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EC number: 615-063-5 | CAS number: 700863-48-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Bioaccumulation: aquatic / sediment
Administrative data
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from August 16, 2009 to October 8, 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: METI Guideline: Concentration Test on Chemical Substances in Fish
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
- Deviations:
- no
- Principles of method if other than guideline:
- SPAN 20 used as solubilizer
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (2R,5S)-2-{4'-[difluoro(3,4,5-trifluorophenoxy)methyl]-3',5'-difluoro-[1,1'-biphenyl]-4-yl}-5-propyloxane
- EC Number:
- 615-063-5
- Cas Number:
- 700863-48-5
- Molecular formula:
- C27H23F7O2
- IUPAC Name:
- (2R,5S)-2-{4'-[difluoro(3,4,5-trifluorophenoxy)methyl]-3',5'-difluoro-[1,1'-biphenyl]-4-yl}-5-propyloxane
Constituent 1
- Specific details on test material used for the study:
- Batch:Dl9202595
Purity: 99.8% (GC) - Radiolabelling:
- no
Sampling and analysis
- Details on sampling:
- - Sampling intervals/frequency for test organisms: days 0, 7, 14, 21, 26, 28
- Sampling intervals/frequency for test medium samples: days 0, 7, 14, 21, 26, 28
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):
Water: Extraction with CH2Cl2 with further HPLC analysis.
Fish: two fish per sampling homogenised, and extracted with acetonitril/n-hexane. The analysis was performed using HPLC.
Test solutions
- Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)): 0.004 and 0.0004 mg/l
Dose 1: Test item: 0.002 mg/L , Vehicle: 0.004 mg/L
Dose 2: Test item: 0.0002 mg/L , Vehicle: 0.0004 mg/L
Vehicle Control: test item: - , Vehicle: 0.004 mg/L
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Common carp Cyprinus carpio (Lot No. 90325)
Supplier Kitamura Carp Breeding Farm (12-388 Gunchiku, Yashiro-shi,
Kumamoto Prefecture, Japan)
Dates purchased March 25, 2009
Feeding and acclimatization:
After receiving the fish at the Institute of Ecotoxicology Co.,
Ltd., those with a total length of approx. 8.0 ± 4.0 cm were
selected (SOP 2.4). After the fish were treated with Elevage ®
( 4-[2-( 5-nitro-2-furanyl)ethenyl]benzoic acid, sodium salt) and
rock salt, the fish were raised. Fish wete selected again on
August 10, 2009 and the fish were transferred to a water-tank.
After another treatment with Elevage, the process of
acclimatization was started (SOP 2.3, SOP 2.5). Finally, fish
were acclimatized at water temperature 25 ± 2.0°C for 7 days
and it was confirmed that the mortality was 5% or less before
use in the test.
Mean bodyweight 3.19 ± 0.33 g
Mean total length 6.9 ± 0.3 cm
Mortality of fish during the experiment
High exposure level 0%
Low exposure level 0%
Control 0%
Lipid contents (by chloroform/methanol extraction method)
Before experiment 5.2% (Mean value, n=2)
After experiment 6.1 % (Mean value, n=2)
Study design
- Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
Test conditions
- Test temperature:
- 25 ° +/- 0.3 °C
- pH:
- 7.6 +/- 0.1
- Dissolved oxygen:
- DO oftest water: 7.5 ± 0.1 mg/L
- TOC:
- High exposure level: 0.6310 mg/L
Low exposure level: 0.6856 mg/L
Control ( dispersant added): 0.4615 mg/L - Salinity:
- According to guideline
- Details on test conditions:
- After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 0, 7, 14, 21, 26 and 28. The analytical values show that the nominal concentrations of 0.002 and 0.0002 mg/L were maintained at about 100% using solubiliser, respectively. At days 0, 7, 14, 21, 26 and 28 two fish each were removed from each test group. Body weight was determined. The fish were homogenised and extracted using organic solvents. The extract was analysed with HPLC. Due to the low bioaccumulation potential, no depuration phase was required.
- Nominal and measured concentrations:
- nominal concentration 0.002 mg/L:
analytically measured concentrations:
day 0: 0.00174 mg/L
day 7: 0.00202 mg/L
day 14: 0.00196 mg/L
day 21: 0.00202 mg/L
day 26: 0.00198mg/L
day 28: 0.00205 mg/L
nominal concentration 0.0002 mg/L
analytically measured concentrations:
day 0: 0.000160 mg/L
day 7: 0.000206 mg/L
day 14: 0.000161 mg/L
day 21: 0.000183 mg/L
day 26: 0.000166 mg/L
day 28: 0.000169 mg/L - Details on estimation of bioconcentration:
- according to guideline
Results and discussion
Bioaccumulation factoropen allclose all
- Conc. / dose:
- 0.002 mg/L
- Temp.:
- 25 °C
- pH:
- 7.7
- Type:
- BCF
- Value:
- 73 dimensionless
- Basis:
- normalised lipid fraction
- Time of plateau:
- 7 d
- Calculation basis:
- steady state
- Conc. / dose:
- 0 mg/L
- Temp.:
- 25 °C
- pH:
- 7.7
- Type:
- BCF
- Value:
- 93 dimensionless
- Basis:
- not specified
- Time of plateau:
- 7 d
- Calculation basis:
- steady state
- Details on kinetic parameters:
- Steady state was reached within 7 days
Due to the low uptake, no depuration phase was necessary - Details on results:
- - Mortality of test organisms: no effects
- Behavioural abnormalities:no effects
- Observations on body length and weight:no effects
- Other biological observations: no effects
- Bound residues forming a plateau: no
- Mortality and/or behavioural abnormalities of control: no
- Loss of test substance during test period: no
- Results with vehicle control: no effects
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 73 and 93 (mean 82).
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