Tar bases, coal, lutidine fraction

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experimental start: 12 January 2016, Experimental termination: 18 February 2016, Finalisation 11 April 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2008

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: WC 2015.10533
- Expiration date of the lot/batch: 31 August 2017
- Purity test date: 99.3% w/w

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature in the dark
- Stability under test conditions: yes
- Solubility and stability of the test substance in the solvent/vehicle: not given
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: no

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: none

Analytical monitoring:

yes

Details on sampling:

Samples were taken from each test solution before the start of the test and after 48 hours. Duplicate samples were taken for further analysis. All samples were stored frozen immediately after sampling.

Vehicle:

no

Details on test solutions:

A concentrated stock solution of nominal 200 mg/L was freshly prepared by dissolving approximately 200 mg of test material into 1000 mL of test water at the start of the study. The rquired test concentrations were prepared as dilutions from this stock solution. The stock solution and the test concentrations were inverted several times to ensure homogeneity and mixing of the solutions. The test media were prepared just before introduction of the daphnids.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna Straus
- Source: A clone of the species was originally supplied by the University of Sheffield in 2002 and since was bred at the laboratory
- Age of parental stock (mean and range, SD): not given
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

not applicable

Hardness:

250 mg/L as CaCO3

Test temperature:

18 - 22 °C

pH:

8.0 to 8.3

Dissolved oxygen:

7.7 to 8.2

Salinity:

not given

Conductivity:

not given

Nominal and measured concentrations:

Nominal: 0 (control), 6.25, 12.5, 25, 50 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL, conical flask
- Type: sealed with ground glass stoppers
- Material, size, headspace, fill volume: glass vessels completely filled with test medium
- Aeration: test water was aerated prior to the start of the study until oxygen saturation was reached, no aeration during the study
- No. of organisms per vessel: 5 daphnids
- No. of vessels per concentration (replicates): 4 parallel
- No. of vessels per control (replicates): a control with 4 vessels was conducted in parallel to studies with test concentrations
- No. of vessels per vehicle control (replicates): not applicable
- Biomass loading rate: not given

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO reconstituted test water was used in the study. It consisted of analytical grade salts dissolved in purified water at the following nominal concentrations. CaCl2 x 2 H2O at 294 mg/L, MgSO4 x 7 H2O at 123 mg/L, NaHCO3 at 65 mg/L, KCl at 5.8 mg/L
- Total organic carbon: not given
- Particulate matter: not given
- Metals: not given
- Pesticides: not given
- Chlorine: not given
- Alkalinity: not given
- Ca/Mg ratio: 4:1
- Conductivity: not given
- Salinity: not given
- Culture medium different from test medium: no
- Intervals of water quality measurement: not given

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: a 16-hour light to 8-hour dark cycle with a 20 minutes transition period was used
- Light intensity: not given

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mobility

VEHICLE CONTROL PERFORMED: no vehicle was used

RANGE-FINDING STUDY
- Test concentrations: A range finding study was conducted at test concentrations of 0.1, 1, 10 and 100 mg/L.
- Results used to determine the conditions for the definitive study: No immobilisation was observed at 0.1, 1 and 10 mg/L, however 70% immobilisation occurred at 100 mg/L. Sub-lethal effects of exposure were also seen at test concentrations of 1, 10 and 100 mg/L. Based on the results, the range of test concentrations for the definitive test were selected.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

The static test on the acute toxicity of the test substance to Daphnia magna resulted in a 48-hour EC50 value (based on mobility) of >100 mg/L.

Executive summary:

The acute aquatic toxicity of the test substance to the waterflea Daphnia magna was studied in a static test in accordance to OECD TG 202 under GLP over a period of 48 hours. The experiment is considered relevant, adequate and conclusive.

Five daphnids were added per test vessel, and the following nominal test concentrations were studied in four parallel replicates: 0 (controls), 6.25, 12.5, 25, 50 and 100 mg/L. The test concentrations were analysed by HPLC with UV detection in samples taken at the start and after 48 hours, and were found to be in the range from 91% to 98% of the nominal test concentration. Therefore, the effect levels were calculated on the basis of nominal concentrations. The 48-hour EC50 value was >100 mg/L in this study, whereas the NOEC was 50 mg/L.

Description of key information

48-hour EC50 >100 mg/L, Daphnia magna, Sacker 2016 (OECD TG 202, GLP)