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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May 1995
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc
EC Number:
283-381-8
EC Name:
Bis[bis(3,5,5-trimethylhexyl)dithiocarbamate-S,S']zinc
Cas Number:
84604-96-6
Molecular formula:
C38H76N2S4Zn
IUPAC Name:
bis({[bis(3,5,5-trimethylhexyl)carbamothioyl]sulfanyl})zinc
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Selenastrum sp.
Details on test organisms:
Selenastrum capricornutum to test material ( Printz) strain CCAP 278/4. Liquid cultures were obtained from Institute of freshwater ecology, Ferry house, Ambleside, Cumbria

Study design

Test type:
static
Water media type:
other: culture media pH 8
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Test temperature:
24°C
pH:
mean 9.7
Nominal and measured concentrations:
nominal 0.32mg/l

at 0 hours, measured test oncentration was 0.34mg/l and 0.339 mg/l but after 96 hours ( despite the stability data performed prior to study) the concentration of test item had decreased to 78 & 68% of nominal concentration- there may have been adsorption of test item to glassware.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
>= 0.32 mg/L
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
>= 0.32 mg/L
Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
>= 0.32 mg/L

Any other information on results incl. tables

stability of test material over 96 hours was proved and can be seen in attached summary

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
There was no recorded adverse effect observed on growth rate or growth biomass due to test item in 96 hour algae acute study. NOEC > 0.32mg/l ( at limit of solubility)