7-anilino-4-hydroxy-3-[[6-methoxy-4-[(6-sulpho-2,4-xylyl)azo]-m-tolyl]azo]naphthalene-2-sulphonic acid, sodium salt, compound with 2,2',2''-nitrilotriethanol

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From January 29th to February 02nd, 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

adopted July 27, 1995

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Water solubility:

68.82 g/L

Conc. based on:

test mat.

Temp.:

20 °C

pH:

8

Details on results:

The water solubility of test item was determined to be 68.82 ± 4.01 g/l (95 % confidence interval) at 20 °C ± 0.5 °C using the flask method for the performance of the main test.
The repeatability of the determination was < 15 % and thus within the range required by the guidelines.

Sampling time	Measured concentration of test item, g/l		Measured pH
	Test vessels	Mean
after 24 hours	65.97	70.60	8
	72.60		8
	73.23		8
after 48 hours	65.33	67.45	8
	69.70		8
	67.33		8
after 72 hours	66.65	68.41	8
	69.64		8
	68.93		8
Mean with confidence interval (95 %)	-	68.82 ± 4.01	-
RSD %	-	2	-

Conclusions:

68.82 ± 4.01 g/l at 20 °C and pH 8

Executive summary:

The water solubility of the test item was determined by flask mehod, follwing the procedures outlined into the OECD guideline 105. About 6.9 g test item was weighed into stoppered Erlenmeyer flasks (nine parallels) according to the estimated solubility based on the Preliminary Test. After the addition of 25 ml ultrapure water, the flasks were agitated at 30 °C. After one day, three of the vessels were removed and re-equilibrated at 20 °C for 24 hours. The content of the vessel was then centrifuged at 20 °C and the concentration in the clear aqueous phase was determined by HPLC. The other flasks were treated similarly after initial equilibration at 30 °C for two and three days, respectively. The samples were diluted before the analysis (two parallel dilutions). The pH of each sample was recorded.

The water solubility of test item was determined to be 68.82 ± 4.01 g/l (95 % confidence interval) at 20 ± 0.5 °C using the flask method for the performance of the main test. The repeatability of the determination was < 15 % and thus within the range required by the guidelines.

Conclusion

68.82 ± 4.01 g/l at 20 °C and pH 8

Description of key information

68.82 ± 4.01 g/l at 20 °C and pH 8

Key value for chemical safety assessment

Water solubility:

68.82 g/L

at the temperature of:

20 °C

Additional information

OECD 105 - flask method