	1.429
3	5	1008.95	10-2-25	8.072	652783	651717	652250	1.427
	6	1003.00		8.024	655766	652294	654030	1.440

Table No.3 : Results of the preliminary test

0.1g in x mL of solution	0.1	0.5	1	2	10	50
Soluble (+/-)	-	-	-	-	-	+
Approximate solubility, g/L	> 1000	1000 to 200	200 to 100	100 to 50	50 to 10	10 to 2

x – water of pH 5.7 

Table No.4: Result of Test item solubility in water:

Mixing time at 30 ºC [days, flasks]	1		2		3
Samples	1	2	3	4		5	6
Temperature, ºC	20.1		20.1		20.0
pH	6.60		6.61		6.65
Concentration of test item, g/kg	1.421	1.402	1.421	1.429		1.427	1.440
Average concentration of test item,g/kg	1.412		1.425		1.434
Average concentration of test item, g/kg	1.424

Conclusions:

The solubility of test item in water was determinated to be 1.424 g/L.

Executive summary:

In accordance with OECD TG 105 and GLP study, determination of solubility in water at one pH value was determined by flask method. The preliminary test was performed to estimate the quantity of test item and about five times the above quantity of determinated in the preliminary test was weighted into three glass vessels of 100 mL and distilled water was added too.Three closed vessels were incubated at 30ºC with magnetic stirrers. After one day, one of the flask was equilibrated for 24 h at the test temperature (20 ± 0.5)ºC with occasional shaking. The other two flasks were treated similarly after initial equilibration at 30ºC for two and three days, respectively. The analysis was determined by a HPLC method. In conclusion, the solubility of test item in water was determinated to be 1.424 g/L.

Description of key information

Key study: OECD guideline 105 and GLP study. The solubility of test item in water is 1.424 g/L

Key value for chemical safety assessment

Water solubility:

1.424 g/L

at the temperature of:

20.1 °C

Additional information

Key study: OECD TG 105 and GLP study. Determination of solubility in water at one pH value was determined by flask method. The preliminary test was performed to estimate the quantity of test item and about five times the above quantity of determinated in the preliminary test was weighted into three glass vessels of 100 mL and distilled water was added too.Three closed vessels were incubated at 30ºC with magnetic stirrers from 1 to 3 days. The analysis was determined by a HPLC method.Test was satisfactory because two last test did not differ by more than 15% and in conclusion, the solubility of test item in water was determinated to be 1.424 g/L.