Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 696-231-5 | CAS number: 1361000-03-4
Hydrolysis
Administrative data
Link to relevant study record(s)
Description of key information
Percent change in the concentration of C6-Pyrazole hemisulfate in buffer solutions at pH 4, 7 and 9 was greater than 10% with no chromatographic peaks been observed for the test substance after 5 days at 50 ± 0.5°C. Therefore, it was found to be hydrolytically unstable under the test conditions at pH 4, 7 and 9.
Key value for chemical safety assessment
Additional information
The hydrolysis of C6-Pyrazole hemisulfate was determined following OECD Guideline 111 (Hydrolysis as a function of pH).
The hydrolysis of the test substance was determined in 3 buffer solutions (pH 4, 7 and 9) at 50 ± 0.5°C. The analytical procedure, SMV (PC) 3200150-01V, for determining the content of C6-Pyrazole hemisulfate in aqueous buffer solutions was also developed in this study.
Test solutions were prepared by dissolving approximately 16 mg test substance in the buffer solutions (pH 4, 7 or 9) and diluting to 500 mL with the same buffer. From the prepared samples, three vials were filled for each pH level (two for hydrolysis testing and one for pH measurement), along with a fourth vial which was filled with blank buffer for each pH level which served as control. All the vials were incubated for 5 days at 50 ± 0.5°C. The samples from each test and buffer solutions were analyzed at the beginning (Day 0) and after 5 days incubation period by using High Performance Liquid Chromatography with UV detection (HPLC-UV). All the test solutions were diluted 0.5 mL to 1 mL with diluent prior to analysis.
The analytical procedure was successfully validated for the quantification of C6-Pyrazole hemisulfate.
Based on the results, percent change in the concentration of C6-Pyrazole hemisulfate in buffer solutions at pH 4, 7 and 9 was greater than 10% with no chromatographic peaks been observed for the test substance after 5 days at 50 ± 0.5°C. Therefore, it was found to be hydrolytically unstable under the test conditions at pH 4, 7 and 9.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
