3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooct-1-ene

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 - 24 Aug 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Qualifier:

according to guideline

Guideline:

other: Daphnia sp., Acute immobilization test stipulated in the "Testing Methods for New Chemical Substances" (November 21, 2003; No. 1121002)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control, 100 mg/L

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was added into the dilution water in an Erlenmeyer flask to produce the 100 mg/L test solution. The flask was immediately sealed with a plug not to produce headspace. The solution was stirred by magnetic stirrer for approx. 48 h. After settling for approx. 1 h at 24 ± 1 °C the middle layer of the settled solution was used for testing. The test vessels were covered immediately with a glass lid not to produce headspace.
- Eluate: no
- Differential loading: yes
- Controls: yes, test medium control
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No, test solutions were clear and colorless at test start and before renewal.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: in-house culture in the laboratory (originally obtained from University of Sheffield, UK)
- Age of parental stock (mean and range, SD): 21 d (young daphnids < 24 h were used for the test)
- Feeding during test: none

ACCLIMATION
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Chlorella vulgaris (0.1 - 0.2 mg C/day)
- Feeding frequency: once a day
- Health during acclimation (any mortality observed): No mortality was observed.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

19.9 - 20.0 °C

pH:

7.8 - 7.9

Dissolved oxygen:

8.2 - 8.3 mg/L

Nominal and measured concentrations:

nominal: control, 100 mg/L
measured: < LOD, 0.0719 mg/L (geometric mean)

Details on test conditions:

TEST SYSTEM
- Test vessel: petri dish (diameter: 8 cm, depth: 5 cm)
- Type (delete if not applicable): closed (covered with a petri dish to prevent volatilization of test substance)
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): after 24 h of exposure
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water according to guideline
- Total organic carbon: < 0.1 mg/L
- Chlorine: 15 mg/L (chloride ion)
- Alkalinity: 29 mg/L
- Conductivity: 15.5 mS/m
- Culture medium different from test medium: same as test
- Intervals of water quality measurement: Dissolved oxygen, pH and temperature were measured at test start, before and after renewal and the end of exposure.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
- Light intensity: artificial light of white fluorescent lamp

EFFECT PARAMETERS MEASURED
- Immobility and other symptoms were recorded after 24 and 48 h.

RANGE-FINDING STUDY
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: No effects were observed.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.072 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: 0%
- Abnormal responses: No abnormal response was observed.

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Limit test: no
- Dose-response test: yes
- ECx: EC50 (48 h): 0.296 mg/L (tested in a separate study on 26 - 28 Jun 2007)

Table 1: Measured concentrations of the test item in test solutions

Nominal [mg/L]	Measured concentration [mg/L]
	At test start	24 h		At test end	Geometric mean
		Before renewal	After renewal
Control	< LOQ	< LOQ	< LOQ	< LOQ	-
100	0.0623	0.0629	0.097	0.0704	0.0719

Validity criteria fulfilled:

yes

Remarks:

0% immobilisation in control; O2 content: 8.2 - 8.3 mg/L

Conclusions:

EC50 (48 h) > 0.0719 mg/L (geometric mean)

Description of key information

EC50 (48 h) > 100 mg/L (nominal); > 0.0719 mg/L (meas. (geom. mean))

Additional information

One experimental study is available investigating the short-term effects of 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooct-1-ene (CAS 25291-17-2) to freshwater invertebrates (Daikin Industries, 2007). The study was performed according to OECD 202 (GLP) using Daphnia magna as test organism. Due to the high vapour pressure of the substance (4640 Pa at 25 °C) it was ensured during preparation of test solutions that the substance does not evaporate from the test medium by avoiding any headspace in the Erlenmeyer flasks and the test vessels. The test solution (limit concentration of 100 mg/L) was prepared by adding an appropriate amount of test substance to dilution water followed by 48 h of stirring and a settling period of 1 h. The middle layer was used for testing. Test solutions were renewed after 24 h. To verify the exposure concentrations analytical measurements using GC/MS were applied. After 48 h no mortality was observed resulting in an EC50 of > 100 mg/L (nominal) and > 0.0719 mg/L (meas. (geom. mean)).