N-(2-chloroethyl)-N-ethylaniline

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-03-19 - 1992-04-16

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test)

Version / remarks:

July 1990

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-(2-chloroethyl)-N-ethylaniline

EC Number:

245-082-0

EC Name:

N-(2-chloroethyl)-N-ethylaniline

Cas Number:

22564-43-8

Molecular formula:

C11H16ClN

IUPAC Name:

N-(2-chloroethyl)-N-ethylaniline

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): laboratory activated sludge plant run with municipal wastewater (Wupperverband)
- Pretreatment: none
- Concentration of sludge: 0.5 mL/900 mL

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

20 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: mineral medium
- Test temperature: 20-24 °C
- Continuous darkness: yes

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Key result

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

100% degradation has been determined with the reference substance aniline.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The study was conducted under GLP according to EU method C.4-B on the registered substance itself. The method is to be considered scientifically reasonable and suitable for the test item with some deficiencies in documentation, but the available information allows the conclusion that the test was properly conducted. Hence, the results can be considered as reliable to assess ready biodegradability of the test substance.
Biodegradation = 0% after 28 d; N-(2-chloroethyl)-N-ethyl-3-methylaniline needs to be classified as not readily biodegradable.

Executive summary:

N-(2-chloroethyl)-N-ethyl-3-methylaniline was assessed with regard to its biodegradability in an EU method C.4 -B guideline study under GLP at a concentration of 20 mg/L DOC for 28 days. Aniline served as positive control. Biodegradation was 0%, the test substance is not readily biodegradable. The study is classified as acceptable and meets the respective guideline requirements.