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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
experimental data of read across substances
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two biodegradation studies as-
1., 2., 3., 4., 5., 6., 7. and 8.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material : Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4-bis[(2-ethylhexyl)amino]anthraquinone and 1-(butylamino)-4-(methylamino)anthraquinone and 1-(butylamino)-4-[(2-ethylhexyl)amino]anthraquinone and 1-[(2-ethylhexyl)amino]-4-(methylamino)anthraquinone
- Substance type : Organic
- Physical state : Solid
Oxygen conditions:
other: 1. to 6. aerobic (Biowin 1-6) and anaerobic (Biowin 7), 7. aerobic, 8. Not specified
Inoculum or test system:
other: 1. to 6. mixed populations of environmental microorganisms, 7. mixture of domestic waste water, surface soil and soil samples, 8. activated sludge (adaptation not specified)
Details on inoculum:
1. to 6. - No data available

7.
Mixed Inoculum Preparation (In-House): Domestic waste water, surface soil and soil samples from polluted sites receiving predominantly domestic waste was collected and were mixed to get diluted suspension. The inoculum was kept aerobic until being used for experiment by supplying organic and inorganic sources required by micro flora to sustain at controlled laboratory conditions. This gave the bacterial count as 107to 108 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism.

8.
- Concentration of sludge: 30 mg/l
Based on:
not specified
Remarks:
1. to 6.
Initial conc.:
32 mg/L
Based on:
test mat.
Remarks:
7.
Initial conc.:
100 mg/L
Based on:
test mat.
Remarks:
8.
Parameter followed for biodegradation estimation:
other: 1. to 6. Half-life
Parameter followed for biodegradation estimation:
other: 7. BOD and ThOD
Parameter followed for biodegradation estimation:
other: 8. BOD and HPLC
Details on study design:
1. to 6.
Using the computer tool BIOWIN v4.10 by US-EPA (EPIWIN) the aerobic as well as the anaerobic biodegradability of the test material can be estimated. The follwoing seven different models are used by the tool: Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI LInear Model, MITI Non-Linear Model and Anaerobic Model (calles Biowin 1-7, respectively). Due to this results the overall prediction of readily biodegradability is done for the desired chemical.

Biowin 1 and 2, are intended to convey a general indication of biodegradability under aerobic conditions, and not for any particular medium.
Biowin 1 (Linear model)
The fast biodegradation probability for any compound is calculated by summing, for all the fragments present in that compound, the fragment coefficient multiplied by the number of instances of the fragment in the compound (for MW, the value of that parameter is multiplied by its coefficient), and then adding this summation to the equation constant which is 0.7475. The summed values for each fragment coefficient multiplied by the number of instances appear in the "VALUE" column of the linear results screen.

Biowin 2 (Non-linear model)
Calculation of the fast biodegradation probability for any compound begins by summing, for all the fragments present in that compound, the fragment coefficient multiplied by the number of instances of the fragment in the compound (for MW, the value of that parameter is multiplied by its coefficient), then adding this summation to the equation constant which is 3.0087. The summed values for each fragment coefficient multiplied by the number of instances appear in the "VALUE" column of the non-linear results screen. The non-linear fast biodegradation probability is then calculated from the logistic equation as follows, where total = 3.0087 + the summation as described above:

Biowin 3 and 4 yield estimates for the time required to achieve complete ultimate and primary biodegradation in a typical or "evaluative" aquatic environment.

Biowin 5 and 6 are predictive models for assessing a compound’s biodegradability in the Japanese MITI (Ministry of International Trade and Industry) ready biodegradation test; i.e. OECD 301C. These models use an approach similar to that used to develop Biowin1 and 2. This protocol for determining ready biodegradability is among six officially approved as ready biodegradability test guidelines of the OECD (Organization for Economic Cooperation and Development). A total dataset of 884 chemicals was compiled to derive the fragment probability values that are applied in this MITI Biodegradability method. The dataset consists of 385 chemical that were critically evaluated as "readily degradable" and 499 chemicals that were critically evaluated as "not readily biodegradable".

Biowin 7, the anaerobic biodegradation model, is the most recent. As for the other Biowin models, multiple (linear) regression against molecular fragments was used to develop the model, which predicts probability of rapid degradation in the "serum bottle" anaerobic biodegradation screening test. This endpoint is assumed to be predictive of degradation in a typical anaerobic digester. Biowin7 estimates the probability of fast biodegradation under methanogenic anaerobic conditions; specifically, under the conditions of the "serum bottle" anaerobic biodegradation screening test (Meylan et al. 2007). A total of 169 compounds with serum bottle test data were identified for use in model development.

Out of seven different Biowin models, Biowin model 3 and 4 will help in estimating biodgeradability of the test chemical which was described as below-

Ultimate Biodegradation Timeframe and Primary Biodegradation Timeframe (Biowin 3 and 4)
These two models estimate the time required for "complete" ultimate and primary biodegradation.  Primary biodegradation is the transformation of a parent compound to an initial metabolite.  Ultimate biodegradation is the transformation of a parent compound to carbon dioxide and water, mineral oxides of any other elements present in the test compound, and new cell material. Then the rating was given to each model, which indicates the time required to achieve ultimate and primary biodegradation in a typical or "evaluative" aquatic environment. The ratings for each compound were averaged to obtain a single value for modeling.  The ultimate or primary rating of a compound is calculated by summing, for all the fragments present in that compound.

7.
TEST CONDITIONS
- Composition of medium: OECD mineral medium was used for the study
- Test temperature: 20± 1°C
- Continuous darkness: Yes
- Other: The water used in this study is deionized water.

TEST SYSTEM
- Culturing apparatus: The apparatus used in this study is Respirometer BOD Sensor System (Code: F102B0133; non-automatic pressure measurement sensor system) and Respirometric incubator (FOC 120i)) by VELP Scientifica.

CONTROL AND BLANK SYSTEM
- Inoculum blank: it contains only test inoculum
- Procedure control: contains reference compound and inoculum

8.
Standard type
Reference substance:
not specified
Remarks:
1. to 6., 8.
Reference substance:
other: 7. Sodium Benzoate
Parameter:
other: Half-life
Value:
50
Sampling time:
2.458 mo
Remarks on result:
other: not readily biodegradable as estimated by BIOWIN model
Parameter:
other: Half-life
Value:
50
Sampling time:
2.583 mo
Remarks on result:
other: not readily biodegradable as estimated by BIOWIN model
Parameter:
other: Half-life
Value:
50
Sampling time:
2.373 mo
Remarks on result:
other: not readily biodegradable as estimated by BIOWIN model
Parameter:
other: Half-life
Value:
50
Sampling time:
2.296 mo
Remarks on result:
other: not readily biodegradable as estimated by BIOWIN model
Parameter:
other: Half-life
Value:
50
Sampling time:
2.707 mo
Remarks on result:
other: not readily biodegradable as estimated by BIOWIN model
Parameter:
other: Half-life
Value:
50
Sampling time:
2.501 mo
Remarks on result:
other: not readily biodegradable as estimated by BIOWIN model
Parameter:
other: BOD and ThOD
Value:
10.212
Sampling time:
28 d
Remarks on result:
other: Other details not known
Parameter:
other: BOD
Value:
1
Sampling time:
28 d
Remarks on result:
other: Other details not known
Parameter:
other: HPLC
Value:
0
Sampling time:
28 d
Remarks on result:
other: Other details not known
Details on results:
1.
Biowin1 (Linear Model Prediction) : 0.2903: Does Not Biodegrade Fast
Biowin2 (Non-Linear Model Prediction): 0.0100: Does Not Biodegrade Fast
Biowin3 (Ultimate Biodegradation Timeframe): 2.4585: Weeks-Months
Biowin4 (Primary Biodegradation Timeframe): 3.4571: Days-Weeks
Biowin5 (MITI Linear Model Prediction) : -0.3005: Does Not Biodegrade Fast
Biowin6 (MITI Non-Linear Model Prediction): 0.0012: Does Not Biodegrade Fast
Biowin7 (Anaerobic Model Prediction): -2.1015: Does Not Biodegrade Fast
Ready Biodegradability Prediction: NO

2.
Biowin1 (Linear Model Prediction) : 0.3170: Does Not Biodegrade Fast
Biowin2 (Non-Linear Model Prediction): 0.0219: Does Not Biodegrade Fast
Biowin3 (Ultimate Biodegradation Timeframe): 2.5825: Weeks-Months
Biowin4 (Primary Biodegradation Timeframe): 3.5381: Days-Weeks
Biowin5 (MITI Linear Model Prediction) : -0.1821: Does Not Biodegrade Fast
Biowin6 (MITI Non-Linear Model Prediction): 0.0028: Does Not Biodegrade Fast
Biowin7 (Anaerobic Model Prediction): -1.9080: Does Not Biodegrade Fast
Ready Biodegradability Prediction: NO

3.
Biowin1 (Linear Model Prediction) : 0.2277: Does Not Biodegrade Fast
Biowin2 (Non-Linear Model Prediction): 0.0062: Does Not Biodegrade Fast
Biowin3 (Ultimate Biodegradation Timeframe): 2.3727: Weeks-Months
Biowin4 (Primary Biodegradation Timeframe): 3.3268: Days-Weeks
Biowin5 (MITI Linear Model Prediction) : -0.1944: Does Not Biodegrade Fast
Biowin6 (MITI Non-Linear Model Prediction): 0.0013: Does Not Biodegrade Fast
Biowin7 (Anaerobic Model Prediction): -1.5017: Does Not Biodegrade Fast
Ready Biodegradability Prediction: NO

4.
Biowin1 (Linear Model Prediction) : 0.1669: Does Not Biodegrade Fast
Biowin2 (Non-Linear Model Prediction): 0.0041: Does Not Biodegrade Fast
Biowin3 (Ultimate Biodegradation Timeframe): 2.2958: Weeks-Months
Biowin4 (Primary Biodegradation Timeframe): 3.2023: Weeks
Biowin5 (MITI Linear Model Prediction) : -0.1099: Does Not Biodegrade Fast
Biowin6 (MITI Non-Linear Model Prediction): 0.0056: Does Not Biodegrade Fast
Biowin7 (Anaerobic Model Prediction): -1.2351: Does Not Biodegrade Fast
Ready Biodegradability Prediction: NO

5.
Biowin1 (Linear Model Prediction) : 0.3437: Does Not Biodegrade Fast
Biowin2 (Non-Linear Model Prediction): 0.0474: Does Not Biodegrade Fast
Biowin3 (Ultimate Biodegradation Timeframe): 2.7065: Weeks-Months
Biowin4 (Primary Biodegradation Timeframe): 3.6190: Days-Weeks
Biowin5 (MITI Linear Model Prediction) : -0.0638: Does Not Biodegrade Fast
Biowin6 (MITI Non-Linear Model Prediction): 0.0064: Does Not Biodegrade Fast
Biowin7 (Anaerobic Model Prediction): -1.7146: Does Not Biodegrade Fast
Ready Biodegradability Prediction: NO

6.
Biowin1 (Linear Model Prediction) : 0.2553: Does Not Biodegrade Fast
Biowin2 (Non-Linear Model Prediction): 0.0141: Does Not Biodegrade Fast
Biowin3 (Ultimate Biodegradation Timeframe): 2.5012: Weeks-Months
Biowin4 (Primary Biodegradation Timeframe): 3.4107: Days-Weeks
Biowin5 (MITI Linear Model Prediction) : -0.0868: Does Not Biodegrade Fast
Biowin6 (MITI Non-Linear Model Prediction): 0.0060: Does Not Biodegrade Fast
Biowin7 (Anaerobic Model Prediction): -1.4748: Does Not Biodegrade Fast
Ready Biodegradability Prediction: NO

7.
The oxygen consumed by the test systems wascorrected for oxygen consumption occurring in the blank test systems.TheBOD Values (mgO2/mg) and percent biodegradation results for each test system arereported in Tables 2 and 3, respectively. The BOD28 value of test chemical was observed to be 0.188 mgO2/mg. ThoD was determined by calculation as 1.836 mgO2/mg. % Degradation was calculated using the values of BOD and ThOD for test item and was found to be 10.212%. The % degradation of procedure control (Sodium Benzoate) was also calculated using BOD & ThOD and was found to be 74.04%. The mean BOD values(mg O2/l) in control on 28th day was 9 mg O2/l, thereby fulfilling the control validity criteria (i.e., The oxygen consumption of the inoculum blank is normally 20-30 mg O2/l and should not be greater than 60 mg/l in 28 days). Degradation of Sodium Benzoate exceeds 48.04%after 7 days and 66.06% after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid.

8.
Test substance undergoes 1 and 0% degradation by BOD and HPLC parameter in 28 days.
Results with reference substance:
1. to. 6., 8. - No data available

7.
The % degradation of procedure control (Sodium Benzoate) was also calculated using BOD & ThOD and was found to be 74.04%. The mean BOD values(mg O2/l) in control on 28th day was 9 mg O2/l, thereby fulfilling the control validity criteria (i.e., The oxygen consumption of the inoculum blank is normally 20-30 mg O2/l and should not be greater than 60 mg/l in 28 days). Degradation of Sodium Benzoate exceeds 48.04%after 7 days and 66.06% after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid.

1.

BIOWIN (v4.10) Program Results:

==============================

SMILES : O=C(c(c(C(=O)c1c(NCC(CCCC)CC)ccc2NCC(CCCC)CC)ccc3)c3)c12

CHEM : 9,10-Anthracenedione, 1,4-bis[(2-ethylhexyl)amino]-

MOL FOR: C30 H42 N2 O2

MOL WT : 462.68

--------------------------- BIOWIN v4.10 Results ----------------------------

Biowin1 (Linear Model Prediction) : Does Not Biodegrade Fast

Biowin2 (Non-Linear Model Prediction): Does Not Biodegrade Fast

Biowin3 (Ultimate Biodegradation Timeframe): Weeks-Months

Biowin4 (Primary Biodegradation Timeframe): Days-Weeks

Biowin5 (MITI Linear Model Prediction) : Does Not Biodegrade Fast

Biowin6 (MITI Non-Linear Model Prediction): Does Not Biodegrade Fast

Biowin7 (Anaerobic Model Prediction): Does Not Biodegrade Fast

Ready Biodegradability Prediction: NO

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin1 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 0.1084 | 0.2169

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2338 | -0.4675

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.0068 | 0.0137

MolWt| * | Molecular Weight Parameter | | -0.2203

Const| * | Equation Constant | | 0.7475

============+============================================+=========+=========

RESULT | Biowin1 (Linear Biodeg Probability) | | 0.2903

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin2 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 1.8437 | 3.6874

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.9070 | -3.8140

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.4530 | -0.9060

MolWt| * | Molecular Weight Parameter | | -6.5701

============+============================================+=========+=========

RESULT | Biowin2 (Non-Linear Biodeg Probability) | | 0.0100

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin3 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 0.2983 | 0.5967

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1349 | -0.2699

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.0225 | -0.0450

MolWt| * | Molecular Weight Parameter | | -1.0225

Const| * | Equation Constant | | 3.1992

============+============================================+=========+=========

RESULT | Biowin3 (Survey Model - Ultimate Biodeg) | | 2.4585

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin4 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 0.2691 | 0.5381

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1084 | -0.2168

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.0222 | -0.0444

MolWt| * | Molecular Weight Parameter | | -0.6675

Const| * | Equation Constant | | 3.8477

============+============================================+=========+=========

RESULT | Biowin4 (Survey Model - Primary Biodeg) | | 3.4571

============+============================================+=========+=========

Result Classification: 5.00 -> hours 4.00 -> days 3.00 -> weeks

(Primary & Ultimate) 2.00 -> months 1.00 -> longer

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin5 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1577 | -0.3154

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.1177 | 0.2355

Frag | 6 | Aromatic-H | 0.0082 | 0.0493

Frag | 4 | Methyl [-CH3] | 0.0004 | 0.0016

Frag | 10 | -CH2- [linear] | 0.0494 | 0.4942

Frag | 2 | -CH- [linear] | -0.0507 | -0.1013

MolWt| * | Molecular Weight Parameter | | -1.3765

Const| * | Equation Constant | | 0.7121

============+============================================+=========+=========

RESULT | Biowin5 (MITI Linear Biodeg Probability) | | -0.3005

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin6 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.2264 | -2.4527

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.8334 | 1.6669

Frag | 6 | Aromatic-H | 0.1201 | 0.7208

Frag | 4 | Methyl [-CH3] | 0.0194 | 0.0777

Frag | 10 | -CH2- [linear] | 0.4295 | 4.2949

Frag | 2 | -CH- [linear] | -0.0998 | -0.1995

MolWt| * | Molecular Weight Parameter | |-13.3570

============+============================================+=========+=========

RESULT |Biowin6 (MITI Non-Linear Biodeg Probability)| | 0.0012

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Readily Degradable

A Probability Less Than 0.5 indicates --> NOT Readily Degradable

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin7 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | -0.3177 | -0.6355

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2778 | -0.5556

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.3919 | -0.7838

Frag | 6 | Aromatic-H | -0.0954 | -0.5726

Frag | 4 | Methyl [-CH3] | -0.0796 | -0.3183

Frag | 10 | -CH2- [linear] | 0.0260 | 0.2599

Frag | 2 | -CH- [linear] | -0.1659 | -0.3317

Const| * | Equation Constant | | 0.8361

============+============================================+=========+=========

RESULT | Biowin7 (Anaerobic Linear Biodeg Prob) | | -2.1015

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

Ready Biodegradability Prediction: (YES or NO)

----------------------------------------------

Criteria for the YES or NO prediction: If the Biowin3 (ultimate survey

model) result is "weeks" or faster (i.e. "days", "days to weeks", or

"weeks" AND the Biowin5 (MITI linear model) probability is >= 0.5, then

the prediction is YES (readily biodegradable). If this condition is not

satisfied, the prediction is NO (not readily biodegradable). This method

is based on application of Bayesian analysis to ready biodegradation data

(see Help). Biowin5 and 6 also predict ready biodegradability, but for

degradation in the OECD301C test only; using data from the Chemicals

Evaluation and Research Institute Japan (CERIJ) database.

2.

BIOWIN (v4.10) Program Results:

==============================

SMILES : C(CC)(CCCC)CNc1c2c(C(=O)c3c(C2(=O))cccc3)c(cc1)NCCCC

CHEM : 9,10-Anthracenedione, 1-(butylamino)-4-(methylamino)-

MOL FOR: C26 H34 N2 O2

MOL WT : 406.57

--------------------------- BIOWIN v4.10 Results ----------------------------

Biowin1 (Linear Model Prediction) : Does Not Biodegrade Fast

Biowin2 (Non-Linear Model Prediction): Does Not Biodegrade Fast

Biowin3 (Ultimate Biodegradation Timeframe): Weeks-Months

Biowin4 (Primary Biodegradation Timeframe): Days-Weeks

Biowin5 (MITI Linear Model Prediction) : Does Not Biodegrade Fast

Biowin6 (MITI Non-Linear Model Prediction): Does Not Biodegrade Fast

Biowin7 (Anaerobic Model Prediction): Does Not Biodegrade Fast

Ready Biodegradability Prediction: NO

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin1 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 0.1084 | 0.2169

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2338 | -0.4675

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.0068 | 0.0137

MolWt| * | Molecular Weight Parameter | | -0.1936

Const| * | Equation Constant | | 0.7475

============+============================================+=========+=========

RESULT | Biowin1 (Linear Biodeg Probability) | | 0.3170

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin2 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 1.8437 | 3.6874

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.9070 | -3.8140

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.4530 | -0.9060

MolWt| * | Molecular Weight Parameter | | -5.7733

============+============================================+=========+=========

RESULT | Biowin2 (Non-Linear Biodeg Probability) | | 0.0219

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin3 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 0.2983 | 0.5967

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1349 | -0.2699

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.0225 | -0.0450

MolWt| * | Molecular Weight Parameter | | -0.8985

Const| * | Equation Constant | | 3.1992

============+============================================+=========+=========

RESULT | Biowin3 (Survey Model - Ultimate Biodeg) | | 2.5825

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin4 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 0.2691 | 0.5381

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1084 | -0.2168

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.0222 | -0.0444

MolWt| * | Molecular Weight Parameter | | -0.5866

Const| * | Equation Constant | | 3.8477

============+============================================+=========+=========

RESULT | Biowin4 (Survey Model - Primary Biodeg) | | 3.5381

============+============================================+=========+=========

Result Classification: 5.00 -> hours 4.00 -> days 3.00 -> weeks

(Primary & Ultimate) 2.00 -> months 1.00 -> longer

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin5 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1577 | -0.3154

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.1177 | 0.2355

Frag | 6 | Aromatic-H | 0.0082 | 0.0493

Frag | 3 | Methyl [-CH3] | 0.0004 | 0.0012

Frag | 8 | -CH2- [linear] | 0.0494 | 0.3953

Frag | 1 | -CH- [linear] | -0.0507 | -0.0507

MolWt| * | Molecular Weight Parameter | | -1.2096

Const| * | Equation Constant | | 0.7121

============+============================================+=========+=========

RESULT | Biowin5 (MITI Linear Biodeg Probability) | | -0.1821

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin6 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.2264 | -2.4527

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.8334 | 1.6669

Frag | 6 | Aromatic-H | 0.1201 | 0.7208

Frag | 3 | Methyl [-CH3] | 0.0194 | 0.0583

Frag | 8 | -CH2- [linear] | 0.4295 | 3.4360

Frag | 1 | -CH- [linear] | -0.0998 | -0.0998

MolWt| * | Molecular Weight Parameter | |-11.7372

============+============================================+=========+=========

RESULT |Biowin6 (MITI Non-Linear Biodeg Probability)| | 0.0028

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Readily Degradable

A Probability Less Than 0.5 indicates --> NOT Readily Degradable

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin7 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | -0.3177 | -0.6355

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2778 | -0.5556

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.3919 | -0.7838

Frag | 6 | Aromatic-H | -0.0954 | -0.5726

Frag | 3 | Methyl [-CH3] | -0.0796 | -0.2387

Frag | 8 | -CH2- [linear] | 0.0260 | 0.2079

Frag | 1 | -CH- [linear] | -0.1659 | -0.1659

Const| * | Equation Constant | | 0.8361

============+============================================+=========+=========

RESULT | Biowin7 (Anaerobic Linear Biodeg Prob) | | -1.9080

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

Ready Biodegradability Prediction: (YES or NO)

----------------------------------------------

Criteria for the YES or NO prediction: If the Biowin3 (ultimate survey

model) result is "weeks" or faster (i.e. "days", "days to weeks", or

"weeks" AND the Biowin5 (MITI linear model) probability is >= 0.5, then

the prediction is YES (readily biodegradable). If this condition is not

satisfied, the prediction is NO (not readily biodegradable). This method

is based on application of Bayesian analysis to ready biodegradation data

(see Help). Biowin5 and 6 also predict ready biodegradability, but for

degradation in the OECD301C test only; using data from the Chemicals

Evaluation and Research Institute Japan (CERIJ) database.

3.

BIOWIN (v4.10) Program Results:

==============================

SMILES : O=C1c2c(ccc(c2C(=O)C3C1C=CC=C3)NC)NCC(CCCC)CC

CHEM : 9,10-Anthracenedione, 1-[(2-ethylhexyl)amino]-4-(methylamino)-

MOL FOR: C23 H30 N2 O2

MOL WT : 366.51

--------------------------- BIOWIN v4.10 Results ----------------------------

Biowin1 (Linear Model Prediction) : Does Not Biodegrade Fast

Biowin2 (Non-Linear Model Prediction): Does Not Biodegrade Fast

Biowin3 (Ultimate Biodegradation Timeframe): Weeks-Months

Biowin4 (Primary Biodegradation Timeframe): Days-Weeks

Biowin5 (MITI Linear Model Prediction) : Does Not Biodegrade Fast

Biowin6 (MITI Non-Linear Model Prediction): Does Not Biodegrade Fast

Biowin7 (Anaerobic Model Prediction): Does Not Biodegrade Fast

Ready Biodegradability Prediction: NO

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin1 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | 0.1084 | 0.1084

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2338 | -0.4675

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.0068 | 0.0137

MolWt| * | Molecular Weight Parameter | | -0.1745

Const| * | Equation Constant | | 0.7475

============+============================================+=========+=========

RESULT | Biowin1 (Linear Biodeg Probability) | | 0.2277

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin2 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | 1.8437 | 1.8437

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.9070 | -3.8140

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.4530 | -0.9060

MolWt| * | Molecular Weight Parameter | | -5.2044

============+============================================+=========+=========

RESULT | Biowin2 (Non-Linear Biodeg Probability) | | 0.0062

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin3 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | 0.2983 | 0.2983

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1349 | -0.2699

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.0225 | -0.0450

MolWt| * | Molecular Weight Parameter | | -0.8099

Const| * | Equation Constant | | 3.1992

============+============================================+=========+=========

RESULT | Biowin3 (Survey Model - Ultimate Biodeg) | | 2.3727

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin4 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | 0.2691 | 0.2691

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1084 | -0.2168

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.0222 | -0.0444

MolWt| * | Molecular Weight Parameter | | -0.5288

Const| * | Equation Constant | | 3.8477

============+============================================+=========+=========

RESULT | Biowin4 (Survey Model - Primary Biodeg) | | 3.3268

============+============================================+=========+=========

Result Classification: 5.00 -> hours 4.00 -> days 3.00 -> weeks

(Primary & Ultimate) 2.00 -> months 1.00 -> longer

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin5 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1577 | -0.3154

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.1177 | 0.2355

Frag | 2 | Aromatic-H | 0.0082 | 0.0164

Frag | 3 | Methyl [-CH3] | 0.0004 | 0.0012

Frag | 5 | -CH2- [linear] | 0.0494 | 0.2471

Frag | 1 | -CH- [linear] | -0.0507 | -0.0507

Frag | 2 | -CH - [cyclic] | 0.0124 | 0.0249

Frag | 4 | -C=CH [alkenyl hydrogen] | 0.0062 | 0.0248

MolWt| * | Molecular Weight Parameter | | -1.0904

Const| * | Equation Constant | | 0.7121

============+============================================+=========+=========

RESULT | Biowin5 (MITI Linear Biodeg Probability) | | -0.1944

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin6 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.2264 | -2.4527

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.8334 | 1.6669

Frag | 2 | Aromatic-H | 0.1201 | 0.2403

Frag | 3 | Methyl [-CH3] | 0.0194 | 0.0583

Frag | 5 | -CH2- [linear] | 0.4295 | 2.1475

Frag | 1 | -CH- [linear] | -0.0998 | -0.0998

Frag | 2 | -CH - [cyclic] | -0.1295 | -0.2589

Frag | 4 | -C=CH [alkenyl hydrogen] | 0.0285 | 0.1141

MolWt| * | Molecular Weight Parameter | |-10.5806

============+============================================+=========+=========

RESULT |Biowin6 (MITI Non-Linear Biodeg Probability)| | 0.0013

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Readily Degradable

A Probability Less Than 0.5 indicates --> NOT Readily Degradable

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin7 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | -0.3177 | -0.3177

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2778 | -0.5556

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.3919 | -0.7838

Frag | 2 | Aromatic-H | -0.0954 | -0.1909

Frag | 3 | Methyl [-CH3] | -0.0796 | -0.2387

Frag | 5 | -CH2- [linear] | 0.0260 | 0.1299

Frag | 1 | -CH- [linear] | -0.1659 | -0.1659

Frag | 2 | -CH - [cyclic] | 0.0395 | 0.0789

Frag | 4 | -C=CH [alkenyl hydrogen] | -0.0735 | -0.2941

Const| * | Equation Constant | | 0.8361

============+============================================+=========+=========

RESULT | Biowin7 (Anaerobic Linear Biodeg Prob) | | -1.5017

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

Ready Biodegradability Prediction: (YES or NO)

----------------------------------------------

Criteria for the YES or NO prediction: If the Biowin3 (ultimate survey

model) result is "weeks" or faster (i.e. "days", "days to weeks", or

"weeks" AND the Biowin5 (MITI linear model) probability is >= 0.5, then

the prediction is YES (readily biodegradable). If this condition is not

satisfied, the prediction is NO (not readily biodegradable). This method

is based on application of Bayesian analysis to ready biodegradation data

(see Help). Biowin5 and 6 also predict ready biodegradability, but for

degradation in the OECD301C test only; using data from the Chemicals

Evaluation and Research Institute Japan (CERIJ) database.

4.

BIOWIN (v4.10) Program Results:

==============================

SMILES : O=C(c(c(C(=O)c1c(NC)ccc2NC)ccc3)c3)c12

CHEM : 9,10-Anthracenedione, 1,4-bis(methylamino)-

MOL FOR: C16 H14 N2 O2

MOL WT : 266.30

--------------------------- BIOWIN v4.10 Results ----------------------------

Biowin1 (Linear Model Prediction) : Does Not Biodegrade Fast

Biowin2 (Non-Linear Model Prediction): Does Not Biodegrade Fast

Biowin3 (Ultimate Biodegradation Timeframe): Weeks-Months

Biowin4 (Primary Biodegradation Timeframe): Weeks

Biowin5 (MITI Linear Model Prediction) : Does Not Biodegrade Fast

Biowin6 (MITI Non-Linear Model Prediction): Does Not Biodegrade Fast

Biowin7 (Anaerobic Model Prediction): Does Not Biodegrade Fast

Ready Biodegradability Prediction: NO

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin1 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2338 | -0.4675

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.0068 | 0.0137

MolWt| * | Molecular Weight Parameter | | -0.1268

Const| * | Equation Constant | | 0.7475

============+============================================+=========+=========

RESULT | Biowin1 (Linear Biodeg Probability) | | 0.1669

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin2 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.9070 | -3.8140

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.4530 | -0.9060

MolWt| * | Molecular Weight Parameter | | -3.7815

============+============================================+=========+=========

RESULT | Biowin2 (Non-Linear Biodeg Probability) | | 0.0041

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin3 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1349 | -0.2699

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.0225 | -0.0450

MolWt| * | Molecular Weight Parameter | | -0.5885

Const| * | Equation Constant | | 3.1992

============+============================================+=========+=========

RESULT | Biowin3 (Survey Model - Ultimate Biodeg) | | 2.2958

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin4 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1084 | -0.2168

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.0222 | -0.0444

MolWt| * | Molecular Weight Parameter | | -0.3842

Const| * | Equation Constant | | 3.8477

============+============================================+=========+=========

RESULT | Biowin4 (Survey Model - Primary Biodeg) | | 3.2023

============+============================================+=========+=========

Result Classification: 5.00 -> hours 4.00 -> days 3.00 -> weeks

(Primary & Ultimate) 2.00 -> months 1.00 -> longer

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin5 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1577 | -0.3154

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.1177 | 0.2355

Frag | 6 | Aromatic-H | 0.0082 | 0.0493

Frag | 2 | Methyl [-CH3] | 0.0004 | 0.0008

MolWt| * | Molecular Weight Parameter | | -0.7922

Const| * | Equation Constant | | 0.7121

============+============================================+=========+=========

RESULT | Biowin5 (MITI Linear Biodeg Probability) | | -0.1099

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin6 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.2264 | -2.4527

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.8334 | 1.6669

Frag | 6 | Aromatic-H | 0.1201 | 0.7208

Frag | 2 | Methyl [-CH3] | 0.0194 | 0.0389

MolWt| * | Molecular Weight Parameter | | -7.6878

============+============================================+=========+=========

RESULT |Biowin6 (MITI Non-Linear Biodeg Probability)| | 0.0056

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Readily Degradable

A Probability Less Than 0.5 indicates --> NOT Readily Degradable

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin7 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2778 | -0.5556

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.3919 | -0.7838

Frag | 6 | Aromatic-H | -0.0954 | -0.5726

Frag | 2 | Methyl [-CH3] | -0.0796 | -0.1591

Const| * | Equation Constant | | 0.8361

============+============================================+=========+=========

RESULT | Biowin7 (Anaerobic Linear Biodeg Prob) | | -1.2351

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

Ready Biodegradability Prediction: (YES or NO)

----------------------------------------------

Criteria for the YES or NO prediction: If the Biowin3 (ultimate survey

model) result is "weeks" or faster (i.e. "days", "days to weeks", or

"weeks" AND the Biowin5 (MITI linear model) probability is >= 0.5, then

the prediction is YES (readily biodegradable). If this condition is not

satisfied, the prediction is NO (not readily biodegradable). This method

is based on application of Bayesian analysis to ready biodegradation data

(see Help). Biowin5 and 6 also predict ready biodegradability, but for

degradation in the OECD301C test only; using data from the Chemicals

Evaluation and Research Institute Japan (CERIJ) database.

5.

BIOWIN (v4.10) Program Results:

==============================

SMILES : O=C(c(c(C(=O)c1c(NCCCC)ccc2NCCCC)ccc3)c3)c12

CHEM : 9,10-Anthracenedione, 1,4-bis(butylamino)-

MOL FOR: C22 H26 N2 O2

MOL WT : 350.46

--------------------------- BIOWIN v4.10 Results ----------------------------

Biowin1 (Linear Model Prediction) : Does Not Biodegrade Fast

Biowin2 (Non-Linear Model Prediction): Does Not Biodegrade Fast

Biowin3 (Ultimate Biodegradation Timeframe): Weeks-Months

Biowin4 (Primary Biodegradation Timeframe): Days-Weeks

Biowin5 (MITI Linear Model Prediction) : Does Not Biodegrade Fast

Biowin6 (MITI Non-Linear Model Prediction): Does Not Biodegrade Fast

Biowin7 (Anaerobic Model Prediction): Does Not Biodegrade Fast

Ready Biodegradability Prediction: NO

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin1 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 0.1084 | 0.2169

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2338 | -0.4675

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.0068 | 0.0137

MolWt| * | Molecular Weight Parameter | | -0.1668

Const| * | Equation Constant | | 0.7475

============+============================================+=========+=========

RESULT | Biowin1 (Linear Biodeg Probability) | | 0.3437

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin2 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 1.8437 | 3.6874

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.9070 | -3.8140

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.4530 | -0.9060

MolWt| * | Molecular Weight Parameter | | -4.9766

============+============================================+=========+=========

RESULT | Biowin2 (Non-Linear Biodeg Probability) | | 0.0474

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin3 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 0.2983 | 0.5967

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1349 | -0.2699

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.0225 | -0.0450

MolWt| * | Molecular Weight Parameter | | -0.7745

Const| * | Equation Constant | | 3.1992

============+============================================+=========+=========

RESULT | Biowin3 (Survey Model - Ultimate Biodeg) | | 2.7065

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin4 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 0.2691 | 0.5381

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1084 | -0.2168

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.0222 | -0.0444

MolWt| * | Molecular Weight Parameter | | -0.5056

Const| * | Equation Constant | | 3.8477

============+============================================+=========+=========

RESULT | Biowin4 (Survey Model - Primary Biodeg) | | 3.6190

============+============================================+=========+=========

Result Classification: 5.00 -> hours 4.00 -> days 3.00 -> weeks

(Primary & Ultimate) 2.00 -> months 1.00 -> longer

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin5 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1577 | -0.3154

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.1177 | 0.2355

Frag | 6 | Aromatic-H | 0.0082 | 0.0493

Frag | 2 | Methyl [-CH3] | 0.0004 | 0.0008

Frag | 6 | -CH2- [linear] | 0.0494 | 0.2965

MolWt| * | Molecular Weight Parameter | | -1.0426

Const| * | Equation Constant | | 0.7121

============+============================================+=========+=========

RESULT | Biowin5 (MITI Linear Biodeg Probability) | | -0.0638

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin6 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.2264 | -2.4527

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.8334 | 1.6669

Frag | 6 | Aromatic-H | 0.1201 | 0.7208

Frag | 2 | Methyl [-CH3] | 0.0194 | 0.0389

Frag | 6 | -CH2- [linear] | 0.4295 | 2.5770

MolWt| * | Molecular Weight Parameter | |-10.1175

============+============================================+=========+=========

RESULT |Biowin6 (MITI Non-Linear Biodeg Probability)| | 0.0064

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Readily Degradable

A Probability Less Than 0.5 indicates --> NOT Readily Degradable

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin7 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Linear C4 terminal chain [CCC-CH3] | -0.3177 | -0.6355

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2778 | -0.5556

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.3919 | -0.7838

Frag | 6 | Aromatic-H | -0.0954 | -0.5726

Frag | 2 | Methyl [-CH3] | -0.0796 | -0.1591

Frag | 6 | -CH2- [linear] | 0.0260 | 0.1559

Const| * | Equation Constant | | 0.8361

============+============================================+=========+=========

RESULT | Biowin7 (Anaerobic Linear Biodeg Prob) | | -1.7146

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

Ready Biodegradability Prediction: (YES or NO)

----------------------------------------------

Criteria for the YES or NO prediction: If the Biowin3 (ultimate survey

model) result is "weeks" or faster (i.e. "days", "days to weeks", or

"weeks" AND the Biowin5 (MITI linear model) probability is >= 0.5, then

the prediction is YES (readily biodegradable). If this condition is not

satisfied, the prediction is NO (not readily biodegradable). This method

is based on application of Bayesian analysis to ready biodegradation data

(see Help). Biowin5 and 6 also predict ready biodegradability, but for

degradation in the OECD301C test only; using data from the Chemicals

Evaluation and Research Institute Japan (CERIJ) database.

6.

BIOWIN (v4.10) Program Results:

==============================

SMILES : O=C(c(c(C(=O)c1c(NC)ccc2NCCCC)ccc3)c3)c12

CHEM : 9,10-Anthracenedione, 1-(butylamino)-4-(methylamino)-

MOL FOR: C19 H20 N2 O2

MOL WT : 308.38

--------------------------- BIOWIN v4.10 Results ----------------------------

Biowin1 (Linear Model Prediction) : Does Not Biodegrade Fast

Biowin2 (Non-Linear Model Prediction): Does Not Biodegrade Fast

Biowin3 (Ultimate Biodegradation Timeframe): Weeks-Months

Biowin4 (Primary Biodegradation Timeframe): Days-Weeks

Biowin5 (MITI Linear Model Prediction) : Does Not Biodegrade Fast

Biowin6 (MITI Non-Linear Model Prediction): Does Not Biodegrade Fast

Biowin7 (Anaerobic Model Prediction): Does Not Biodegrade Fast

Ready Biodegradability Prediction: NO

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin1 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | 0.1084 | 0.1084

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2338 | -0.4675

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.0068 | 0.0137

MolWt| * | Molecular Weight Parameter | | -0.1468

Const| * | Equation Constant | | 0.7475

============+============================================+=========+=========

RESULT | Biowin1 (Linear Biodeg Probability) | | 0.2553

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin2 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | 1.8437 | 1.8437

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.9070 | -3.8140

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.4530 | -0.9060

MolWt| * | Molecular Weight Parameter | | -4.3790

============+============================================+=========+=========

RESULT | Biowin2 (Non-Linear Biodeg Probability) | | 0.0141

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin3 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | 0.2983 | 0.2983

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1349 | -0.2699

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.0225 | -0.0450

MolWt| * | Molecular Weight Parameter | | -0.6815

Const| * | Equation Constant | | 3.1992

============+============================================+=========+=========

RESULT | Biowin3 (Survey Model - Ultimate Biodeg) | | 2.5012

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin4 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | 0.2691 | 0.2691

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1084 | -0.2168

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.0222 | -0.0444

MolWt| * | Molecular Weight Parameter | | -0.4449

Const| * | Equation Constant | | 3.8477

============+============================================+=========+=========

RESULT | Biowin4 (Survey Model - Primary Biodeg) | | 3.4107

============+============================================+=========+=========

Result Classification: 5.00 -> hours 4.00 -> days 3.00 -> weeks

(Primary & Ultimate) 2.00 -> months 1.00 -> longer

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin5 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.1577 | -0.3154

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.1177 | 0.2355

Frag | 6 | Aromatic-H | 0.0082 | 0.0493

Frag | 2 | Methyl [-CH3] | 0.0004 | 0.0008

Frag | 3 | -CH2- [linear] | 0.0494 | 0.1482

MolWt| * | Molecular Weight Parameter | | -0.9174

Const| * | Equation Constant | | 0.7121

============+============================================+=========+=========

RESULT | Biowin5 (MITI Linear Biodeg Probability) | | -0.0868

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin6 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -1.2264 | -2.4527

Frag | 2 | Ketone [-C-C(=O)-C-] | 0.8334 | 1.6669

Frag | 6 | Aromatic-H | 0.1201 | 0.7208

Frag | 2 | Methyl [-CH3] | 0.0194 | 0.0389

Frag | 3 | -CH2- [linear] | 0.4295 | 1.2885

MolWt| * | Molecular Weight Parameter | | -8.9026

============+============================================+=========+=========

RESULT |Biowin6 (MITI Non-Linear Biodeg Probability)| | 0.0060

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Readily Degradable

A Probability Less Than 0.5 indicates --> NOT Readily Degradable

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin7 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | -0.3177 | -0.3177

Frag | 2 | Aromatic amine [-NH2 or -NH-] | -0.2778 | -0.5556

Frag | 2 | Ketone [-C-C(=O)-C-] | -0.3919 | -0.7838

Frag | 6 | Aromatic-H | -0.0954 | -0.5726

Frag | 2 | Methyl [-CH3] | -0.0796 | -0.1591

Frag | 3 | -CH2- [linear] | 0.0260 | 0.0780

Const| * | Equation Constant | | 0.8361

============+============================================+=========+=========

RESULT | Biowin7 (Anaerobic Linear Biodeg Prob) | | -1.4748

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

Ready Biodegradability Prediction: (YES or NO)

----------------------------------------------

Criteria for the YES or NO prediction: If the Biowin3 (ultimate survey

model) result is "weeks" or faster (i.e. "days", "days to weeks", or

"weeks" AND the Biowin5 (MITI linear model) probability is >= 0.5, then

the prediction is YES (readily biodegradable). If this condition is not

satisfied, the prediction is NO (not readily biodegradable). This method

is based on application of Bayesian analysis to ready biodegradation data

(see Help). Biowin5 and 6 also predict ready biodegradability, but for

degradation in the OECD301C test only; using data from the Chemicals

Evaluation and Research Institute Japan (CERIJ) database.

7.

Table:BOD28, ThOD and % Biodegradation Values

Method details

BOD28 (mgO2/mg)

ThOD (mgO2/mg)

% Biodegradation

pH

0th

Day

pH

28th

Day

Test Item

0.188

1.836

10.212

7.5

7.85

Reference Item

1.233

1.665

74.04

7.0

8.17

Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
On the basis of the predicted data for the test chemical and supporitng experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the percentage degradation of the test chemical Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4-bis[(2-ethylhexyl)amino]anthraquinone and 1-(butylamino)-4-(methylamino)anthraquinone and 1-(butylamino)-4-[(2-ethylhexyl)amino]anthraquinone and 1-[(2-ethylhexyl)amino]-4-(methylamino)anthraquinone can be expected to be range from 0 to 10.212%, respectively in 28 days and half-life was evluated to be 2.2958 to 2.7065 months, respectively. Thus, based on this, test chemical Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4-bis[(2-ethylhexyl)amino]anthraquinone and 1-(butylamino)-4-(methylamino)anthraquinone and 1-(butylamino)-4-[(2-ethylhexyl)amino]anthraquinone and 1-[(2-ethylhexyl)amino]-4-(methylamino)anthraquinone can be considered to be not readily biodegradable in nature.
Executive summary:

Based on the predicted data for the test chemical Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4-bis[(2-ethylhexyl)amino]anthraquinone and 1-(butylamino)-4-(methylamino)anthraquinone and 1-(butylamino)-4-[(2-ethylhexyl)amino]anthraquinone and 1-[(2-ethylhexyl)amino]-4-(methylamino)anthraquinone and different supporting studies for its structurally and functionally similar read across chemical, the biodegradability of the test chemical Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4-bis[(2-ethylhexyl)amino]anthraquinone and 1-(butylamino)-4-(methylamino)anthraquinone and 1-(butylamino)-4-[(2-ethylhexyl)amino]anthraquinone and 1-[(2-ethylhexyl)amino]-4-(methylamino)anthraquinone has been determined. The studies are as mentioned below:

In a prediction using the Estimation Programs Interface Suite, the biodegradation potential of the test compound Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4 -bis(methylamino)anthraquinone and 1,4 -bis[(2 -ethylhexyl)amino]anthraquinone and 1-(butylamino)-4-(methylamino)anthraquinone and 1 -(butylamino)-4 -[(2 -ethylhexyl) amino]anthraquinone and 1 -[(2 -ethylhexyl)amino]-4 -(methylamino)anthraquinone (CAS no. 911 -358 -0) in the presence of mixed populations of environmental microorganisms was predicted. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical 1,4 -bis[(2 -ethylhexyl)amino]-9,10 -dihydro anthracene-9,10 -dione is expected to be not readily biodegradable.

28-days Manometric respirometry test following the OECD guideline 301F was conducted for determining the ready biodegradability of the test chemical. The study was performed at a temperature of 20± 1°C. Mixture of domestic waste water, surface soil and soil samples was used as a test inoculum for the study. This inoculum was collected and were mixed to get diluted suspension. The inoculum was kept aerobic until being used for experiment by supplying organic and inorganic sources required by micro flora to sustain at controlled laboratory conditions.  This gave the bacterial count as 107to 108 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. OECD mineral medium was used for the study. The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 30 mg/L, while that of inoculum was 10 ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (Sodium Benzoate) was also calculated using BOD & ThOD and was determined to be 74.04%. The mean BOD values(mg O2/l) in control on 28th day was 9 mg O2/l, thereby fulfilling the control validity criteria (i.e., The oxygen consumption of the inoculum blank is normally 20-30 mg O2/l and should not be greater than 60 mg/l in 28 days). Degradation of Sodium Benzoate exceeds 48.04%after 7 days and 66.06% after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid. The BOD28 value of test chemical was observed to be 0.188 mgO2sup>/mg. ThOD was calculated as 1.836 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was determined to be 10.212%.Based on the results, the test item, under the test conditions, was considered to be not readily biodegradable at 20 ± 1°C over a period of 28 days.

 

Additional biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively.The percentage degradation of test substance was determined to be 1 and 0% by BOD and HPLC parameter in 28 days.Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

On the basis of the predicted data for the test chemical and supporitng experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the percentage degradation of the test chemical Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4-bis[(2-ethylhexyl)amino]anthraquinone and 1-(butylamino)-4-(methylamino)anthraquinone and 1-(butylamino)-4-[(2-ethylhexyl)amino]anthraquinone and 1 -[(2 -ethylhexyl) amino]-4 -(methylamino)anthraquinone can be expected to be range from 0 to 10.212%, respectively in 28 days and half-life was evluated to be 2.2958 to 2.7065 months, respectively. Thus, based on this, test chemical Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4 -bis[(2 -ethylhexyl)amino] anthraquinone and 1 -(butylamino)-4 -(methylamino)anthraquinone and 1-(butylamino)-4-[(2-ethylhexyl)amino]anthraquinone and 1-[(2-ethylhexyl)amino]-4-(methylamino) anthraquinone can be considered to be not readily biodegradable in nature.

Description of key information

On the basis of the predicted data for the test chemical and supporitng experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the percentage degradation of the test chemical Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4 -bis[(2 -ethylhexyl)amino]anthraquinone and 1 -(butylamino)-4 -(methylamino)anthraquinone and 1 -(butylamino)-4 -[(2 -ethylhexyl)amino]anthraquinone and 1 -[(2 -ethylhexyl)amino]-4 -(methylamino)anthraquinone can be expected to be range from 0 to 10.212%, respectively in 28 days and half-life was evluated to be 2.2958 to 2.7065 months, respectively. Thus, based on this, test chemical Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4-bis[(2-ethylhexyl)amino]anthraquinone and 1-(butylamino)-4-(methylamino)anthraquinone and 1-(butylamino)-4-[(2-ethylhexyl)amino]anthraquinone and 1-[(2-ethylhexyl)amino]-4-(methylamino)anthraquinone can be considered to be not readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Based on the predicted data for the test chemical Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4-bis[(2-ethylhexyl)amino]anthraquinone and 1-(butylamino)-4-(methylamino)anthraquinone and 1-(butylamino)-4-[(2-ethylhexyl)amino]anthraquinone and 1-[(2-ethylhexyl)amino]-4-(methylamino)anthraquinone and different supporting studies for its structurally and functionally similar read across chemical, the biodegradability of the test chemical Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4-bis[(2-ethylhexyl)amino]anthraquinone and 1-(butylamino)-4-(methylamino)anthraquinone and 1-(butylamino)-4-[(2-ethylhexyl)amino]anthraquinone and 1-[(2-ethylhexyl)amino]-4-(methylamino)anthraquinone has been determined. The studies are as mentioned below:

In a prediction using the Estimation Programs Interface Suite, the biodegradation potential of the test compound Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4 -bis(methylamino)anthraquinone and 1,4 -bis[(2 -ethylhexyl)amino]anthraquinone and 1-(butylamino)-4-(methylamino)anthraquinone and 1 -(butylamino)-4 -[(2 -ethylhexyl) amino]anthraquinone and 1 -[(2 -ethylhexyl)amino]-4 -(methylamino)anthraquinone (CAS no. 911 -358 -0) in the presence of mixed populations of environmental microorganisms was predicted. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical 1,4 -bis[(2 -ethylhexyl)amino]-9,10 -dihydro anthracene-9,10 -dione is expected to be not readily biodegradable.

28-days Manometric respirometry test following the OECD guideline 301F was conducted for determining the ready biodegradability of the test chemical. The study was performed at a temperature of 20± 1°C. Mixture of domestic waste water, surface soil and soil samples was used as a test inoculum for the study. This inoculum was collected and were mixed to get diluted suspension. The inoculum was kept aerobic until being used for experiment by supplying organic and inorganic sources required by micro flora to sustain at controlled laboratory conditions.  This gave the bacterial count as 107to 108 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. OECD mineral medium was used for the study. The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 30 mg/L, while that of inoculum was 10 ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (Sodium Benzoate) was also calculated using BOD & ThOD and was determined to be 74.04%. The mean BOD values(mg O2/l) in control on 28th day was 9 mg O2/l, thereby fulfilling the control validity criteria (i.e., The oxygen consumption of the inoculum blank is normally 20-30 mg O2/l and should not be greater than 60 mg/l in 28 days). Degradation of Sodium Benzoate exceeds 48.04%after 7 days and 66.06% after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid. The BOD28 value of test chemical was observed to be 0.188 mgO2sup>/mg. ThOD was calculated as 1.836 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was determined to be 10.212%.Based on the results, the test item, under the test conditions, was considered to be not readily biodegradable at 20 ± 1°C over a period of 28 days.

 

Additional biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively.The percentage degradation of test substance was determined to be 1 and 0% by BOD and HPLC parameter in 28 days.Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

On the basis of the predicted data for the test chemical and supporitng experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the percentage degradation of the test chemical Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4-bis[(2-ethylhexyl)amino]anthraquinone and 1-(butylamino)-4-(methylamino)anthraquinone and 1-(butylamino)-4-[(2-ethylhexyl)amino]anthraquinone and 1 -[(2 -ethylhexyl) amino]-4 -(methylamino)anthraquinone can be expected to be range from 0 to 10.212%, respectively in 28 days and half-life was evluated to be 2.2958 to 2.7065 months, respectively. Thus, based on this, test chemical Reaction mass of 1,4-bis(butylamino)anthraquinone and 1,4-bis(methylamino)anthraquinone and 1,4 -bis[(2 -ethylhexyl)amino] anthraquinone and 1 -(butylamino)-4 -(methylamino)anthraquinone and 1-(butylamino)-4-[(2-ethylhexyl)amino]anthraquinone and 1-[(2-ethylhexyl)amino]-4-(methylamino) anthraquinone can be considered to be not readily biodegradable in nature.