Ammonium (di C8-C10, branched, C9 rich, alkylnaphthalene sulphonate)

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study followed guidelines appropriate at the time of the study. 10 animals were tested at the very high concentration of 200 mg/L and no toxicity was evident.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Acute Inhalation Study. US FHSLA, CFR, Title.21, para. 191.10.

Deviations:

not specified

GLP compliance:

no

Test type:

fixed concentration procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid

EC Number:

939-714-0

Cas Number:

1474044-77-3

Molecular formula:

C18H30SO3 to C40H67SO3

IUPAC Name:

di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid

Test material form:

other: used as 50% solution in light naphthenic petroleum oil

Details on test material:

Synex 1052 (black-brown I iquid):
50% DNNSA (DI C8-C10, BRANCHED, C9 RICH, ALKYLNAPHTHALENE SULFONIC ACID)
50% Light Naphthalenic Petroleum Oil

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

The spray was introduced into the chamber via a nebulizer, was circulated and was directed away from the animals. The chamber was saturated with 200mg/liter.

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

Five male and five female Wistar rats, 200 - 300g. A group of five male and five female Wistar rats were exposed to an aerosol
of the test material in an inhalation chamber for one hour. The spray was introduced into the chamber via a nebulizer, was circulated and was directed away from the animals. The chamber was saturated with 200mg/liter, nominal, of test material prior to the period of exposure.
The rats were observed for 14 days following exposure.
exposu reo

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

ca. 1 h

Concentrations:

200 mg/L

No. of animals per sex per dose:

5 males, 5 females

Control animals:

no

Statistics:

None Required

Results and discussion

Preliminary study:

The study was a limit test without a preliminary exposure.

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 200 mg/L air

Based on:

test mat.

Exp. duration:

1 h

Effect levels 2

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 100 mg/L air

Based on:

act. ingr.

Exp. duration:

1 h

Mortality:

No mortality

Clinical signs:

other: No clinical symptoms or other adverse effects

Body weight:

See table - attached

Gross pathology:

No remarkable findings.

Any other information on results incl. tables

Body Weights of Wistar Rats

Rat No.	Sex	Body Weight - grams	Mortality
1	M	230	No
2	M	241	No
3	M	256	No
4	M	267	No
5	M	280	No
6	F	214	No
7	F	217	No
8	F	225	No
9	F	232	No
10	F	230	No

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Remarks:

Criteria used for interpretation of results: other: EPA Category IV ( practically non-toxic based on LC50 greater than 20 mg/L)

Conclusions:

A one-hour exposure to a 50% formulation of DNNSA at 200 mg/L was not toxic to male and female Wistar rats.

Executive summary:

Synex 1052, a 50% liquid formulation of DNNSA (DI C8-C10, BRANCHED, C9 RICH, ALKYLNAPHTHALENE SULFONIC ACID), was evaluated for acute inhalation toxicity. Five male and five female Wistar rats, (Body Wt. 200 - 300g) received a whole body exposure for one hour to an aerosol of the test material in an inhalation chamber. The spray was introduced into the chamber via a nebulizer, was circulated and was directed away from the animals. The chamber was saturated with 200 mg/liter, nominal, of test material prior to the period of exposure. The rats were observed for 14 days following exposure. In the one-hour exposure, and during the 14 day observation period, the test material did not cause mortality or clinical symptoms of poisoning. The 1-hour LC50 is > 200 mg/L of the 50% formulation of DNNSA. Based on the concentration of DNNSA, the 1-hour LC50 is > than 100 mg/L. The study reports that the result support classification as EPA Category IV (practically non-toxic based on LC50 greater than 20 mg/L).