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EC number: 236-187-2
CAS number: 13215-88-8
The calculated values of the theoretical oxygen demand were 2.86 mg
O2/mg test substance and 1.67 mg O2/mg reference substance.
Table 1. Ready Biodegradability [%] of the test substance and
reference substance (sodium benzoate).
In the inhibition test, all BOD values for the Itr series (test
substance + reference substance) were higher than the sum of the
respective values for the Ir series (reference substance: fatty alcohol)
and the T series (test substance). In conclusion, the test article
exerted no inhibitory effect on the microbial biomass under these test
Not readily biodegradable (3.1% after 28 d, OECD 301 D)
There is one GLP study available, in which the ready
biodegradability of the test substance was assessed according to the
OECD guideline 301 D.
In a closed bottle test microorganisms (2.9E+06 colony forming
units) from the secondary effluent of a local domestic sewage treatment
plant were inoculated with 1.12 mg/L (measured) of the test substance. A
toxicity control was run in parallel to assess the potential inhibitory
effect of the test substance on the microbial biomass.
Total organic carbon was analyzed to determine the actual
concentration of the substance in the initial stock solution. The
biodegradation of the test substance was determined by measuring the
dissolved O2 concentration in the test bottles over 28 d. The
biodegradability of the test substance was then calculated on the basis
of the theoretical oxygen demand.
The results of the toxicity test indicate no inhibitory effect of
the substance on microorganisms at the tested concentration of 1.12
mg/L. The biodegradability of the reference substance was 71.9%,
confirming the suitability of the test system. The biodegradability of
the substance was 3.1% after 28 d. Since the recommended pass level was
not attained by the end of the 28 d inoculation period, the substance is
regarded as not readily biodegradable according to the OECD 301
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