Reaction product of resin acid and rosin acid, 12-Hydroxyoctadecanoic and stearic acid with dipentaerythritol

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 23, 2007 - March 31, 2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 7-2-A
- Expiration date of the lot/batch: April 30, 2010
- Purity test date: not stated

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature at about 20°C
- Stability under test conditions: not stated
- Solubility and stability of the test substance in the solvent/vehicle: not stated
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not stated

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: none
- Preliminary purification step (if any): none
- Final dilution of a dissolved solid, stock liquid or gel: 376.3 µg/ml

Radiolabelling:

no

Test temperature:

20 °C

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: Merck-Hitachi autosampler L-7200
Merck-Hitachi pump L-6200
Merck-Hitachi detector L-4000 (channel A)
Sedex85, ELSD detector (channel B)
- Type: HPLC
- Type, material and dimension of analytical (guard) column: LiChrospher 100 CN, particle size 5µm, 250 mm x 4 mm
- Detection system: UV/Vis 210 nm for reference items and sodium nitrate
ELSD for Test Item, pyrazophons and diclofop-methyl reference items

MOBILE PHASES
- Type: A: Water/methanol (45:55; v/v)
B: Tetrahydrofurane
- Experiments with additives carried out on separate columns: no
- pH: 6.6
- Solutes for dissolving test and reference substances: tetrahydrofurane for test item
methanol/Mobile Phase A for reference substances

DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column (Sodium nitrate)

REFERENCE SUBSTANCES
- Identity: acetanilide, ioproturon, linuron, pyrazophos, diclofop-methyl, 2,4-DDT

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 20 µL of 376.3 µg/ml solution
- Quantity of reference substances: ca. 100 µg/ml
- Intervals of calibration: 6

REPETITIONS
- Number of determinations: twice

EVALUATION
- Calculation of capacity factors k': (tR-t0)/t0
- Calculation of retention times:
The mean value of the difference of retention times between UV signal and ELSD signal of pyrazophos and dicolfop-methyl was applied to the ELSD retention times of Salacos 168 ARV
- Determination of the log Koc value: plotting of log k' versus log KOC, using linear regression, determination of log KOC of test item by linear regression formula

Key result

Type:

Koc

Value:

> 426 580 dimensionless

pH:

6.6

Temp.:

20 °C

Matrix:

CN column

Key result

Type:

log Koc

Value:

> 5.63 dimensionless

pH:

6.6

Temp.:

20 °C

Matrix:

CN column

Details on results (HPLC method):

- Retention times of reference substances used for calibration:
Sodium nitrate (UV) 1.35 min
Acetanilide (UV) 3.24 min
Isoproturon (UV) 3.95 min
Linuron (UV) 4.97 min
Pyrazophos (UV) 6.70 min
Diclfop-methyl (UV) 7.97 min
2,4-DDT (UV) 12.90 min

Pyrazophos (ELSD) 6.93 min
Diclfop-methyl (ELSD) 8.158 min

- Details of fitted regression line (log k' vs. log Koc):
slope = 0.176
y-axiy intercept = -0.050
coefficient of regression (r) = 0.999

- Average retention data for test substance:
29.251 min (corrected for UV)

The retention time of the test item is higher than the retention of the highest reference item used (2,4 -DDT) with log KOC 5.63, which is equal to a KOC value of 426580.

Therefore this value is taken as minimum value for Salacos 168 ARV.

Based on this, the mobility class of Salacos 168 ARV is immobile (> 5000).

Validity criteria fulfilled:

yes

Conclusions:

The log of the adsorption coefficient of Salacos 168 ARV was estimated to be log KOC > 5.63 which is equal to a KOC value of > 426580.

Executive summary:

The log of the adsorption coefficient (KOC) of Salacos 168 ARV was determined to be higher than that one of the highest reference item 2,4 -DDT using an HPLC method.

As result, the log KOC of Salacos 168 ARV can be stated to be: log KOC > 5.63 which is equal to a KOC value of > 426580.

This value indicates that Salacos 168 ARV will be adsorbed by organic carbon in soil. Salacos 168 ARV can be classified to be immobile.

Description of key information

One study performed using a HPLC method.

For chemical safety assessment, the highest values of the tested reference substances is given,

as the log k' value of Salacos 168 ARV is outside of the regression scope.

Key value for chemical safety assessment

Koc at 20 °C:

426 580

Additional information

[LogKoc: 5.63]