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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Sunflower oil, ester with sorbitol
EC Number:
306-248-9
EC Name:
Sunflower oil, ester with sorbitol
Cas Number:
96690-53-8
IUPAC Name:
Sunflower oil, ester with sorbitol

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Test item treatment: For weighing out the test item was gently heated up to 40 °C to get it homogeneous liquid.

Stock solution: The stock solution (100 mg/L were weighed out) was freshly prepared with dilution water.

Dispersion treatment: Agitation

Limit concentration: 100 mg/L

Test duration 48 h, static

Test vessels Glass beakers (4 cm ID x 7 cm H), 50 ml capacity

Test volume 20 ml

Dilution water ISO Test water, according to OECD 202, Annex 3

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Test organism: Daphnia magna STRAUS (Clone 5)

Reason for the selection of the test organism: Daphnia magna belongs, in accordance with the study guideline, to the suitable Daphnia types and is bred at the test facility.

Origin: lnstitut für Wasser-, Boden- und Lufthygiene (WaBolu)

Breeder: DR. U. NOACK-LABORATORIEN

Culture: In glass vessels (2- 3 L capacity) with approximately 1.8 L culture medium, at 20 ± 2 °C, in an incubator, 16 h illumination, illumination strength max. 20 µE.m-2.s-1.

Culture medium: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L.

Feeding: At least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata, Desmodesmus subspicatus and Chlorela vulgaris, with an alga cell density of> 10^6 cells/ml. The daphnids were not fed during the study.

Number of study organisms: 20 animals, divided into 4 parallel samples, each with 5 animals per limit concentration and control

Age of test organisms: 2 to 24 h old daphnids were used for the definitive study. They were obtained by removing the mother animals twice within 22 h.

Acclimatization: At least 2 h in dilution water

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
160 to 180 mg CaC03/L
Test temperature:
18 - 22 °C, ± 1 °C constant
pH:
7.34-7.63
Dissolved oxygen:
6.72-8.35 mg/l
Conductivity:
683 µS/cm
Nominal and measured concentrations:
Nominal: 100mg/l
Details on test conditions:
Test temperature: 18 - 22 °C, ± 1 °C constant

Illumination: Diffuse light, illumination strength max. 20 µE.m-2.s-1

Photoperiod: 16/8 h light/dark cycle
Reference substance (positive control):
yes
Remarks:
Potassium dichromate p.a. (SIGMA)

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
There was no effect to Daphnia magna observed in the limit concentration and in the control group.
Results with reference substance (positive control):
Test concentrations: 0.625- 1.00- 1.60- 2.56 mg/L
EC50 : 1.66 mg/L (CI 1.65 - 1.66 mg/L)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
At the limit concentration of nominal 100 mg/L of the test item no effect to Daphnia magna was observed.
Executive summary:

In the acute immobilization test with Daphnia magna (STRAUS) the effects of the limit concentration 100 mg/L of the test item were determined according to OECD 202 (2004) at DR.U. NOACK-LABORATORIEN, Kathe-Paulus-Str. 1, D-31157 Sarstedt. The limit test was conducted under static conditions over a period of 48 h from 2009-04-21 to 2009-04-23. 20 test organisms were exposed to the limit concentration and the control. The limit concentration 100 mg/L and the control were analysed via DOC analysis in the fresh media (0 hours) and in the old media (after 48 hours). All effect values are given based on the nominal concentrations of the test item Hostacerin SFO. Water quality parameters of pH-value and dissolved oxygen concentration, measured at 0 and 48 h, were determined to be within acceptable limits. The validity criteria of the test guideline were fulfilled.

At the limit concentration of nominal 100 mg/L of the test item no effect to Daphnia magna was observed.