Resinoid of Cistus ladaniferus (Cistaceae) obtained from labdanum gum by ethanol extraction

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 07 to 14, 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The substance is considered to be adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Version / remarks:

adopted 02. Oct. 2012

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Version / remarks:

Council Regulation (EC) No 440/2008 of 30 May 2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

inspected on 29 and 30 November 2010 / signed on 11 April 2011

Type of method:

pycnometer method

Key result

Type:

density

Density:

1.067 g/cm³

Temp.:

20 °C

Key result

Type:

relative density

Density:

1.067 other: unitless

Temp.:

20 °C

Table 4.4/1: Measured Values

 

First Determination				Error rel.	Error abs.
Temperature	20.2	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liq.	Pycn. + test item	Pycn. + test item + repl. liq.
Mass in g	36.9423	61.7968	61.8041	40.8296	62.0541
Temperature in °C	20.0	20.2	20.0	20.0	20.0
Volume of Pycnometer	24.8992	cm^3
Density of Replacement Liquid	0.9985			Error rel.	Error abs.
Density of Test Item	1.0671	g/cm^3		0.0533%	0.0006
Second Determination				Error rel.	Error abs.
Temperature	20.0	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liq.	Pycn. + test item	Pycn. + test item + repl. liq.
Mass in g	36.9419	61.8018	61.7977	40.9265	62.0536
Temperature in °C	19.8	20.0	20.2	19.8	20.0
Volume of Pycnometer	24.9047	cm^3
Density of Replacement Liquid	0.9980			Error rel.	Error abs.
Density of Test Item	1.0665	g/cm^3		0.0521%	0.0006

Results of the single determinations with the respective error as well as the mean value and its standard deviation are presented in the following tables:

Table 4.4/2: Results of Single Determinations

 

Determination	Density in g/cm^3	Error of Determ. in g/cm^3
1	1.0671	0.0006
2	1.0665	0.0006

 

Table 4.4/3 Results – Mean Values

 

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
Volume of Pycnometer	24.90	cm^3	0.0038	0.02%
Density of Test Item	1.0668	g/ cm^3	0.0004	0.04%
Density of Test Item	1066.8	Kg/m^3	0.4	0.04%
Relative Density of Test Item	1.0668		0.0004	0.04%

 

The density of the test item was calculated as 1.0668 ± 0.0004 g/cm³ at 20.0 ± 0.2 °C, which is the mean of two independent determinations ± the standard deviation. Standard deviation shows good correlation of the two determinations. Therefore, the study is considered valid.

Conclusions:

The density of the test substance was calculated as 1.0668 ± 0.0004 g/cm3 at 20.0 ± 0.2 °C and the relative density is determined as D20/4 = 1.0668 ± 0.0004
Denser than water

Executive summary:

The density of the test substance was measured under GLP according OECD 109 / EU A3 guideline, using the pycnometer method.

Two determinations were performed and the mean value was retained.

This result was then converted to relative density towards water at 4 °C.

Mean density: 1.0668 ± 0.0004 g/cm³at 20.0 ± 0.2 °C

Mean relative density (D20/4) : 1.0668

Description of key information

The density of the test item was calculated as 1.0668 ± 0.0004 g/cm3 at 20.0 ± 0.2 °C and the relative density is determiend as D20/4 = 1.0668

Key value for chemical safety assessment

Relative density at 20C:

1.067

Additional information

A fully reliable experimental study, conducted under GLP according to OECD 109 /EU A3 guideline, using pycnometer method, is available. It is considered as a key study, and the result is retained as key data.