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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2018
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Ruscus aculeatus, ext.
EC Number:
281-682-9
EC Name:
Ruscus aculeatus, ext.
Cas Number:
84012-38-4
Molecular formula:
not applicable
IUPAC Name:
Ruscus aculeatus, ext.
Test material form:
solid

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
other: OECD medium according to OECD 201
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
22.1 and 23.1°C
pH:
7.81 – 9.26
Nominal and measured concentrations:
6.25, 12.5, 25.0, 50.0 and 100.0 mg/L

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
33.37 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
64.39 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
10.23 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Yield
Key result
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
17.46 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Yield
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
48.52 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Yield
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Yield
Key result
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Yield

Any other information on results incl. tables

No abnormal shape of algal cells was noticed in the control and in the test concentrations of 6.25 and 12.5 mg/L (nominal) during the test. Thin and swollen cells were noticed in the concentration of 25.0 mg/L (nominal) together with the normal cells after 72 hours. Thin cells were noticed in the concentration of 50.0 mg/L (nominal) together with the normal cells after 48 hours and thin, swollen and normal cells were observed in the concentration of 50.0 mg/L (nominal) after 72 hours.

Thin and swollen cells were noticed in the concentration of 100.0 mg/L (nominal) together with normal cells after 48 hours and swollen cells were noticed in the concentration of 100 mg/L (nominal) together with normal cells after 72 hours.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
All validity criteria were met and therefore the study can be considered as valid.